Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery
Microbial biosynthetic gene clusters are a valuable source of bioactive molecules. However, because they typically represent a small fraction of genomic material in most metagenomic samples, it remains challenging to deeply sequence them. We present an approach to isolate and sequence gene clusters...
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2020
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ftcdlib:oai:escholarship.org:ark:/13030/qt2rc6v620 2023-09-05T13:15:14+02:00 Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery Xu, Peng Modavi, Cyrus Demaree, Benjamin Twigg, Frederick Liang, Benjamin Sun, Chen Zhang, Wenjun Abate, Adam R e48 - e48 2020-05-07 application/pdf https://escholarship.org/uc/item/2rc6v620 unknown eScholarship, University of California qt2rc6v620 https://escholarship.org/uc/item/2rc6v620 public Nucleic Acids Research, vol 48, iss 8 Human Genome Biotechnology Genetics Bioengineering Biosynthetic Pathways Genomic Library Lab-On-A-Chip Devices Metagenomics Microfluidic Analytical Techniques Plasmids Polyketide Synthases Soil Microbiology Workflow Environmental Sciences Biological Sciences Information and Computing Sciences Developmental Biology article 2020 ftcdlib 2023-08-21T18:03:21Z Microbial biosynthetic gene clusters are a valuable source of bioactive molecules. However, because they typically represent a small fraction of genomic material in most metagenomic samples, it remains challenging to deeply sequence them. We present an approach to isolate and sequence gene clusters in metagenomic samples using microfluidic automated plasmid library enrichment. Our approach provides deep coverage of the target gene cluster, facilitating reassembly. We demonstrate the approach by isolating and sequencing type I polyketide synthase gene clusters from an Antarctic soil metagenome. Our method promotes the discovery of functional-related genes and biosynthetic pathways. Article in Journal/Newspaper Antarc* Antarctic University of California: eScholarship Antarctic |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Human Genome Biotechnology Genetics Bioengineering Biosynthetic Pathways Genomic Library Lab-On-A-Chip Devices Metagenomics Microfluidic Analytical Techniques Plasmids Polyketide Synthases Soil Microbiology Workflow Environmental Sciences Biological Sciences Information and Computing Sciences Developmental Biology |
spellingShingle |
Human Genome Biotechnology Genetics Bioengineering Biosynthetic Pathways Genomic Library Lab-On-A-Chip Devices Metagenomics Microfluidic Analytical Techniques Plasmids Polyketide Synthases Soil Microbiology Workflow Environmental Sciences Biological Sciences Information and Computing Sciences Developmental Biology Xu, Peng Modavi, Cyrus Demaree, Benjamin Twigg, Frederick Liang, Benjamin Sun, Chen Zhang, Wenjun Abate, Adam R Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery |
topic_facet |
Human Genome Biotechnology Genetics Bioengineering Biosynthetic Pathways Genomic Library Lab-On-A-Chip Devices Metagenomics Microfluidic Analytical Techniques Plasmids Polyketide Synthases Soil Microbiology Workflow Environmental Sciences Biological Sciences Information and Computing Sciences Developmental Biology |
description |
Microbial biosynthetic gene clusters are a valuable source of bioactive molecules. However, because they typically represent a small fraction of genomic material in most metagenomic samples, it remains challenging to deeply sequence them. We present an approach to isolate and sequence gene clusters in metagenomic samples using microfluidic automated plasmid library enrichment. Our approach provides deep coverage of the target gene cluster, facilitating reassembly. We demonstrate the approach by isolating and sequencing type I polyketide synthase gene clusters from an Antarctic soil metagenome. Our method promotes the discovery of functional-related genes and biosynthetic pathways. |
format |
Article in Journal/Newspaper |
author |
Xu, Peng Modavi, Cyrus Demaree, Benjamin Twigg, Frederick Liang, Benjamin Sun, Chen Zhang, Wenjun Abate, Adam R |
author_facet |
Xu, Peng Modavi, Cyrus Demaree, Benjamin Twigg, Frederick Liang, Benjamin Sun, Chen Zhang, Wenjun Abate, Adam R |
author_sort |
Xu, Peng |
title |
Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery |
title_short |
Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery |
title_full |
Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery |
title_fullStr |
Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery |
title_full_unstemmed |
Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery |
title_sort |
microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery |
publisher |
eScholarship, University of California |
publishDate |
2020 |
url |
https://escholarship.org/uc/item/2rc6v620 |
op_coverage |
e48 - e48 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Nucleic Acids Research, vol 48, iss 8 |
op_relation |
qt2rc6v620 https://escholarship.org/uc/item/2rc6v620 |
op_rights |
public |
_version_ |
1776197048131911680 |