Metabolic Capacity of the Antarctic Cyanobacterium Phormidium pseudopriestleyi That Sustains Oxygenic Photosynthesis in the Presence of Hydrogen Sulfide
Sulfide inhibits oxygenic photosynthesis by blocking electron transfer between H2O and the oxygen-evolving complex in the D1 protein of Photosystem II. The ability of cyanobacteria to counter this effect has implications for understanding the productivity of benthic microbial mats in sulfidic enviro...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
eScholarship, University of California
2021
|
Subjects: | |
Online Access: | https://escholarship.org/uc/item/94h0p69v |
id |
ftcdlib:oai:escholarship.org:ark:/13030/qt94h0p69v |
---|---|
record_format |
openpolar |
spelling |
ftcdlib:oai:escholarship.org:ark:/13030/qt94h0p69v 2023-10-01T03:52:07+02:00 Metabolic Capacity of the Antarctic Cyanobacterium Phormidium pseudopriestleyi That Sustains Oxygenic Photosynthesis in the Presence of Hydrogen Sulfide Lumian, Jessica E Jungblut, Anne D Dillion, Megan L Hawes, Ian Doran, Peter T Mackey, Tyler J Dick, Gregory J Grettenberger, Christen L Sumner, Dawn Y 426 2021-01-01 application/pdf https://escholarship.org/uc/item/94h0p69v unknown eScholarship, University of California qt94h0p69v https://escholarship.org/uc/item/94h0p69v public Genes, vol 12, iss 3 Human Genome Genetics Antarctic Regions Bacterial Proteins High-Throughput Nucleotide Sequencing Hydrogen Sulfide Oxygen Phormidium Photosynthesis Sequence Analysis DNA cyanobacteria cryosphere genomics sulfide lake Antarctica article 2021 ftcdlib 2023-09-04T18:04:26Z Sulfide inhibits oxygenic photosynthesis by blocking electron transfer between H2O and the oxygen-evolving complex in the D1 protein of Photosystem II. The ability of cyanobacteria to counter this effect has implications for understanding the productivity of benthic microbial mats in sulfidic environments throughout Earth history. In Lake Fryxell, Antarctica, the benthic, filamentous cyanobacterium Phormidium pseudopriestleyi creates a 1-2 mm thick layer of 50 µmol L-1 O2 in otherwise sulfidic water, demonstrating that it sustains oxygenic photosynthesis in the presence of sulfide. A metagenome-assembled genome of P. pseudopriestleyi indicates a genetic capacity for oxygenic photosynthesis, including multiple copies of psbA (encoding the D1 protein of Photosystem II), and anoxygenic photosynthesis with a copy of sqr (encoding the sulfide quinone reductase protein that oxidizes sulfide). The genomic content of P. pseudopriestleyi is consistent with sulfide tolerance mechanisms including increasing psbA expression or directly oxidizing sulfide with sulfide quinone reductase. However, the ability of the organism to reduce Photosystem I via sulfide quinone reductase while Photosystem II is sulfide-inhibited, thereby performing anoxygenic photosynthesis in the presence of sulfide, has yet to be demonstrated. Article in Journal/Newspaper Antarc* Antarctic Antarctica University of California: eScholarship Antarctic The Antarctic Fryxell ENVELOPE(163.183,163.183,-77.617,-77.617) Lake Fryxell ENVELOPE(163.183,163.183,-77.617,-77.617) |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Human Genome Genetics Antarctic Regions Bacterial Proteins High-Throughput Nucleotide Sequencing Hydrogen Sulfide Oxygen Phormidium Photosynthesis Sequence Analysis DNA cyanobacteria cryosphere genomics sulfide lake Antarctica |
spellingShingle |
Human Genome Genetics Antarctic Regions Bacterial Proteins High-Throughput Nucleotide Sequencing Hydrogen Sulfide Oxygen Phormidium Photosynthesis Sequence Analysis DNA cyanobacteria cryosphere genomics sulfide lake Antarctica Lumian, Jessica E Jungblut, Anne D Dillion, Megan L Hawes, Ian Doran, Peter T Mackey, Tyler J Dick, Gregory J Grettenberger, Christen L Sumner, Dawn Y Metabolic Capacity of the Antarctic Cyanobacterium Phormidium pseudopriestleyi That Sustains Oxygenic Photosynthesis in the Presence of Hydrogen Sulfide |
topic_facet |
Human Genome Genetics Antarctic Regions Bacterial Proteins High-Throughput Nucleotide Sequencing Hydrogen Sulfide Oxygen Phormidium Photosynthesis Sequence Analysis DNA cyanobacteria cryosphere genomics sulfide lake Antarctica |
description |
Sulfide inhibits oxygenic photosynthesis by blocking electron transfer between H2O and the oxygen-evolving complex in the D1 protein of Photosystem II. The ability of cyanobacteria to counter this effect has implications for understanding the productivity of benthic microbial mats in sulfidic environments throughout Earth history. In Lake Fryxell, Antarctica, the benthic, filamentous cyanobacterium Phormidium pseudopriestleyi creates a 1-2 mm thick layer of 50 µmol L-1 O2 in otherwise sulfidic water, demonstrating that it sustains oxygenic photosynthesis in the presence of sulfide. A metagenome-assembled genome of P. pseudopriestleyi indicates a genetic capacity for oxygenic photosynthesis, including multiple copies of psbA (encoding the D1 protein of Photosystem II), and anoxygenic photosynthesis with a copy of sqr (encoding the sulfide quinone reductase protein that oxidizes sulfide). The genomic content of P. pseudopriestleyi is consistent with sulfide tolerance mechanisms including increasing psbA expression or directly oxidizing sulfide with sulfide quinone reductase. However, the ability of the organism to reduce Photosystem I via sulfide quinone reductase while Photosystem II is sulfide-inhibited, thereby performing anoxygenic photosynthesis in the presence of sulfide, has yet to be demonstrated. |
format |
Article in Journal/Newspaper |
author |
Lumian, Jessica E Jungblut, Anne D Dillion, Megan L Hawes, Ian Doran, Peter T Mackey, Tyler J Dick, Gregory J Grettenberger, Christen L Sumner, Dawn Y |
author_facet |
Lumian, Jessica E Jungblut, Anne D Dillion, Megan L Hawes, Ian Doran, Peter T Mackey, Tyler J Dick, Gregory J Grettenberger, Christen L Sumner, Dawn Y |
author_sort |
Lumian, Jessica E |
title |
Metabolic Capacity of the Antarctic Cyanobacterium Phormidium pseudopriestleyi That Sustains Oxygenic Photosynthesis in the Presence of Hydrogen Sulfide |
title_short |
Metabolic Capacity of the Antarctic Cyanobacterium Phormidium pseudopriestleyi That Sustains Oxygenic Photosynthesis in the Presence of Hydrogen Sulfide |
title_full |
Metabolic Capacity of the Antarctic Cyanobacterium Phormidium pseudopriestleyi That Sustains Oxygenic Photosynthesis in the Presence of Hydrogen Sulfide |
title_fullStr |
Metabolic Capacity of the Antarctic Cyanobacterium Phormidium pseudopriestleyi That Sustains Oxygenic Photosynthesis in the Presence of Hydrogen Sulfide |
title_full_unstemmed |
Metabolic Capacity of the Antarctic Cyanobacterium Phormidium pseudopriestleyi That Sustains Oxygenic Photosynthesis in the Presence of Hydrogen Sulfide |
title_sort |
metabolic capacity of the antarctic cyanobacterium phormidium pseudopriestleyi that sustains oxygenic photosynthesis in the presence of hydrogen sulfide |
publisher |
eScholarship, University of California |
publishDate |
2021 |
url |
https://escholarship.org/uc/item/94h0p69v |
op_coverage |
426 |
long_lat |
ENVELOPE(163.183,163.183,-77.617,-77.617) ENVELOPE(163.183,163.183,-77.617,-77.617) |
geographic |
Antarctic The Antarctic Fryxell Lake Fryxell |
geographic_facet |
Antarctic The Antarctic Fryxell Lake Fryxell |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Genes, vol 12, iss 3 |
op_relation |
qt94h0p69v https://escholarship.org/uc/item/94h0p69v |
op_rights |
public |
_version_ |
1778517725308518400 |