The effect of temperature adaptation on the ubiquitin–proteasome pathway in notothenioid fishes
There is an accumulating body of evidence suggesting that the sub-zero Antarctic marine environment places physiological constraints on protein homeostasis. Levels of ubiquitin (Ub)-conjugated proteins, 20S proteasome activity and mRNA expression of many proteins involved in both the Ub tagging of d...
Main Authors: | , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
eScholarship, University of California
2017
|
Subjects: | |
Online Access: | https://escholarship.org/uc/item/9sg5x3kr |
id |
ftcdlib:oai:escholarship.org:ark:/13030/qt9sg5x3kr |
---|---|
record_format |
openpolar |
spelling |
ftcdlib:oai:escholarship.org:ark:/13030/qt9sg5x3kr 2023-09-05T13:12:22+02:00 The effect of temperature adaptation on the ubiquitin–proteasome pathway in notothenioid fishes Todgham, Anne E Crombie, Timothy A Hofmann, Gretchen E 369 - 378 2017-02-01 application/pdf https://escholarship.org/uc/item/9sg5x3kr unknown eScholarship, University of California qt9sg5x3kr https://escholarship.org/uc/item/9sg5x3kr public Journal of Experimental Biology, vol 220, iss 3 Aetiology 2.1 Biological and endogenous factors Acclimatization Animals Antarctic Regions Cold Temperature Fish Proteins Gene Expression Regulation Perciformes Proteasome Endopeptidase Complex Signal Transduction Ubiquitin Antarctic fish Protein homeostasis Ub-proteasome pathway Cold adaptation Notothenioid Ub–proteasome pathway Biological Sciences Medical and Health Sciences Physiology article 2017 ftcdlib 2023-08-21T18:03:50Z There is an accumulating body of evidence suggesting that the sub-zero Antarctic marine environment places physiological constraints on protein homeostasis. Levels of ubiquitin (Ub)-conjugated proteins, 20S proteasome activity and mRNA expression of many proteins involved in both the Ub tagging of damaged proteins as well as the different complexes of the 26S proteasome were measured to examine whether there is thermal compensation of the Ub-proteasome pathway in Antarctic fishes to better understand the efficiency of the protein degradation machinery in polar species. Both Antarctic (Trematomus bernacchii, Pagothenia borchgrevinki) and non-Antarctic (Notothenia angustata, Bovichtus variegatus) notothenioids were included in this study to investigate the mechanisms of cold adaptation of this pathway in polar species. Overall, there were significant differences in the levels of Ub-conjugated proteins between the Antarctic notothenioids and B. variegatus, with N. angustata possessing levels very similar to those of the Antarctic fishes. Proteasome activity in the gills of Antarctic fishes demonstrated a high degree of temperature compensation such that activity levels were similar to activities measured in their temperate relatives at ecologically relevant temperatures. A similar level of thermal compensation of proteasome activity was not present in the liver of two Antarctic fishes. Higher gill proteasome activity is likely due in part to higher cellular levels of proteins involved in the Ub-proteasome pathway, as evidenced by high mRNA expression of relevant genes. Reduced activity of the Ub-proteasome pathway does not appear to be the mechanism responsible for elevated levels of denatured proteins in Antarctic fishes, at least in the gills. Article in Journal/Newspaper Antarc* Antarctic University of California: eScholarship Antarctic The Antarctic |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Aetiology 2.1 Biological and endogenous factors Acclimatization Animals Antarctic Regions Cold Temperature Fish Proteins Gene Expression Regulation Perciformes Proteasome Endopeptidase Complex Signal Transduction Ubiquitin Antarctic fish Protein homeostasis Ub-proteasome pathway Cold adaptation Notothenioid Ub–proteasome pathway Biological Sciences Medical and Health Sciences Physiology |
spellingShingle |
Aetiology 2.1 Biological and endogenous factors Acclimatization Animals Antarctic Regions Cold Temperature Fish Proteins Gene Expression Regulation Perciformes Proteasome Endopeptidase Complex Signal Transduction Ubiquitin Antarctic fish Protein homeostasis Ub-proteasome pathway Cold adaptation Notothenioid Ub–proteasome pathway Biological Sciences Medical and Health Sciences Physiology Todgham, Anne E Crombie, Timothy A Hofmann, Gretchen E The effect of temperature adaptation on the ubiquitin–proteasome pathway in notothenioid fishes |
topic_facet |
Aetiology 2.1 Biological and endogenous factors Acclimatization Animals Antarctic Regions Cold Temperature Fish Proteins Gene Expression Regulation Perciformes Proteasome Endopeptidase Complex Signal Transduction Ubiquitin Antarctic fish Protein homeostasis Ub-proteasome pathway Cold adaptation Notothenioid Ub–proteasome pathway Biological Sciences Medical and Health Sciences Physiology |
description |
There is an accumulating body of evidence suggesting that the sub-zero Antarctic marine environment places physiological constraints on protein homeostasis. Levels of ubiquitin (Ub)-conjugated proteins, 20S proteasome activity and mRNA expression of many proteins involved in both the Ub tagging of damaged proteins as well as the different complexes of the 26S proteasome were measured to examine whether there is thermal compensation of the Ub-proteasome pathway in Antarctic fishes to better understand the efficiency of the protein degradation machinery in polar species. Both Antarctic (Trematomus bernacchii, Pagothenia borchgrevinki) and non-Antarctic (Notothenia angustata, Bovichtus variegatus) notothenioids were included in this study to investigate the mechanisms of cold adaptation of this pathway in polar species. Overall, there were significant differences in the levels of Ub-conjugated proteins between the Antarctic notothenioids and B. variegatus, with N. angustata possessing levels very similar to those of the Antarctic fishes. Proteasome activity in the gills of Antarctic fishes demonstrated a high degree of temperature compensation such that activity levels were similar to activities measured in their temperate relatives at ecologically relevant temperatures. A similar level of thermal compensation of proteasome activity was not present in the liver of two Antarctic fishes. Higher gill proteasome activity is likely due in part to higher cellular levels of proteins involved in the Ub-proteasome pathway, as evidenced by high mRNA expression of relevant genes. Reduced activity of the Ub-proteasome pathway does not appear to be the mechanism responsible for elevated levels of denatured proteins in Antarctic fishes, at least in the gills. |
format |
Article in Journal/Newspaper |
author |
Todgham, Anne E Crombie, Timothy A Hofmann, Gretchen E |
author_facet |
Todgham, Anne E Crombie, Timothy A Hofmann, Gretchen E |
author_sort |
Todgham, Anne E |
title |
The effect of temperature adaptation on the ubiquitin–proteasome pathway in notothenioid fishes |
title_short |
The effect of temperature adaptation on the ubiquitin–proteasome pathway in notothenioid fishes |
title_full |
The effect of temperature adaptation on the ubiquitin–proteasome pathway in notothenioid fishes |
title_fullStr |
The effect of temperature adaptation on the ubiquitin–proteasome pathway in notothenioid fishes |
title_full_unstemmed |
The effect of temperature adaptation on the ubiquitin–proteasome pathway in notothenioid fishes |
title_sort |
effect of temperature adaptation on the ubiquitin–proteasome pathway in notothenioid fishes |
publisher |
eScholarship, University of California |
publishDate |
2017 |
url |
https://escholarship.org/uc/item/9sg5x3kr |
op_coverage |
369 - 378 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Journal of Experimental Biology, vol 220, iss 3 |
op_relation |
qt9sg5x3kr https://escholarship.org/uc/item/9sg5x3kr |
op_rights |
public |
_version_ |
1776200031199559680 |