Upwelling‐level acidification and pH/pCO2 variability moderate effects of ocean acidification on brain gene expression in the temperate surfperch, Embiotoca jacksoni
Acidification-induced changes in neurological function have been documented in several tropical marine fishes. Here, we investigate whether similar patterns of neurological impacts are observed in a temperate Pacific fish that naturally experiences regular and often large shifts in environmental pH/...
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2022
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ftcdlib:oai:escholarship.org:ark:/13030/qt0fn6m7hx 2023-10-01T03:58:35+02:00 Upwelling‐level acidification and pH/pCO2 variability moderate effects of ocean acidification on brain gene expression in the temperate surfperch, Embiotoca jacksoni Toy, Jason A Kroeker, Kristy J Logan, Cheryl A Takeshita, Yuichiro Longo, Gary C Bernardi, Giacomo 4707 - 4725 2022-09-01 application/pdf https://escholarship.org/uc/item/0fn6m7hx unknown eScholarship, University of California qt0fn6m7hx https://escholarship.org/uc/item/0fn6m7hx public Molecular Ecology, vol 31, iss 18 Life Below Water Animals Brain Carbon Dioxide Fishes Gene Expression Hydrogen-Ion Concentration Oceans and Seas Perciformes Seawater climate change differential gene expression environmental variability embiotocidae global change RNA-seq Biological Sciences Evolutionary Biology article 2022 ftcdlib 2023-09-04T18:04:08Z Acidification-induced changes in neurological function have been documented in several tropical marine fishes. Here, we investigate whether similar patterns of neurological impacts are observed in a temperate Pacific fish that naturally experiences regular and often large shifts in environmental pH/pCO2 . In two laboratory experiments, we tested the effect of acidification, as well as pH/pCO2 variability, on gene expression in the brain tissue of a common temperate kelp forest/estuarine fish, Embiotoca jacksoni. Experiment 1 employed static pH treatments (target pH=7.85/7.30), while Experiment 2 incorporated two variable treatments that oscillated around corresponding static treatments with the same mean (target pH=7.85/7.70) in an eight-day cycle (amplitude ± 0.15). We found that patterns of global gene expression differed across pH level treatments. Additionally, we identified differential expression of specific genes and enrichment of specific gene sets (GSEA) in comparisons of static pH treatments and in comparisons of static and variable pH treatments of the same mean pH. Importantly, we found that pH/pCO2 variability decreased the number of differentially expressed genes detected between high and low pH treatments, and that interindividual variability in gene expression was greater in variable treatments than static treatments. These results provide important confirmation of neurological impacts of acidification in a temperate fish species and, critically, that natural environmental variability may mediate the impacts of ocean acidification. Article in Journal/Newspaper Ocean acidification University of California: eScholarship Pacific |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Life Below Water Animals Brain Carbon Dioxide Fishes Gene Expression Hydrogen-Ion Concentration Oceans and Seas Perciformes Seawater climate change differential gene expression environmental variability embiotocidae global change RNA-seq Biological Sciences Evolutionary Biology |
spellingShingle |
Life Below Water Animals Brain Carbon Dioxide Fishes Gene Expression Hydrogen-Ion Concentration Oceans and Seas Perciformes Seawater climate change differential gene expression environmental variability embiotocidae global change RNA-seq Biological Sciences Evolutionary Biology Toy, Jason A Kroeker, Kristy J Logan, Cheryl A Takeshita, Yuichiro Longo, Gary C Bernardi, Giacomo Upwelling‐level acidification and pH/pCO2 variability moderate effects of ocean acidification on brain gene expression in the temperate surfperch, Embiotoca jacksoni |
topic_facet |
Life Below Water Animals Brain Carbon Dioxide Fishes Gene Expression Hydrogen-Ion Concentration Oceans and Seas Perciformes Seawater climate change differential gene expression environmental variability embiotocidae global change RNA-seq Biological Sciences Evolutionary Biology |
description |
Acidification-induced changes in neurological function have been documented in several tropical marine fishes. Here, we investigate whether similar patterns of neurological impacts are observed in a temperate Pacific fish that naturally experiences regular and often large shifts in environmental pH/pCO2 . In two laboratory experiments, we tested the effect of acidification, as well as pH/pCO2 variability, on gene expression in the brain tissue of a common temperate kelp forest/estuarine fish, Embiotoca jacksoni. Experiment 1 employed static pH treatments (target pH=7.85/7.30), while Experiment 2 incorporated two variable treatments that oscillated around corresponding static treatments with the same mean (target pH=7.85/7.70) in an eight-day cycle (amplitude ± 0.15). We found that patterns of global gene expression differed across pH level treatments. Additionally, we identified differential expression of specific genes and enrichment of specific gene sets (GSEA) in comparisons of static pH treatments and in comparisons of static and variable pH treatments of the same mean pH. Importantly, we found that pH/pCO2 variability decreased the number of differentially expressed genes detected between high and low pH treatments, and that interindividual variability in gene expression was greater in variable treatments than static treatments. These results provide important confirmation of neurological impacts of acidification in a temperate fish species and, critically, that natural environmental variability may mediate the impacts of ocean acidification. |
format |
Article in Journal/Newspaper |
author |
Toy, Jason A Kroeker, Kristy J Logan, Cheryl A Takeshita, Yuichiro Longo, Gary C Bernardi, Giacomo |
author_facet |
Toy, Jason A Kroeker, Kristy J Logan, Cheryl A Takeshita, Yuichiro Longo, Gary C Bernardi, Giacomo |
author_sort |
Toy, Jason A |
title |
Upwelling‐level acidification and pH/pCO2 variability moderate effects of ocean acidification on brain gene expression in the temperate surfperch, Embiotoca jacksoni |
title_short |
Upwelling‐level acidification and pH/pCO2 variability moderate effects of ocean acidification on brain gene expression in the temperate surfperch, Embiotoca jacksoni |
title_full |
Upwelling‐level acidification and pH/pCO2 variability moderate effects of ocean acidification on brain gene expression in the temperate surfperch, Embiotoca jacksoni |
title_fullStr |
Upwelling‐level acidification and pH/pCO2 variability moderate effects of ocean acidification on brain gene expression in the temperate surfperch, Embiotoca jacksoni |
title_full_unstemmed |
Upwelling‐level acidification and pH/pCO2 variability moderate effects of ocean acidification on brain gene expression in the temperate surfperch, Embiotoca jacksoni |
title_sort |
upwelling‐level acidification and ph/pco2 variability moderate effects of ocean acidification on brain gene expression in the temperate surfperch, embiotoca jacksoni |
publisher |
eScholarship, University of California |
publishDate |
2022 |
url |
https://escholarship.org/uc/item/0fn6m7hx |
op_coverage |
4707 - 4725 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Molecular Ecology, vol 31, iss 18 |
op_relation |
qt0fn6m7hx https://escholarship.org/uc/item/0fn6m7hx |
op_rights |
public |
_version_ |
1778531444920942592 |