Plasma FGF21 concentrations, adipose fibroblast growth factor receptor-1 and β-klotho expression decrease with fasting in northern elephant seals.
Fibroblast growth factor (FGF)-21 is secreted from the liver, pancreas, and adipose in response to prolonged fasting/starvation to facilitate lipid and glucose metabolism. Northern elephant seals naturally fast for several months, maintaining a relatively elevated metabolic rate to satisfy their ene...
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ftcdlib:oai:escholarship.org:ark:/13030/qt25x8j9nw 2023-08-27T04:09:15+02:00 Plasma FGF21 concentrations, adipose fibroblast growth factor receptor-1 and β-klotho expression decrease with fasting in northern elephant seals. Suzuki, Miwa Lee, Andrew Y Vázquez-Medina, José Pablo Viscarra, Jose A Crocker, Daniel E Ortiz, Rudy M 2015-05-01 application/pdf https://escholarship.org/uc/item/25x8j9nw unknown eScholarship, University of California qt25x8j9nw https://escholarship.org/uc/item/25x8j9nw public Animals Seals Earless Obesity Glucuronidase Blood Glucose Fibroblast Growth Factors RNA Messenger Enzyme-Linked Immunosorbent Assay Fasting Reverse Transcriptase Polymerase Chain Reaction Food Deprivation Adiposity Receptor Fibroblast Growth Factor Type 1 Real-Time Polymerase Chain Reaction Klotho Proteins Elephant seal Fibroblast growth factor 21 Nutrition Diabetes Metabolic and endocrine Physiology Zoology Veterinary Sciences Endocrinology & Metabolism article 2015 ftcdlib 2023-08-07T18:01:50Z Fibroblast growth factor (FGF)-21 is secreted from the liver, pancreas, and adipose in response to prolonged fasting/starvation to facilitate lipid and glucose metabolism. Northern elephant seals naturally fast for several months, maintaining a relatively elevated metabolic rate to satisfy their energetic requirements. Thus, to better understand the impact of prolonged food deprivation on FGF21-associated changes, we analyzed the expression of FGF21, FGF receptor-1 (FGFR1), β-klotho (KLB; a co-activator of FGFR) in adipose, and plasma FGF21, glucose and 3-hydroxybutyrate in fasted elephant seal pups. Expression of FGFR1 and KLB mRNA decreased 98% and 43%, respectively, with fasting duration. While the 80% decrease in mean adipose FGF21 mRNA expression with fasting did not reach statistical significance, it paralleled the 39% decrease in plasma FGF21 concentrations suggesting that FGF21 is suppressed with fasting in elephant seals. Data demonstrate an atypical response of FGF21 to prolonged fasting in a mammal suggesting that FGF21-mediated mechanisms have evolved differentially in elephant seals. Furthermore, the typical fasting-induced, FGF21-mediated actions such as the inhibition of lipolysis in adipose may not be required in elephant seals as part of a naturally adapted mechanism to support their unique metabolic demands during prolonged fasting. Article in Journal/Newspaper Elephant Seal Elephant Seals University of California: eScholarship |
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Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
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language |
unknown |
topic |
Animals Seals Earless Obesity Glucuronidase Blood Glucose Fibroblast Growth Factors RNA Messenger Enzyme-Linked Immunosorbent Assay Fasting Reverse Transcriptase Polymerase Chain Reaction Food Deprivation Adiposity Receptor Fibroblast Growth Factor Type 1 Real-Time Polymerase Chain Reaction Klotho Proteins Elephant seal Fibroblast growth factor 21 Nutrition Diabetes Metabolic and endocrine Physiology Zoology Veterinary Sciences Endocrinology & Metabolism |
spellingShingle |
Animals Seals Earless Obesity Glucuronidase Blood Glucose Fibroblast Growth Factors RNA Messenger Enzyme-Linked Immunosorbent Assay Fasting Reverse Transcriptase Polymerase Chain Reaction Food Deprivation Adiposity Receptor Fibroblast Growth Factor Type 1 Real-Time Polymerase Chain Reaction Klotho Proteins Elephant seal Fibroblast growth factor 21 Nutrition Diabetes Metabolic and endocrine Physiology Zoology Veterinary Sciences Endocrinology & Metabolism Suzuki, Miwa Lee, Andrew Y Vázquez-Medina, José Pablo Viscarra, Jose A Crocker, Daniel E Ortiz, Rudy M Plasma FGF21 concentrations, adipose fibroblast growth factor receptor-1 and β-klotho expression decrease with fasting in northern elephant seals. |
topic_facet |
Animals Seals Earless Obesity Glucuronidase Blood Glucose Fibroblast Growth Factors RNA Messenger Enzyme-Linked Immunosorbent Assay Fasting Reverse Transcriptase Polymerase Chain Reaction Food Deprivation Adiposity Receptor Fibroblast Growth Factor Type 1 Real-Time Polymerase Chain Reaction Klotho Proteins Elephant seal Fibroblast growth factor 21 Nutrition Diabetes Metabolic and endocrine Physiology Zoology Veterinary Sciences Endocrinology & Metabolism |
description |
Fibroblast growth factor (FGF)-21 is secreted from the liver, pancreas, and adipose in response to prolonged fasting/starvation to facilitate lipid and glucose metabolism. Northern elephant seals naturally fast for several months, maintaining a relatively elevated metabolic rate to satisfy their energetic requirements. Thus, to better understand the impact of prolonged food deprivation on FGF21-associated changes, we analyzed the expression of FGF21, FGF receptor-1 (FGFR1), β-klotho (KLB; a co-activator of FGFR) in adipose, and plasma FGF21, glucose and 3-hydroxybutyrate in fasted elephant seal pups. Expression of FGFR1 and KLB mRNA decreased 98% and 43%, respectively, with fasting duration. While the 80% decrease in mean adipose FGF21 mRNA expression with fasting did not reach statistical significance, it paralleled the 39% decrease in plasma FGF21 concentrations suggesting that FGF21 is suppressed with fasting in elephant seals. Data demonstrate an atypical response of FGF21 to prolonged fasting in a mammal suggesting that FGF21-mediated mechanisms have evolved differentially in elephant seals. Furthermore, the typical fasting-induced, FGF21-mediated actions such as the inhibition of lipolysis in adipose may not be required in elephant seals as part of a naturally adapted mechanism to support their unique metabolic demands during prolonged fasting. |
format |
Article in Journal/Newspaper |
author |
Suzuki, Miwa Lee, Andrew Y Vázquez-Medina, José Pablo Viscarra, Jose A Crocker, Daniel E Ortiz, Rudy M |
author_facet |
Suzuki, Miwa Lee, Andrew Y Vázquez-Medina, José Pablo Viscarra, Jose A Crocker, Daniel E Ortiz, Rudy M |
author_sort |
Suzuki, Miwa |
title |
Plasma FGF21 concentrations, adipose fibroblast growth factor receptor-1 and β-klotho expression decrease with fasting in northern elephant seals. |
title_short |
Plasma FGF21 concentrations, adipose fibroblast growth factor receptor-1 and β-klotho expression decrease with fasting in northern elephant seals. |
title_full |
Plasma FGF21 concentrations, adipose fibroblast growth factor receptor-1 and β-klotho expression decrease with fasting in northern elephant seals. |
title_fullStr |
Plasma FGF21 concentrations, adipose fibroblast growth factor receptor-1 and β-klotho expression decrease with fasting in northern elephant seals. |
title_full_unstemmed |
Plasma FGF21 concentrations, adipose fibroblast growth factor receptor-1 and β-klotho expression decrease with fasting in northern elephant seals. |
title_sort |
plasma fgf21 concentrations, adipose fibroblast growth factor receptor-1 and β-klotho expression decrease with fasting in northern elephant seals. |
publisher |
eScholarship, University of California |
publishDate |
2015 |
url |
https://escholarship.org/uc/item/25x8j9nw |
genre |
Elephant Seal Elephant Seals |
genre_facet |
Elephant Seal Elephant Seals |
op_relation |
qt25x8j9nw https://escholarship.org/uc/item/25x8j9nw |
op_rights |
public |
_version_ |
1775350409439739904 |