Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost
Permafrost underlies a large portion of the land in the Northern Hemisphere. It is proposed to be an extreme habitat and home for cold-adaptive microbial communities. Upon thaw permafrost is predicted to exacerbate increasing global temperature trend, where awakening microbes decompose millennia old...
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ftcdlib:oai:escholarship.org:ark:/13030/qt053022qx 2024-01-14T10:09:48+01:00 Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost Xue, Yaxin Jonassen, Inge Øvreås, Lise Taş, Neslihan fiaa057 2020-05-01 application/pdf https://escholarship.org/uc/item/053022qx unknown eScholarship, University of California qt053022qx https://escholarship.org/uc/item/053022qx public FEMS Microbiology Ecology, vol 96, iss 5 Microbiology Biological Sciences Ecology Human Genome Genetics Metagenome Norway Permafrost Soil Soil Microbiology Svalbard microbiome metagenome-assembled genomes aerobic metabolism Environmental Sciences Medical and Health Sciences article 2020 ftcdlib 2023-12-18T19:07:19Z Permafrost underlies a large portion of the land in the Northern Hemisphere. It is proposed to be an extreme habitat and home for cold-adaptive microbial communities. Upon thaw permafrost is predicted to exacerbate increasing global temperature trend, where awakening microbes decompose millennia old carbon stocks. Yet our knowledge on composition, functional potential and variance of permafrost microbiome remains limited. In this study, we conducted a deep comparative metagenomic analysis through a 2 m permafrost core from Svalbard, Norway to determine key permafrost microbiome in this climate sensitive island ecosystem. To do so, we developed comparative metagenomics methods on metagenomic-assembled genomes (MAG). We found that community composition in Svalbard soil horizons shifted markedly with depth: the dominant phylum switched from Acidobacteria and Proteobacteria in top soils (active layer) to Actinobacteria, Bacteroidetes, Chloroflexi and Proteobacteria in permafrost layers. Key metabolic potential propagated through permafrost depths revealed aerobic respiration and soil organic matter decomposition as key metabolic traits. We also found that Svalbard MAGs were enriched in genes involved in regulation of ammonium, sulfur and phosphate. Here, we provide a new perspective on how permafrost microbiome is shaped to acquire resources in competitive and limited resource conditions of deep Svalbard soils. Article in Journal/Newspaper permafrost Svalbard University of California: eScholarship Norway Svalbard |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Microbiology Biological Sciences Ecology Human Genome Genetics Metagenome Norway Permafrost Soil Soil Microbiology Svalbard microbiome metagenome-assembled genomes aerobic metabolism Environmental Sciences Medical and Health Sciences |
spellingShingle |
Microbiology Biological Sciences Ecology Human Genome Genetics Metagenome Norway Permafrost Soil Soil Microbiology Svalbard microbiome metagenome-assembled genomes aerobic metabolism Environmental Sciences Medical and Health Sciences Xue, Yaxin Jonassen, Inge Øvreås, Lise Taş, Neslihan Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
topic_facet |
Microbiology Biological Sciences Ecology Human Genome Genetics Metagenome Norway Permafrost Soil Soil Microbiology Svalbard microbiome metagenome-assembled genomes aerobic metabolism Environmental Sciences Medical and Health Sciences |
description |
Permafrost underlies a large portion of the land in the Northern Hemisphere. It is proposed to be an extreme habitat and home for cold-adaptive microbial communities. Upon thaw permafrost is predicted to exacerbate increasing global temperature trend, where awakening microbes decompose millennia old carbon stocks. Yet our knowledge on composition, functional potential and variance of permafrost microbiome remains limited. In this study, we conducted a deep comparative metagenomic analysis through a 2 m permafrost core from Svalbard, Norway to determine key permafrost microbiome in this climate sensitive island ecosystem. To do so, we developed comparative metagenomics methods on metagenomic-assembled genomes (MAG). We found that community composition in Svalbard soil horizons shifted markedly with depth: the dominant phylum switched from Acidobacteria and Proteobacteria in top soils (active layer) to Actinobacteria, Bacteroidetes, Chloroflexi and Proteobacteria in permafrost layers. Key metabolic potential propagated through permafrost depths revealed aerobic respiration and soil organic matter decomposition as key metabolic traits. We also found that Svalbard MAGs were enriched in genes involved in regulation of ammonium, sulfur and phosphate. Here, we provide a new perspective on how permafrost microbiome is shaped to acquire resources in competitive and limited resource conditions of deep Svalbard soils. |
format |
Article in Journal/Newspaper |
author |
Xue, Yaxin Jonassen, Inge Øvreås, Lise Taş, Neslihan |
author_facet |
Xue, Yaxin Jonassen, Inge Øvreås, Lise Taş, Neslihan |
author_sort |
Xue, Yaxin |
title |
Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
title_short |
Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
title_full |
Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
title_fullStr |
Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
title_full_unstemmed |
Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
title_sort |
metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in svalbard permafrost |
publisher |
eScholarship, University of California |
publishDate |
2020 |
url |
https://escholarship.org/uc/item/053022qx |
op_coverage |
fiaa057 |
geographic |
Norway Svalbard |
geographic_facet |
Norway Svalbard |
genre |
permafrost Svalbard |
genre_facet |
permafrost Svalbard |
op_source |
FEMS Microbiology Ecology, vol 96, iss 5 |
op_relation |
qt053022qx https://escholarship.org/uc/item/053022qx |
op_rights |
public |
_version_ |
1788064373064859648 |