Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray.
Sentinel fish hornyhead turbot (Pleuronichthysverticalis) captured near wastewater outfalls are used for monitoring exposure to industrial and agricultural chemicals of ~ 20 million people living in coastal Southern California. Although analyses of hormones in blood and organ morphology and histolog...
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ftcdlib:oai:escholarship.org:ark:/13030/qt2fx2w7pq 2023-06-11T04:17:26+02:00 Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. Baker, Michael E Vidal-Dorsch, Doris E Ribecco, Cataldo Sprague, L James Angert, Mila Lekmine, Narimene Ludka, Colleen Martella, Andrea Ricciardelli, Eugenia Bay, Steven M Gully, Joseph R Kelley, Kevin M Schlenk, Daniel Carnevali, Oliana Šášik, Roman Hardiman, Gary Craft, John A e75553 2013-01-01 application/pdf https://escholarship.org/uc/item/2fx2w7pq unknown eScholarship, University of California qt2fx2w7pq https://escholarship.org/uc/item/2fx2w7pq public PloS one, vol 8, iss 9 Liver Zona Pellucida Animals Flatfishes Hormones Isoenzymes Glutathione Transferase Vitellogenins Xenobiotics Water Pollutants Chemical Microarray Analysis Environmental Monitoring Waste Disposal Fluid Gene Expression California Male Endocrine Disruptors Wastewater Estrogen General Science & Technology article 2013 ftcdlib 2023-05-01T17:56:49Z Sentinel fish hornyhead turbot (Pleuronichthysverticalis) captured near wastewater outfalls are used for monitoring exposure to industrial and agricultural chemicals of ~ 20 million people living in coastal Southern California. Although analyses of hormones in blood and organ morphology and histology are useful for assessing contaminant exposure, there is a need for quantitative and sensitive molecular measurements, since contaminants of emerging concern are known to produce subtle effects. We developed a second generation multi-species microarray with expanded content and sensitivity to investigate endocrine disruption in turbot captured near wastewater outfalls in San Diego, Orange County and Los Angeles California. Analysis of expression of genes involved in hormone [e.g., estrogen, androgen, thyroid] responses and xenobiotic metabolism in turbot livers was correlated with a series of phenotypic end points. Molecular analyses of turbot livers uncovered altered expression of vitellogenin and zona pellucida protein, indicating exposure to one or more estrogenic chemicals, as well as, alterations in cytochrome P450 (CYP) 1A, CYP3A and glutathione S-transferase-α indicating induction of the detoxification response. Molecular responses indicative of exposure to endocrine disruptors were observed in field-caught hornyhead turbot captured in Southern California demonstrating the utility of molecular methods for monitoring environmental chemicals in wastewater outfalls. Moreover, this approach can be adapted to monitor other sites for contaminants of emerging concern in other fish species for which there are few available gene sequences. Article in Journal/Newspaper Turbot University of California: eScholarship |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Liver Zona Pellucida Animals Flatfishes Hormones Isoenzymes Glutathione Transferase Vitellogenins Xenobiotics Water Pollutants Chemical Microarray Analysis Environmental Monitoring Waste Disposal Fluid Gene Expression California Male Endocrine Disruptors Wastewater Estrogen General Science & Technology |
spellingShingle |
Liver Zona Pellucida Animals Flatfishes Hormones Isoenzymes Glutathione Transferase Vitellogenins Xenobiotics Water Pollutants Chemical Microarray Analysis Environmental Monitoring Waste Disposal Fluid Gene Expression California Male Endocrine Disruptors Wastewater Estrogen General Science & Technology Baker, Michael E Vidal-Dorsch, Doris E Ribecco, Cataldo Sprague, L James Angert, Mila Lekmine, Narimene Ludka, Colleen Martella, Andrea Ricciardelli, Eugenia Bay, Steven M Gully, Joseph R Kelley, Kevin M Schlenk, Daniel Carnevali, Oliana Šášik, Roman Hardiman, Gary Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. |
topic_facet |
Liver Zona Pellucida Animals Flatfishes Hormones Isoenzymes Glutathione Transferase Vitellogenins Xenobiotics Water Pollutants Chemical Microarray Analysis Environmental Monitoring Waste Disposal Fluid Gene Expression California Male Endocrine Disruptors Wastewater Estrogen General Science & Technology |
description |
Sentinel fish hornyhead turbot (Pleuronichthysverticalis) captured near wastewater outfalls are used for monitoring exposure to industrial and agricultural chemicals of ~ 20 million people living in coastal Southern California. Although analyses of hormones in blood and organ morphology and histology are useful for assessing contaminant exposure, there is a need for quantitative and sensitive molecular measurements, since contaminants of emerging concern are known to produce subtle effects. We developed a second generation multi-species microarray with expanded content and sensitivity to investigate endocrine disruption in turbot captured near wastewater outfalls in San Diego, Orange County and Los Angeles California. Analysis of expression of genes involved in hormone [e.g., estrogen, androgen, thyroid] responses and xenobiotic metabolism in turbot livers was correlated with a series of phenotypic end points. Molecular analyses of turbot livers uncovered altered expression of vitellogenin and zona pellucida protein, indicating exposure to one or more estrogenic chemicals, as well as, alterations in cytochrome P450 (CYP) 1A, CYP3A and glutathione S-transferase-α indicating induction of the detoxification response. Molecular responses indicative of exposure to endocrine disruptors were observed in field-caught hornyhead turbot captured in Southern California demonstrating the utility of molecular methods for monitoring environmental chemicals in wastewater outfalls. Moreover, this approach can be adapted to monitor other sites for contaminants of emerging concern in other fish species for which there are few available gene sequences. |
author2 |
Craft, John A |
format |
Article in Journal/Newspaper |
author |
Baker, Michael E Vidal-Dorsch, Doris E Ribecco, Cataldo Sprague, L James Angert, Mila Lekmine, Narimene Ludka, Colleen Martella, Andrea Ricciardelli, Eugenia Bay, Steven M Gully, Joseph R Kelley, Kevin M Schlenk, Daniel Carnevali, Oliana Šášik, Roman Hardiman, Gary |
author_facet |
Baker, Michael E Vidal-Dorsch, Doris E Ribecco, Cataldo Sprague, L James Angert, Mila Lekmine, Narimene Ludka, Colleen Martella, Andrea Ricciardelli, Eugenia Bay, Steven M Gully, Joseph R Kelley, Kevin M Schlenk, Daniel Carnevali, Oliana Šášik, Roman Hardiman, Gary |
author_sort |
Baker, Michael E |
title |
Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. |
title_short |
Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. |
title_full |
Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. |
title_fullStr |
Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. |
title_full_unstemmed |
Molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. |
title_sort |
molecular analysis of endocrine disruption in hornyhead turbot at wastewater outfalls in southern california using a second generation multi-species microarray. |
publisher |
eScholarship, University of California |
publishDate |
2013 |
url |
https://escholarship.org/uc/item/2fx2w7pq |
op_coverage |
e75553 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
PloS one, vol 8, iss 9 |
op_relation |
qt2fx2w7pq https://escholarship.org/uc/item/2fx2w7pq |
op_rights |
public |
_version_ |
1768376602157121536 |