Oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal
The prolonged, postweaning fast of northern elephant seal (Mirounga angustirostris) pups is characterized by a reliance on lipid metabolism and reversible, fasting-induced insulin resistance, providing a unique model to examine the effects of insulin on lipid metabolism. We have previously shown tha...
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ftcdlib:oai:escholarship.org:ark:/13030/qt5cv9b3vb 2024-04-21T08:01:25+00:00 Oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal Wright, Dana N Katundu, Kondwani GH Viscarra, Jose A Crocker, Daniel E Newman, John W La Frano, Michael R Ortiz, Rudy M r537 - r546 2021-10-01 application/pdf https://escholarship.org/uc/item/5cv9b3vb unknown eScholarship, University of California qt5cv9b3vb https://escholarship.org/uc/item/5cv9b3vb public AJP Regulatory Integrative and Comparative Physiology, vol 321, iss 4 Medical Biochemistry and Metabolomics Biomedical and Clinical Sciences Nutrition Diabetes Metabolic and endocrine Animals Biomarkers Fasting Hypoglycemic Agents Infusions Parenteral Insulin Insulin Resistance Lipid Metabolism Oxylipins Seals Earless lipid mediators lipids Biological Sciences Medical and Health Sciences Physiology Health sciences article 2021 ftcdlib 2024-03-27T15:19:54Z The prolonged, postweaning fast of northern elephant seal (Mirounga angustirostris) pups is characterized by a reliance on lipid metabolism and reversible, fasting-induced insulin resistance, providing a unique model to examine the effects of insulin on lipid metabolism. We have previously shown that acute insulin infusion induced a shift in fatty acid metabolism dependent on fasting duration. This study complements the previous study by examining the effects of fasting duration and insulin infusion on circulating levels of oxylipins, bioactive metabolites derived from the oxygenation of polyunsaturated fatty acids. Northern elephant seal pups were studied at two postweaning periods (n = 5/period): early fasting (1-2 wk postweaning; 127 ± 1 kg) and late fasting (6-7 wk postweaning; 93 ± 4 kg). Different cohorts of pups were weighed, sedated, and infused with 65 mU/kg of insulin. Plasma was collected prior to infusion (T0) and at 10, 30, 60, and 120 min postinfusion. A profile of ∼80 oxylipins was analyzed by UPLC-ESI-MS/MS. Nine oxylipins changed between early and late fasting and eight were altered in response to insulin infusion. Fasting decreased prostaglandin F2α (PGF2α) and increased 14,15-dihydroxyicosatrienoic acid (14,15-DiHETrE), 20-hydroxyeicosatetraenoic acid (20-HETE), and 4-hydroxy-docosahexaenoic acid (4-HDoHE) (P < 0.03) in T0 samples, whereas insulin infusion resulted in an inverse change in area-under-the-curve (AUC) levels in these same metabolites (P < 0.05). In addition, 12-12-hydroperoxyeicosatetraenoic acid (HpETE) and 12-HETE decreased with fasting and insulin infusion, respectively (P < 0.04). The oxylipins altered during fasting and in response to insulin infusion may contribute to the manifestation of insulin resistance and participate in the metabolic regulation of associated cellular processes. Article in Journal/Newspaper Elephant Seal University of California: eScholarship |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Medical Biochemistry and Metabolomics Biomedical and Clinical Sciences Nutrition Diabetes Metabolic and endocrine Animals Biomarkers Fasting Hypoglycemic Agents Infusions Parenteral Insulin Insulin Resistance Lipid Metabolism Oxylipins Seals Earless lipid mediators lipids Biological Sciences Medical and Health Sciences Physiology Health sciences |
spellingShingle |
Medical Biochemistry and Metabolomics Biomedical and Clinical Sciences Nutrition Diabetes Metabolic and endocrine Animals Biomarkers Fasting Hypoglycemic Agents Infusions Parenteral Insulin Insulin Resistance Lipid Metabolism Oxylipins Seals Earless lipid mediators lipids Biological Sciences Medical and Health Sciences Physiology Health sciences Wright, Dana N Katundu, Kondwani GH Viscarra, Jose A Crocker, Daniel E Newman, John W La Frano, Michael R Ortiz, Rudy M Oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal |
topic_facet |
Medical Biochemistry and Metabolomics Biomedical and Clinical Sciences Nutrition Diabetes Metabolic and endocrine Animals Biomarkers Fasting Hypoglycemic Agents Infusions Parenteral Insulin Insulin Resistance Lipid Metabolism Oxylipins Seals Earless lipid mediators lipids Biological Sciences Medical and Health Sciences Physiology Health sciences |
description |
The prolonged, postweaning fast of northern elephant seal (Mirounga angustirostris) pups is characterized by a reliance on lipid metabolism and reversible, fasting-induced insulin resistance, providing a unique model to examine the effects of insulin on lipid metabolism. We have previously shown that acute insulin infusion induced a shift in fatty acid metabolism dependent on fasting duration. This study complements the previous study by examining the effects of fasting duration and insulin infusion on circulating levels of oxylipins, bioactive metabolites derived from the oxygenation of polyunsaturated fatty acids. Northern elephant seal pups were studied at two postweaning periods (n = 5/period): early fasting (1-2 wk postweaning; 127 ± 1 kg) and late fasting (6-7 wk postweaning; 93 ± 4 kg). Different cohorts of pups were weighed, sedated, and infused with 65 mU/kg of insulin. Plasma was collected prior to infusion (T0) and at 10, 30, 60, and 120 min postinfusion. A profile of ∼80 oxylipins was analyzed by UPLC-ESI-MS/MS. Nine oxylipins changed between early and late fasting and eight were altered in response to insulin infusion. Fasting decreased prostaglandin F2α (PGF2α) and increased 14,15-dihydroxyicosatrienoic acid (14,15-DiHETrE), 20-hydroxyeicosatetraenoic acid (20-HETE), and 4-hydroxy-docosahexaenoic acid (4-HDoHE) (P < 0.03) in T0 samples, whereas insulin infusion resulted in an inverse change in area-under-the-curve (AUC) levels in these same metabolites (P < 0.05). In addition, 12-12-hydroperoxyeicosatetraenoic acid (HpETE) and 12-HETE decreased with fasting and insulin infusion, respectively (P < 0.04). The oxylipins altered during fasting and in response to insulin infusion may contribute to the manifestation of insulin resistance and participate in the metabolic regulation of associated cellular processes. |
format |
Article in Journal/Newspaper |
author |
Wright, Dana N Katundu, Kondwani GH Viscarra, Jose A Crocker, Daniel E Newman, John W La Frano, Michael R Ortiz, Rudy M |
author_facet |
Wright, Dana N Katundu, Kondwani GH Viscarra, Jose A Crocker, Daniel E Newman, John W La Frano, Michael R Ortiz, Rudy M |
author_sort |
Wright, Dana N |
title |
Oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal |
title_short |
Oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal |
title_full |
Oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal |
title_fullStr |
Oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal |
title_full_unstemmed |
Oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal |
title_sort |
oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal |
publisher |
eScholarship, University of California |
publishDate |
2021 |
url |
https://escholarship.org/uc/item/5cv9b3vb |
op_coverage |
r537 - r546 |
genre |
Elephant Seal |
genre_facet |
Elephant Seal |
op_source |
AJP Regulatory Integrative and Comparative Physiology, vol 321, iss 4 |
op_relation |
qt5cv9b3vb https://escholarship.org/uc/item/5cv9b3vb |
op_rights |
public |
_version_ |
1796941600237027328 |