Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions
The transition from winter to spring represents a major shift in the basal energy source for the Antarctic marine ecosystem from lipids and other sources of stored energy to sunlight. Because sea ice imposes a strong control on the transmission of sunlight into the water column during the polar spri...
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ftcdlib:oai:escholarship.org:ark:/13030/qt42b6w2hb 2023-10-01T03:50:50+02:00 Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions Bowman, Jeff S Van Mooy, Benjamin AS Lowenstein, Daniel P Fredricks, Helen F Hansel, Colleen M Gast, Rebecca Collins, James R Couto, Nicole Ducklow, Hugh W 2021-01-01 application/pdf https://escholarship.org/uc/item/42b6w2hb unknown eScholarship, University of California qt42b6w2hb https://escholarship.org/uc/item/42b6w2hb public Affordable and Clean Energy Antarctica phytoplankton lipids metatranscriptomics Palmer LTER project Oceanography Ecology article 2021 ftcdlib 2023-09-04T18:03:29Z The transition from winter to spring represents a major shift in the basal energy source for the Antarctic marine ecosystem from lipids and other sources of stored energy to sunlight. Because sea ice imposes a strong control on the transmission of sunlight into the water column during the polar spring, we hypothesized that the timing of the sea ice retreat influences the timing of the transition from stored energy to photosynthesis. To test the influence of sea ice on water column microbial energy utilization we took advantage of unique sea ice conditions in Arthur Harbor, an embayment near Palmer Station on the western Antarctic Peninsula, during the 2015 spring–summer seasonal transition. Over a 5-week period we sampled water from below land-fast sea ice, in the marginal ice zone at nearby Palmer Station B, and conducted an ice removal experiment with incubations of water collected below the land-fast ice. Whole-community metatranscriptomes were paired with lipidomics to better understand how lipid production and utilization was influenced by light conditions. We identified several different phytoplankton taxa that responded similarly to light by the number of genes up-regulated, and in the transcriptional complexity of this response. We applied a principal components analysis to these data to reduce their dimensionality, revealing that each of these taxa exhibited a strikingly different pattern of gene up-regulation. By correlating the changes in lipid concentration to the first principal component of log fold-change for each taxa we could make predictions about which taxa were associated with different changes in the community lipidome. We found that genes coding for the catabolism of triacylglycerol storage lipids were expressed early on in phytoplankton associated with a Fragilariopsis kerguelensis reference transcriptome. Phytoplankton associated with a Corethron pennatum reference transcriptome occupied an adjacent niche, responding favorably to higher light conditions than F. kerguelensis. Other diatom ... Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Sea ice University of California: eScholarship Antarctic Antarctic Peninsula Arthur Harbor ENVELOPE(-64.067,-64.067,-64.767,-64.767) Palmer Station ENVELOPE(-64.050,-64.050,-64.770,-64.770) Palmer-Station ENVELOPE(-64.050,-64.050,-64.770,-64.770) The Antarctic |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Affordable and Clean Energy Antarctica phytoplankton lipids metatranscriptomics Palmer LTER project Oceanography Ecology |
spellingShingle |
Affordable and Clean Energy Antarctica phytoplankton lipids metatranscriptomics Palmer LTER project Oceanography Ecology Bowman, Jeff S Van Mooy, Benjamin AS Lowenstein, Daniel P Fredricks, Helen F Hansel, Colleen M Gast, Rebecca Collins, James R Couto, Nicole Ducklow, Hugh W Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions |
topic_facet |
Affordable and Clean Energy Antarctica phytoplankton lipids metatranscriptomics Palmer LTER project Oceanography Ecology |
description |
The transition from winter to spring represents a major shift in the basal energy source for the Antarctic marine ecosystem from lipids and other sources of stored energy to sunlight. Because sea ice imposes a strong control on the transmission of sunlight into the water column during the polar spring, we hypothesized that the timing of the sea ice retreat influences the timing of the transition from stored energy to photosynthesis. To test the influence of sea ice on water column microbial energy utilization we took advantage of unique sea ice conditions in Arthur Harbor, an embayment near Palmer Station on the western Antarctic Peninsula, during the 2015 spring–summer seasonal transition. Over a 5-week period we sampled water from below land-fast sea ice, in the marginal ice zone at nearby Palmer Station B, and conducted an ice removal experiment with incubations of water collected below the land-fast ice. Whole-community metatranscriptomes were paired with lipidomics to better understand how lipid production and utilization was influenced by light conditions. We identified several different phytoplankton taxa that responded similarly to light by the number of genes up-regulated, and in the transcriptional complexity of this response. We applied a principal components analysis to these data to reduce their dimensionality, revealing that each of these taxa exhibited a strikingly different pattern of gene up-regulation. By correlating the changes in lipid concentration to the first principal component of log fold-change for each taxa we could make predictions about which taxa were associated with different changes in the community lipidome. We found that genes coding for the catabolism of triacylglycerol storage lipids were expressed early on in phytoplankton associated with a Fragilariopsis kerguelensis reference transcriptome. Phytoplankton associated with a Corethron pennatum reference transcriptome occupied an adjacent niche, responding favorably to higher light conditions than F. kerguelensis. Other diatom ... |
format |
Article in Journal/Newspaper |
author |
Bowman, Jeff S Van Mooy, Benjamin AS Lowenstein, Daniel P Fredricks, Helen F Hansel, Colleen M Gast, Rebecca Collins, James R Couto, Nicole Ducklow, Hugh W |
author_facet |
Bowman, Jeff S Van Mooy, Benjamin AS Lowenstein, Daniel P Fredricks, Helen F Hansel, Colleen M Gast, Rebecca Collins, James R Couto, Nicole Ducklow, Hugh W |
author_sort |
Bowman, Jeff S |
title |
Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions |
title_short |
Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions |
title_full |
Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions |
title_fullStr |
Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions |
title_full_unstemmed |
Whole Community Metatranscriptomes and Lipidomes Reveal Diverse Responses Among Antarctic Phytoplankton to Changing Ice Conditions |
title_sort |
whole community metatranscriptomes and lipidomes reveal diverse responses among antarctic phytoplankton to changing ice conditions |
publisher |
eScholarship, University of California |
publishDate |
2021 |
url |
https://escholarship.org/uc/item/42b6w2hb |
long_lat |
ENVELOPE(-64.067,-64.067,-64.767,-64.767) ENVELOPE(-64.050,-64.050,-64.770,-64.770) ENVELOPE(-64.050,-64.050,-64.770,-64.770) |
geographic |
Antarctic Antarctic Peninsula Arthur Harbor Palmer Station Palmer-Station The Antarctic |
geographic_facet |
Antarctic Antarctic Peninsula Arthur Harbor Palmer Station Palmer-Station The Antarctic |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Sea ice |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Sea ice |
op_relation |
qt42b6w2hb https://escholarship.org/uc/item/42b6w2hb |
op_rights |
public |
_version_ |
1778528191093145600 |