Metagenomic insights into diazotrophic communities across Arctic glacier forefields
Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In to...
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ftcdlib:oai:escholarship.org:ark:/13030/qt7kc4x6f3 2023-09-05T13:16:45+02:00 Metagenomic insights into diazotrophic communities across Arctic glacier forefields Nash, Maisie V Anesio, Alexandre M Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G Sánchez-Baracaldo, Patricia fiy114 2018-09-01 https://escholarship.org/uc/item/7kc4x6f3 unknown eScholarship, University of California qt7kc4x6f3 https://escholarship.org/uc/item/7kc4x6f3 public FEMS Microbiology Ecology, vol 94, iss 9 Alphaproteobacteria Arctic Regions Betaproteobacteria Carbon Cyanobacteria Firmicutes Ice Cover Metagenomics Nitrogen Nitrogen Fixation Nitrogenase Phylogeny Soil Soil Microbiology forefield Arctic diversity diazotrophs Environmental Sciences Biological Sciences Medical and Health Sciences Microbiology article 2018 ftcdlib 2023-08-21T18:05:29Z Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes. Article in Journal/Newspaper Arctic University of California: eScholarship Arctic |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Alphaproteobacteria Arctic Regions Betaproteobacteria Carbon Cyanobacteria Firmicutes Ice Cover Metagenomics Nitrogen Nitrogen Fixation Nitrogenase Phylogeny Soil Soil Microbiology forefield Arctic diversity diazotrophs Environmental Sciences Biological Sciences Medical and Health Sciences Microbiology |
spellingShingle |
Alphaproteobacteria Arctic Regions Betaproteobacteria Carbon Cyanobacteria Firmicutes Ice Cover Metagenomics Nitrogen Nitrogen Fixation Nitrogenase Phylogeny Soil Soil Microbiology forefield Arctic diversity diazotrophs Environmental Sciences Biological Sciences Medical and Health Sciences Microbiology Nash, Maisie V Anesio, Alexandre M Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G Sánchez-Baracaldo, Patricia Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
topic_facet |
Alphaproteobacteria Arctic Regions Betaproteobacteria Carbon Cyanobacteria Firmicutes Ice Cover Metagenomics Nitrogen Nitrogen Fixation Nitrogenase Phylogeny Soil Soil Microbiology forefield Arctic diversity diazotrophs Environmental Sciences Biological Sciences Medical and Health Sciences Microbiology |
description |
Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes. |
format |
Article in Journal/Newspaper |
author |
Nash, Maisie V Anesio, Alexandre M Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G Sánchez-Baracaldo, Patricia |
author_facet |
Nash, Maisie V Anesio, Alexandre M Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G Sánchez-Baracaldo, Patricia |
author_sort |
Nash, Maisie V |
title |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_short |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_full |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_fullStr |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_full_unstemmed |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_sort |
metagenomic insights into diazotrophic communities across arctic glacier forefields |
publisher |
eScholarship, University of California |
publishDate |
2018 |
url |
https://escholarship.org/uc/item/7kc4x6f3 |
op_coverage |
fiy114 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
FEMS Microbiology Ecology, vol 94, iss 9 |
op_relation |
qt7kc4x6f3 https://escholarship.org/uc/item/7kc4x6f3 |
op_rights |
public |
_version_ |
1776198221693976576 |