Microbial Ecology of Atlantic Salmon (Salmo salar) Hatcheries: Impacts of the Built Environment on Fish Mucosal Microbiota.
Successful rearing of fish in hatcheries is critical for conservation, recreational fishing, commercial fishing through wild stock enhancements, and aquaculture production. Flowthrough (FT) hatcheries require more water than recirculating aquaculture systems (RAS), which enable up to 99% of their wa...
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ftcdlib:oai:escholarship.org:ark:/13030/qt19x3q61w 2023-09-05T13:18:11+02:00 Microbial Ecology of Atlantic Salmon (Salmo salar) Hatcheries: Impacts of the Built Environment on Fish Mucosal Microbiota. Minich, Jeremiah J Poore, Greg D Jantawongsri, Khattapan Johnston, Colin Bowie, Kate Bowman, John Knight, Rob Nowak, Barbara Allen, Eric E Liu, Shuang-Jiang e00411 - e00420 2020-06-02 application/pdf https://escholarship.org/uc/item/19x3q61w unknown eScholarship, University of California qt19x3q61w https://escholarship.org/uc/item/19x3q61w public Applied and Environmental Microbiology, vol 86, iss 12 Life Below Water Animals Aquaculture Biofilms Built Environment Fisheries Gastrointestinal Contents Gills Microbiota Mucous Membrane Salmo salar Skin Water Microbiology 16S environmental microbiology microbial ecology microbiome Microbiology article 2020 ftcdlib 2023-08-21T18:07:21Z Successful rearing of fish in hatcheries is critical for conservation, recreational fishing, commercial fishing through wild stock enhancements, and aquaculture production. Flowthrough (FT) hatcheries require more water than recirculating aquaculture systems (RAS), which enable up to 99% of their water to be recycled, thus significantly reducing environmental impacts. Here, we evaluated the biological and physical microbiome interactions of three Atlantic salmon hatcheries (RAS n = 2, FT n = 1). Gill, skin, and digesta from six juvenile fish along with tank biofilms and water were sampled from tanks in each of the hatcheries (60 fish across 10 tanks) to assess the built environment and mucosal microbiota using 16S rRNA gene sequencing. The water and tank biofilm had more microbial richness than fish mucus, while skin and digesta from RAS fish had 2 times the richness of FT fish. Body sites each had unique microbiomes (P < 0.001) and were influenced by hatchery system type (P < 0.001), with RAS being more similar. A strong association between the tank and fish microbiome was observed. Water and tank biofilm richness was positively correlated with skin and digesta richness. Strikingly, the gill, skin, and digesta communities were more similar to that in the origin tank biofilm than those in all other experimental tanks, suggesting that the tank biofilm has a direct influence on fish-associated microbial communities. Lastly, microbial diversity and mucous cell density were positively associated with fish growth and length. The results from this study provide evidence for a link between the tank microbiome and the fish microbiome, with the skin microbiome as an important intermediate.IMPORTANCE Atlantic salmon, Salmo salar, is the most farmed marine fish worldwide, with an annual production of 2,248 million metric tons in 2016. Salmon hatcheries are increasingly changing from flowthrough toward recirculating aquaculture system (RAS) design to accommodate more control over production along with improved ... Article in Journal/Newspaper Atlantic salmon Salmo salar University of California: eScholarship |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Life Below Water Animals Aquaculture Biofilms Built Environment Fisheries Gastrointestinal Contents Gills Microbiota Mucous Membrane Salmo salar Skin Water Microbiology 16S environmental microbiology microbial ecology microbiome Microbiology |
spellingShingle |
Life Below Water Animals Aquaculture Biofilms Built Environment Fisheries Gastrointestinal Contents Gills Microbiota Mucous Membrane Salmo salar Skin Water Microbiology 16S environmental microbiology microbial ecology microbiome Microbiology Minich, Jeremiah J Poore, Greg D Jantawongsri, Khattapan Johnston, Colin Bowie, Kate Bowman, John Knight, Rob Nowak, Barbara Allen, Eric E Microbial Ecology of Atlantic Salmon (Salmo salar) Hatcheries: Impacts of the Built Environment on Fish Mucosal Microbiota. |
topic_facet |
Life Below Water Animals Aquaculture Biofilms Built Environment Fisheries Gastrointestinal Contents Gills Microbiota Mucous Membrane Salmo salar Skin Water Microbiology 16S environmental microbiology microbial ecology microbiome Microbiology |
description |
Successful rearing of fish in hatcheries is critical for conservation, recreational fishing, commercial fishing through wild stock enhancements, and aquaculture production. Flowthrough (FT) hatcheries require more water than recirculating aquaculture systems (RAS), which enable up to 99% of their water to be recycled, thus significantly reducing environmental impacts. Here, we evaluated the biological and physical microbiome interactions of three Atlantic salmon hatcheries (RAS n = 2, FT n = 1). Gill, skin, and digesta from six juvenile fish along with tank biofilms and water were sampled from tanks in each of the hatcheries (60 fish across 10 tanks) to assess the built environment and mucosal microbiota using 16S rRNA gene sequencing. The water and tank biofilm had more microbial richness than fish mucus, while skin and digesta from RAS fish had 2 times the richness of FT fish. Body sites each had unique microbiomes (P < 0.001) and were influenced by hatchery system type (P < 0.001), with RAS being more similar. A strong association between the tank and fish microbiome was observed. Water and tank biofilm richness was positively correlated with skin and digesta richness. Strikingly, the gill, skin, and digesta communities were more similar to that in the origin tank biofilm than those in all other experimental tanks, suggesting that the tank biofilm has a direct influence on fish-associated microbial communities. Lastly, microbial diversity and mucous cell density were positively associated with fish growth and length. The results from this study provide evidence for a link between the tank microbiome and the fish microbiome, with the skin microbiome as an important intermediate.IMPORTANCE Atlantic salmon, Salmo salar, is the most farmed marine fish worldwide, with an annual production of 2,248 million metric tons in 2016. Salmon hatcheries are increasingly changing from flowthrough toward recirculating aquaculture system (RAS) design to accommodate more control over production along with improved ... |
author2 |
Liu, Shuang-Jiang |
format |
Article in Journal/Newspaper |
author |
Minich, Jeremiah J Poore, Greg D Jantawongsri, Khattapan Johnston, Colin Bowie, Kate Bowman, John Knight, Rob Nowak, Barbara Allen, Eric E |
author_facet |
Minich, Jeremiah J Poore, Greg D Jantawongsri, Khattapan Johnston, Colin Bowie, Kate Bowman, John Knight, Rob Nowak, Barbara Allen, Eric E |
author_sort |
Minich, Jeremiah J |
title |
Microbial Ecology of Atlantic Salmon (Salmo salar) Hatcheries: Impacts of the Built Environment on Fish Mucosal Microbiota. |
title_short |
Microbial Ecology of Atlantic Salmon (Salmo salar) Hatcheries: Impacts of the Built Environment on Fish Mucosal Microbiota. |
title_full |
Microbial Ecology of Atlantic Salmon (Salmo salar) Hatcheries: Impacts of the Built Environment on Fish Mucosal Microbiota. |
title_fullStr |
Microbial Ecology of Atlantic Salmon (Salmo salar) Hatcheries: Impacts of the Built Environment on Fish Mucosal Microbiota. |
title_full_unstemmed |
Microbial Ecology of Atlantic Salmon (Salmo salar) Hatcheries: Impacts of the Built Environment on Fish Mucosal Microbiota. |
title_sort |
microbial ecology of atlantic salmon (salmo salar) hatcheries: impacts of the built environment on fish mucosal microbiota. |
publisher |
eScholarship, University of California |
publishDate |
2020 |
url |
https://escholarship.org/uc/item/19x3q61w |
op_coverage |
e00411 - e00420 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Applied and Environmental Microbiology, vol 86, iss 12 |
op_relation |
qt19x3q61w https://escholarship.org/uc/item/19x3q61w |
op_rights |
public |
_version_ |
1776199204050305024 |