Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis
Little is known at the transcriptional level about microbial eukaryotic adaptations to short-term salinity change. Arctic microalgae are exposed to low salinity due to sea-ice melt and higher salinity with brine channel formation during freeze-up. Here, we investigate the transcriptional response of...
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2022
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ftcdlib:oai:escholarship.org:ark:/13030/qt67t634wv 2024-01-14T10:03:50+01:00 Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis Freyria, Nastasia J Kuo, Alan Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W Grigoriev, Igor V Lovejoy, Connie 500 2022-01-01 application/pdf https://escholarship.org/uc/item/67t634wv unknown eScholarship, University of California qt67t634wv https://escholarship.org/uc/item/67t634wv public Communications Biology, vol 5, iss 1 Biological Sciences Genetics Climate Action Arctic Regions Ice Cover Microalgae Salinity Salt Tolerance Transcriptome Biomedical and clinical sciences article 2022 ftcdlib 2023-12-18T19:06:32Z Little is known at the transcriptional level about microbial eukaryotic adaptations to short-term salinity change. Arctic microalgae are exposed to low salinity due to sea-ice melt and higher salinity with brine channel formation during freeze-up. Here, we investigate the transcriptional response of an ice-associated microalgae over salinities from 45 to 8. Our results show a bracketed response of differential gene expression when the cultures were exposed to progressively decreasing salinity. Key genes associated with salinity changes were involved in specific metabolic pathways, transcription factors and regulators, protein kinases, carbohydrate active enzymes, and inorganic ion transporters. The pelagophyte seemed to use a strategy involving overexpression of Na+-H+ antiporters and Na+ -Pi symporters as salinity decreases, but the K+ channel complex at higher salinities. Specific adaptation to cold saline arctic conditions was seen with differential expression of several antifreeze proteins, an ice-binding protein and an acyl-esterase involved in cold adaptation. Article in Journal/Newspaper Arctic Sea ice University of California: eScholarship Arctic |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Biological Sciences Genetics Climate Action Arctic Regions Ice Cover Microalgae Salinity Salt Tolerance Transcriptome Biomedical and clinical sciences |
spellingShingle |
Biological Sciences Genetics Climate Action Arctic Regions Ice Cover Microalgae Salinity Salt Tolerance Transcriptome Biomedical and clinical sciences Freyria, Nastasia J Kuo, Alan Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W Grigoriev, Igor V Lovejoy, Connie Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
topic_facet |
Biological Sciences Genetics Climate Action Arctic Regions Ice Cover Microalgae Salinity Salt Tolerance Transcriptome Biomedical and clinical sciences |
description |
Little is known at the transcriptional level about microbial eukaryotic adaptations to short-term salinity change. Arctic microalgae are exposed to low salinity due to sea-ice melt and higher salinity with brine channel formation during freeze-up. Here, we investigate the transcriptional response of an ice-associated microalgae over salinities from 45 to 8. Our results show a bracketed response of differential gene expression when the cultures were exposed to progressively decreasing salinity. Key genes associated with salinity changes were involved in specific metabolic pathways, transcription factors and regulators, protein kinases, carbohydrate active enzymes, and inorganic ion transporters. The pelagophyte seemed to use a strategy involving overexpression of Na+-H+ antiporters and Na+ -Pi symporters as salinity decreases, but the K+ channel complex at higher salinities. Specific adaptation to cold saline arctic conditions was seen with differential expression of several antifreeze proteins, an ice-binding protein and an acyl-esterase involved in cold adaptation. |
format |
Article in Journal/Newspaper |
author |
Freyria, Nastasia J Kuo, Alan Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W Grigoriev, Igor V Lovejoy, Connie |
author_facet |
Freyria, Nastasia J Kuo, Alan Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W Grigoriev, Igor V Lovejoy, Connie |
author_sort |
Freyria, Nastasia J |
title |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_short |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_full |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_fullStr |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_full_unstemmed |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_sort |
salinity tolerance mechanisms of an arctic pelagophyte using comparative transcriptomic and gene expression analysis |
publisher |
eScholarship, University of California |
publishDate |
2022 |
url |
https://escholarship.org/uc/item/67t634wv |
op_coverage |
500 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Sea ice |
genre_facet |
Arctic Sea ice |
op_source |
Communications Biology, vol 5, iss 1 |
op_relation |
qt67t634wv https://escholarship.org/uc/item/67t634wv |
op_rights |
public |
_version_ |
1788058510069596160 |