Arctic Mustard Flower Color Polymorphism Controlled by Petal-Specific Downregulation at the Threshold of the Anthocyanin Biosynthetic Pathway
Intra- and interspecific variation in flower color is a hallmark of angiosperm diversity. The evolutionary forces underlying the variety of flower colors can be nearly as diverse as the colors themselves. In addition to pollinator preferences, non-pollinator agents of selection can have a major infl...
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ftcdlib:oai:escholarship.org:ark:/13030/qt1pk59079 2023-10-25T01:35:32+02:00 Arctic Mustard Flower Color Polymorphism Controlled by Petal-Specific Downregulation at the Threshold of the Anthocyanin Biosynthetic Pathway Dick, Cynthia A Buenrostro, Jason Butler, Timothy Carlson, Matthew L Kliebenstein, Daniel J Whittall, Justen B Baxter, Ivan e18230 2011-01-01 application/pdf https://escholarship.org/uc/item/1pk59079 unknown eScholarship, University of California qt1pk59079 https://escholarship.org/uc/item/1pk59079 public PLOS ONE, vol 6, iss 4 Agricultural Veterinary and Food Sciences Biological Sciences Horticultural Production Genetics Acyltransferases Alaska Anthocyanins Biosynthetic Pathways Color Flowers Gene Expression Regulation Plant Mustard Plant Reverse Transcriptase Polymerase Chain Reaction General Science & Technology article 2011 ftcdlib 2023-09-25T18:04:31Z Intra- and interspecific variation in flower color is a hallmark of angiosperm diversity. The evolutionary forces underlying the variety of flower colors can be nearly as diverse as the colors themselves. In addition to pollinator preferences, non-pollinator agents of selection can have a major influence on the evolution of flower color polymorphisms, especially when the pigments in question are also expressed in vegetative tissues. In such cases, identifying the target(s) of selection starts with determining the biochemical and molecular basis for the flower color variation and examining any pleiotropic effects manifested in vegetative tissues. Herein, we describe a widespread purple-white flower color polymorphism in the mustard Parrya nudicaulis spanning Alaska. The frequency of white-flowered individuals increases with increasing growing-season temperature, consistent with the role of anthocyanin pigments in stress tolerance. White petals fail to produce the stress responsive flavonoid intermediates in the anthocyanin biosynthetic pathway (ABP), suggesting an early pathway blockage. Petal cDNA sequences did not reveal blockages in any of the eight enzyme-coding genes in white-flowered individuals, nor any color differentiating SNPs. A qRT-PCR analysis of white petals identified a 24-fold reduction in chalcone synthase (CHS) at the threshold of the ABP, but no change in CHS expression in leaves and sepals. This arctic species has avoided the deleterious effects associated with the loss of flavonoid intermediates in vegetative tissues by decoupling CHS expression in petals and leaves, yet the correlation of flower color and climate suggests that the loss of flavonoids in the petals alone may affect the tolerance of white-flowered individuals to colder environments. Article in Journal/Newspaper Arctic Alaska University of California: eScholarship Arctic |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Agricultural Veterinary and Food Sciences Biological Sciences Horticultural Production Genetics Acyltransferases Alaska Anthocyanins Biosynthetic Pathways Color Flowers Gene Expression Regulation Plant Mustard Plant Reverse Transcriptase Polymerase Chain Reaction General Science & Technology |
spellingShingle |
Agricultural Veterinary and Food Sciences Biological Sciences Horticultural Production Genetics Acyltransferases Alaska Anthocyanins Biosynthetic Pathways Color Flowers Gene Expression Regulation Plant Mustard Plant Reverse Transcriptase Polymerase Chain Reaction General Science & Technology Dick, Cynthia A Buenrostro, Jason Butler, Timothy Carlson, Matthew L Kliebenstein, Daniel J Whittall, Justen B Arctic Mustard Flower Color Polymorphism Controlled by Petal-Specific Downregulation at the Threshold of the Anthocyanin Biosynthetic Pathway |
topic_facet |
Agricultural Veterinary and Food Sciences Biological Sciences Horticultural Production Genetics Acyltransferases Alaska Anthocyanins Biosynthetic Pathways Color Flowers Gene Expression Regulation Plant Mustard Plant Reverse Transcriptase Polymerase Chain Reaction General Science & Technology |
description |
Intra- and interspecific variation in flower color is a hallmark of angiosperm diversity. The evolutionary forces underlying the variety of flower colors can be nearly as diverse as the colors themselves. In addition to pollinator preferences, non-pollinator agents of selection can have a major influence on the evolution of flower color polymorphisms, especially when the pigments in question are also expressed in vegetative tissues. In such cases, identifying the target(s) of selection starts with determining the biochemical and molecular basis for the flower color variation and examining any pleiotropic effects manifested in vegetative tissues. Herein, we describe a widespread purple-white flower color polymorphism in the mustard Parrya nudicaulis spanning Alaska. The frequency of white-flowered individuals increases with increasing growing-season temperature, consistent with the role of anthocyanin pigments in stress tolerance. White petals fail to produce the stress responsive flavonoid intermediates in the anthocyanin biosynthetic pathway (ABP), suggesting an early pathway blockage. Petal cDNA sequences did not reveal blockages in any of the eight enzyme-coding genes in white-flowered individuals, nor any color differentiating SNPs. A qRT-PCR analysis of white petals identified a 24-fold reduction in chalcone synthase (CHS) at the threshold of the ABP, but no change in CHS expression in leaves and sepals. This arctic species has avoided the deleterious effects associated with the loss of flavonoid intermediates in vegetative tissues by decoupling CHS expression in petals and leaves, yet the correlation of flower color and climate suggests that the loss of flavonoids in the petals alone may affect the tolerance of white-flowered individuals to colder environments. |
author2 |
Baxter, Ivan |
format |
Article in Journal/Newspaper |
author |
Dick, Cynthia A Buenrostro, Jason Butler, Timothy Carlson, Matthew L Kliebenstein, Daniel J Whittall, Justen B |
author_facet |
Dick, Cynthia A Buenrostro, Jason Butler, Timothy Carlson, Matthew L Kliebenstein, Daniel J Whittall, Justen B |
author_sort |
Dick, Cynthia A |
title |
Arctic Mustard Flower Color Polymorphism Controlled by Petal-Specific Downregulation at the Threshold of the Anthocyanin Biosynthetic Pathway |
title_short |
Arctic Mustard Flower Color Polymorphism Controlled by Petal-Specific Downregulation at the Threshold of the Anthocyanin Biosynthetic Pathway |
title_full |
Arctic Mustard Flower Color Polymorphism Controlled by Petal-Specific Downregulation at the Threshold of the Anthocyanin Biosynthetic Pathway |
title_fullStr |
Arctic Mustard Flower Color Polymorphism Controlled by Petal-Specific Downregulation at the Threshold of the Anthocyanin Biosynthetic Pathway |
title_full_unstemmed |
Arctic Mustard Flower Color Polymorphism Controlled by Petal-Specific Downregulation at the Threshold of the Anthocyanin Biosynthetic Pathway |
title_sort |
arctic mustard flower color polymorphism controlled by petal-specific downregulation at the threshold of the anthocyanin biosynthetic pathway |
publisher |
eScholarship, University of California |
publishDate |
2011 |
url |
https://escholarship.org/uc/item/1pk59079 |
op_coverage |
e18230 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Alaska |
genre_facet |
Arctic Alaska |
op_source |
PLOS ONE, vol 6, iss 4 |
op_relation |
qt1pk59079 https://escholarship.org/uc/item/1pk59079 |
op_rights |
public |
_version_ |
1780730617815629824 |