The effect of hydrostatic pressure on enrichments of hydrocarbon degrading microbes from the Gulf of Mexico following the deepwater Horizon oil spill
© 2018 Marietou, Chastain, Beulig, Scoma, Hazen and Bartlett. The Deepwater Horizon oil spill was one of the largest and deepest oil spills recorded. The wellhead was located at approximately 1500 m below the sea where low temperature and high pressure are key environmental characteristics. Using ce...
Published in: | Frontiers in Microbiology |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
eScholarship, University of California
2018
|
Subjects: | |
Online Access: | http://www.escholarship.org/uc/item/0b63c7k9 |
id |
ftcdlib:qt0b63c7k9 |
---|---|
record_format |
openpolar |
spelling |
ftcdlib:qt0b63c7k9 2023-05-15T13:43:13+02:00 The effect of hydrostatic pressure on enrichments of hydrocarbon degrading microbes from the Gulf of Mexico following the deepwater Horizon oil spill Marietou, A Chastain, R Beulig, F Scoma, A Hazen, TC Bartlett, DH 2018-04-26 application/pdf http://www.escholarship.org/uc/item/0b63c7k9 english eng eScholarship, University of California qt0b63c7k9 http://www.escholarship.org/uc/item/0b63c7k9 public Marietou, A; Chastain, R; Beulig, F; Scoma, A; Hazen, TC; & Bartlett, DH. (2018). The effect of hydrostatic pressure on enrichments of hydrocarbon degrading microbes from the Gulf of Mexico following the deepwater Horizon oil spill. Frontiers in Microbiology, 9(APR). doi:10.3389/fmicb.2018.00808. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/0b63c7k9 article 2018 ftcdlib https://doi.org/10.3389/fmicb.2018.00808 2018-11-02T23:54:11Z © 2018 Marietou, Chastain, Beulig, Scoma, Hazen and Bartlett. The Deepwater Horizon oil spill was one of the largest and deepest oil spills recorded. The wellhead was located at approximately 1500 m below the sea where low temperature and high pressure are key environmental characteristics. Using cells collected 4 months following the Deepwater Horizon oil spill at the Gulf of Mexico, we set up Macondo crude oil enrichments at wellhead temperature and different pressures to determine the effect of increasing depth/pressure to the in situ microbial community and their ability to degrade oil. We observed oil degradation under all pressure conditions tested [0.1, 15, and 30 megapascals (MPa)], although oil degradation profiles, cell numbers, and hydrocarbon degradation gene abundances indicated greatest activity at atmospheric pressure. Under all incubations the growth of psychrophilic bacteria was promoted. Bacteria closely related to Oleispira antarctica RB-8 dominated the communities at all pressures. At 30 MPa we observed a shift toward Photobacterium, a genus that includes piezophiles. Alphaproteobacterial members of the Sulfitobacter, previously associated with oil-degradation, were also highly abundant at 0.1 MPa. Our results suggest that pressure acts synergistically with low temperature to slow microbial growth and thus oil degradation in deep-sea environments. Article in Journal/Newspaper Antarc* Antarctica University of California: eScholarship Frontiers in Microbiology 9 |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
English |
description |
© 2018 Marietou, Chastain, Beulig, Scoma, Hazen and Bartlett. The Deepwater Horizon oil spill was one of the largest and deepest oil spills recorded. The wellhead was located at approximately 1500 m below the sea where low temperature and high pressure are key environmental characteristics. Using cells collected 4 months following the Deepwater Horizon oil spill at the Gulf of Mexico, we set up Macondo crude oil enrichments at wellhead temperature and different pressures to determine the effect of increasing depth/pressure to the in situ microbial community and their ability to degrade oil. We observed oil degradation under all pressure conditions tested [0.1, 15, and 30 megapascals (MPa)], although oil degradation profiles, cell numbers, and hydrocarbon degradation gene abundances indicated greatest activity at atmospheric pressure. Under all incubations the growth of psychrophilic bacteria was promoted. Bacteria closely related to Oleispira antarctica RB-8 dominated the communities at all pressures. At 30 MPa we observed a shift toward Photobacterium, a genus that includes piezophiles. Alphaproteobacterial members of the Sulfitobacter, previously associated with oil-degradation, were also highly abundant at 0.1 MPa. Our results suggest that pressure acts synergistically with low temperature to slow microbial growth and thus oil degradation in deep-sea environments. |
format |
Article in Journal/Newspaper |
author |
Marietou, A Chastain, R Beulig, F Scoma, A Hazen, TC Bartlett, DH |
spellingShingle |
Marietou, A Chastain, R Beulig, F Scoma, A Hazen, TC Bartlett, DH The effect of hydrostatic pressure on enrichments of hydrocarbon degrading microbes from the Gulf of Mexico following the deepwater Horizon oil spill |
author_facet |
Marietou, A Chastain, R Beulig, F Scoma, A Hazen, TC Bartlett, DH |
author_sort |
Marietou, A |
title |
The effect of hydrostatic pressure on enrichments of hydrocarbon degrading microbes from the Gulf of Mexico following the deepwater Horizon oil spill |
title_short |
The effect of hydrostatic pressure on enrichments of hydrocarbon degrading microbes from the Gulf of Mexico following the deepwater Horizon oil spill |
title_full |
The effect of hydrostatic pressure on enrichments of hydrocarbon degrading microbes from the Gulf of Mexico following the deepwater Horizon oil spill |
title_fullStr |
The effect of hydrostatic pressure on enrichments of hydrocarbon degrading microbes from the Gulf of Mexico following the deepwater Horizon oil spill |
title_full_unstemmed |
The effect of hydrostatic pressure on enrichments of hydrocarbon degrading microbes from the Gulf of Mexico following the deepwater Horizon oil spill |
title_sort |
effect of hydrostatic pressure on enrichments of hydrocarbon degrading microbes from the gulf of mexico following the deepwater horizon oil spill |
publisher |
eScholarship, University of California |
publishDate |
2018 |
url |
http://www.escholarship.org/uc/item/0b63c7k9 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Marietou, A; Chastain, R; Beulig, F; Scoma, A; Hazen, TC; & Bartlett, DH. (2018). The effect of hydrostatic pressure on enrichments of hydrocarbon degrading microbes from the Gulf of Mexico following the deepwater Horizon oil spill. Frontiers in Microbiology, 9(APR). doi:10.3389/fmicb.2018.00808. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/0b63c7k9 |
op_relation |
qt0b63c7k9 http://www.escholarship.org/uc/item/0b63c7k9 |
op_rights |
public |
op_doi |
https://doi.org/10.3389/fmicb.2018.00808 |
container_title |
Frontiers in Microbiology |
container_volume |
9 |
_version_ |
1766186049714782208 |