Change in Emiliania huxleyi Virus Assemblage Diversity but Not in Host Genetic Composition during an Ocean Acidification Mesocosm Experiment
Effects of elevated pCO₂ on Emiliania huxleyi genetic diversity and the viruses that infect E. huxleyi (EhVs) have been investigated in large volume enclosures in a Norwegian fjord. Triplicate enclosures were bubbled with air enriched with CO₂ to 760 ppmv whilst the other three enclosures were bubbl...
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ftcdlib:oai:escholarship.org:ark:/13030/qt4cn569t9 2023-11-05T03:44:25+01:00 Change in Emiliania huxleyi Virus Assemblage Diversity but Not in Host Genetic Composition during an Ocean Acidification Mesocosm Experiment Highfield, Andrea Joint, Ian Gilbert, Jack A Crawfurd, Katharine J Schroeder, Declan C 41 2017-01-01 application/pdf https://escholarship.org/uc/item/4cn569t9 unknown eScholarship, University of California qt4cn569t9 https://escholarship.org/uc/item/4cn569t9 public Viruses, vol 9, iss 3 Biological Sciences Ecology Genetics Life Below Water Carbon Dioxide Chlorophyll Denaturing Gradient Gel Electrophoresis Genetic Variation Haptophyta Nitrates Norway Phosphates Phycodnaviridae Seawater Emiliania huxleyi CO2 ocean acidification climate change Coccolithovirus EhV Microbiology article 2017 ftcdlib 2023-10-09T18:06:18Z Effects of elevated pCO₂ on Emiliania huxleyi genetic diversity and the viruses that infect E. huxleyi (EhVs) have been investigated in large volume enclosures in a Norwegian fjord. Triplicate enclosures were bubbled with air enriched with CO₂ to 760 ppmv whilst the other three enclosures were bubbled with air at ambient pCO₂; phytoplankton growth was initiated by the addition of nitrate and phosphate. E. huxleyi was the dominant coccolithophore in all enclosures, but no difference in genetic diversity, based on DGGE analysis using primers specific to the calcium binding protein gene (gpa) were detected in any of the treatments. Chlorophyll concentrations and primary production were lower in the three elevated pCO₂ treatments than in the ambient treatments. However, although coccolithophores numbers were reduced in two of the high-pCO₂ treatments; in the third, there was no suppression of coccolithophores numbers, which were very similar to the three ambient treatments. In contrast, there was considerable variation in genetic diversity in the EhVs, as determined by analysis of the major capsid protein (mcp) gene. EhV diversity was much lower in the high-pCO₂ treatment enclosure that did not show inhibition of E. huxleyi growth. Since virus infection is generally implicated as a major factor in terminating phytoplankton blooms, it is suggested that no study of the effect of ocean acidification in phytoplankton can be complete if it does not include an assessment of viruses. Article in Journal/Newspaper Ocean acidification University of California: eScholarship |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Biological Sciences Ecology Genetics Life Below Water Carbon Dioxide Chlorophyll Denaturing Gradient Gel Electrophoresis Genetic Variation Haptophyta Nitrates Norway Phosphates Phycodnaviridae Seawater Emiliania huxleyi CO2 ocean acidification climate change Coccolithovirus EhV Microbiology |
spellingShingle |
Biological Sciences Ecology Genetics Life Below Water Carbon Dioxide Chlorophyll Denaturing Gradient Gel Electrophoresis Genetic Variation Haptophyta Nitrates Norway Phosphates Phycodnaviridae Seawater Emiliania huxleyi CO2 ocean acidification climate change Coccolithovirus EhV Microbiology Highfield, Andrea Joint, Ian Gilbert, Jack A Crawfurd, Katharine J Schroeder, Declan C Change in Emiliania huxleyi Virus Assemblage Diversity but Not in Host Genetic Composition during an Ocean Acidification Mesocosm Experiment |
topic_facet |
Biological Sciences Ecology Genetics Life Below Water Carbon Dioxide Chlorophyll Denaturing Gradient Gel Electrophoresis Genetic Variation Haptophyta Nitrates Norway Phosphates Phycodnaviridae Seawater Emiliania huxleyi CO2 ocean acidification climate change Coccolithovirus EhV Microbiology |
description |
Effects of elevated pCO₂ on Emiliania huxleyi genetic diversity and the viruses that infect E. huxleyi (EhVs) have been investigated in large volume enclosures in a Norwegian fjord. Triplicate enclosures were bubbled with air enriched with CO₂ to 760 ppmv whilst the other three enclosures were bubbled with air at ambient pCO₂; phytoplankton growth was initiated by the addition of nitrate and phosphate. E. huxleyi was the dominant coccolithophore in all enclosures, but no difference in genetic diversity, based on DGGE analysis using primers specific to the calcium binding protein gene (gpa) were detected in any of the treatments. Chlorophyll concentrations and primary production were lower in the three elevated pCO₂ treatments than in the ambient treatments. However, although coccolithophores numbers were reduced in two of the high-pCO₂ treatments; in the third, there was no suppression of coccolithophores numbers, which were very similar to the three ambient treatments. In contrast, there was considerable variation in genetic diversity in the EhVs, as determined by analysis of the major capsid protein (mcp) gene. EhV diversity was much lower in the high-pCO₂ treatment enclosure that did not show inhibition of E. huxleyi growth. Since virus infection is generally implicated as a major factor in terminating phytoplankton blooms, it is suggested that no study of the effect of ocean acidification in phytoplankton can be complete if it does not include an assessment of viruses. |
format |
Article in Journal/Newspaper |
author |
Highfield, Andrea Joint, Ian Gilbert, Jack A Crawfurd, Katharine J Schroeder, Declan C |
author_facet |
Highfield, Andrea Joint, Ian Gilbert, Jack A Crawfurd, Katharine J Schroeder, Declan C |
author_sort |
Highfield, Andrea |
title |
Change in Emiliania huxleyi Virus Assemblage Diversity but Not in Host Genetic Composition during an Ocean Acidification Mesocosm Experiment |
title_short |
Change in Emiliania huxleyi Virus Assemblage Diversity but Not in Host Genetic Composition during an Ocean Acidification Mesocosm Experiment |
title_full |
Change in Emiliania huxleyi Virus Assemblage Diversity but Not in Host Genetic Composition during an Ocean Acidification Mesocosm Experiment |
title_fullStr |
Change in Emiliania huxleyi Virus Assemblage Diversity but Not in Host Genetic Composition during an Ocean Acidification Mesocosm Experiment |
title_full_unstemmed |
Change in Emiliania huxleyi Virus Assemblage Diversity but Not in Host Genetic Composition during an Ocean Acidification Mesocosm Experiment |
title_sort |
change in emiliania huxleyi virus assemblage diversity but not in host genetic composition during an ocean acidification mesocosm experiment |
publisher |
eScholarship, University of California |
publishDate |
2017 |
url |
https://escholarship.org/uc/item/4cn569t9 |
op_coverage |
41 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Viruses, vol 9, iss 3 |
op_relation |
qt4cn569t9 https://escholarship.org/uc/item/4cn569t9 |
op_rights |
public |
_version_ |
1781704249043845120 |