Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes
Arbuscular mycorrhizal fungi (AMF, Glomeromycotina), in addition to forming symbioses with the majority of land plants, harbor vertically transmitted endosymbiotic bacteria 'Candidatus Glomeribacter gigasporarum' (CaGg) and 'Candidatus Moeniiplasma glomeromycotorum' (CaMg). CaGg...
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ftcdlib:oai:escholarship.org:ark:/13030/qt8632c4sx 2023-11-05T03:43:50+01:00 Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes Lastovetsky, Olga A Ahn, Ezekiel Mondo, Stephen J Toomer, Kevin H Zhang, Aolin Johnson, Lynn M Pawlowska, Teresa E 3001 - 3013 2018-12-01 application/pdf https://escholarship.org/uc/item/8632c4sx unknown eScholarship, University of California qt8632c4sx https://escholarship.org/uc/item/8632c4sx public The ISME Journal: Multidisciplinary Journal of Microbial Ecology, vol 12, iss 12 Microbiology Biological Sciences Ecology Burkholderiaceae Glomeromycota Massachusetts Mycorrhizae Phylogeny Plant Roots Plants Soil Microbiology Symbiosis Environmental Sciences Technology article 2018 ftcdlib 2023-10-09T18:07:55Z Arbuscular mycorrhizal fungi (AMF, Glomeromycotina), in addition to forming symbioses with the majority of land plants, harbor vertically transmitted endosymbiotic bacteria 'Candidatus Glomeribacter gigasporarum' (CaGg) and 'Candidatus Moeniiplasma glomeromycotorum' (CaMg). CaGg is a nonessential mutualist of AMF, whereas the lifestyle of CaMg is unknown. To start unraveling the interactions between AMF and their endosymbionts in nature, we examined diversity and distribution of AMF-associated endobacteria in North Atlantic dunes at Cape Cod. Of nearly 500 foredune AMF isolates successfully genotyped during a systematic study, 94% were classified as Gigasporaceae. Two percent of all AMF spores harbored CaGg, and 88% contained CaMg. CaGg was found only in the Gigasporaceae, whereas CaMg was present in Gigasporaceae, Acaulosporaceae, and Diversisporaceae. Incidence of CaGg across AMF was not affected by any of the environmental parameters measured, whereas distribution of CaMg in one of the fungal hosts was impacted by plant density. CaMg populations associated with AMF individuals displayed high levels of genetic diversity but no evidence of gene flow, suggesting that host physical proximity is not sufficient to facilitate horizontal transmission of CaMg. Finally, in addition to a novel lineage of CaGg, we discovered that AMF likely harbor Burkholderia-related bacteria with close phylogenetic affinity to free-living Burkholderia and endobacteria of other Mucoromycota fungi. Article in Journal/Newspaper North Atlantic University of California: eScholarship |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Microbiology Biological Sciences Ecology Burkholderiaceae Glomeromycota Massachusetts Mycorrhizae Phylogeny Plant Roots Plants Soil Microbiology Symbiosis Environmental Sciences Technology |
spellingShingle |
Microbiology Biological Sciences Ecology Burkholderiaceae Glomeromycota Massachusetts Mycorrhizae Phylogeny Plant Roots Plants Soil Microbiology Symbiosis Environmental Sciences Technology Lastovetsky, Olga A Ahn, Ezekiel Mondo, Stephen J Toomer, Kevin H Zhang, Aolin Johnson, Lynn M Pawlowska, Teresa E Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes |
topic_facet |
Microbiology Biological Sciences Ecology Burkholderiaceae Glomeromycota Massachusetts Mycorrhizae Phylogeny Plant Roots Plants Soil Microbiology Symbiosis Environmental Sciences Technology |
description |
Arbuscular mycorrhizal fungi (AMF, Glomeromycotina), in addition to forming symbioses with the majority of land plants, harbor vertically transmitted endosymbiotic bacteria 'Candidatus Glomeribacter gigasporarum' (CaGg) and 'Candidatus Moeniiplasma glomeromycotorum' (CaMg). CaGg is a nonessential mutualist of AMF, whereas the lifestyle of CaMg is unknown. To start unraveling the interactions between AMF and their endosymbionts in nature, we examined diversity and distribution of AMF-associated endobacteria in North Atlantic dunes at Cape Cod. Of nearly 500 foredune AMF isolates successfully genotyped during a systematic study, 94% were classified as Gigasporaceae. Two percent of all AMF spores harbored CaGg, and 88% contained CaMg. CaGg was found only in the Gigasporaceae, whereas CaMg was present in Gigasporaceae, Acaulosporaceae, and Diversisporaceae. Incidence of CaGg across AMF was not affected by any of the environmental parameters measured, whereas distribution of CaMg in one of the fungal hosts was impacted by plant density. CaMg populations associated with AMF individuals displayed high levels of genetic diversity but no evidence of gene flow, suggesting that host physical proximity is not sufficient to facilitate horizontal transmission of CaMg. Finally, in addition to a novel lineage of CaGg, we discovered that AMF likely harbor Burkholderia-related bacteria with close phylogenetic affinity to free-living Burkholderia and endobacteria of other Mucoromycota fungi. |
format |
Article in Journal/Newspaper |
author |
Lastovetsky, Olga A Ahn, Ezekiel Mondo, Stephen J Toomer, Kevin H Zhang, Aolin Johnson, Lynn M Pawlowska, Teresa E |
author_facet |
Lastovetsky, Olga A Ahn, Ezekiel Mondo, Stephen J Toomer, Kevin H Zhang, Aolin Johnson, Lynn M Pawlowska, Teresa E |
author_sort |
Lastovetsky, Olga A |
title |
Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes |
title_short |
Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes |
title_full |
Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes |
title_fullStr |
Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes |
title_full_unstemmed |
Distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at North Atlantic dunes |
title_sort |
distribution and population structure of endobacteria in arbuscular mycorrhizal fungi at north atlantic dunes |
publisher |
eScholarship, University of California |
publishDate |
2018 |
url |
https://escholarship.org/uc/item/8632c4sx |
op_coverage |
3001 - 3013 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
The ISME Journal: Multidisciplinary Journal of Microbial Ecology, vol 12, iss 12 |
op_relation |
qt8632c4sx https://escholarship.org/uc/item/8632c4sx |
op_rights |
public |
_version_ |
1781702645212250112 |