Quantification of Cutaneous Ionocytes in Small Aquatic Organisms.
Aquatic organisms have specialized cells called ionocytes that regulate the ionic composition, osmolarity, and acid/base status of internal fluids. In small aquatic organisms such as fishes in their early life stages, ionocytes are typically found on the cutaneous surface and their abundance can cha...
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ftcdlib:oai:escholarship.org/ark:/13030/qt9dk2g3pp 2023-05-15T17:51:20+02:00 Quantification of Cutaneous Ionocytes in Small Aquatic Organisms. Kwan, Garfield T Finnerty, Shane H Wegner, Nicholas C Tresguerres, Martin e3227 2019-05-05 https://escholarship.org/uc/item/9dk2g3pp unknown eScholarship, University of California qt9dk2g3pp https://escholarship.org/uc/item/9dk2g3pp public Bio-protocol, vol 9, iss 9 ATPase Chloride cell Larvae Mitochondrion-rich cell Ocean acidification Osmoregulation Skin pH regulation article 2019 ftcdlib 2021-03-28T08:19:37Z Aquatic organisms have specialized cells called ionocytes that regulate the ionic composition, osmolarity, and acid/base status of internal fluids. In small aquatic organisms such as fishes in their early life stages, ionocytes are typically found on the cutaneous surface and their abundance can change to help cope with various metabolic and environmental factors. Ionocytes profusely express ATPase enzymes, most notably Na+/K+ ATPase, which can be identified by immunohistochemistry. However, quantification of cutaneous ionocytes is not trivial due to the limited camera's focal plane and the microscope's field-of-view. This protocol describes a technique to consistently and reliably identify, image, and measure the relative surface area covered by cutaneous ionocytes through software-mediated focus-stacking and photo-stitching-thereby allowing the quantification of cutaneous ionocyte area as a proxy for ion transporting capacity across the skin. Because ionocytes are essential for regulating ionic composition, osmolarity, and acid/base status of internal fluids, this technique is useful for studying physiological mechanisms used by fish larvae and other small aquatic organisms during development and in response to environmental stress. Article in Journal/Newspaper Ocean acidification University of California: eScholarship |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
ATPase Chloride cell Larvae Mitochondrion-rich cell Ocean acidification Osmoregulation Skin pH regulation |
spellingShingle |
ATPase Chloride cell Larvae Mitochondrion-rich cell Ocean acidification Osmoregulation Skin pH regulation Kwan, Garfield T Finnerty, Shane H Wegner, Nicholas C Tresguerres, Martin Quantification of Cutaneous Ionocytes in Small Aquatic Organisms. |
topic_facet |
ATPase Chloride cell Larvae Mitochondrion-rich cell Ocean acidification Osmoregulation Skin pH regulation |
description |
Aquatic organisms have specialized cells called ionocytes that regulate the ionic composition, osmolarity, and acid/base status of internal fluids. In small aquatic organisms such as fishes in their early life stages, ionocytes are typically found on the cutaneous surface and their abundance can change to help cope with various metabolic and environmental factors. Ionocytes profusely express ATPase enzymes, most notably Na+/K+ ATPase, which can be identified by immunohistochemistry. However, quantification of cutaneous ionocytes is not trivial due to the limited camera's focal plane and the microscope's field-of-view. This protocol describes a technique to consistently and reliably identify, image, and measure the relative surface area covered by cutaneous ionocytes through software-mediated focus-stacking and photo-stitching-thereby allowing the quantification of cutaneous ionocyte area as a proxy for ion transporting capacity across the skin. Because ionocytes are essential for regulating ionic composition, osmolarity, and acid/base status of internal fluids, this technique is useful for studying physiological mechanisms used by fish larvae and other small aquatic organisms during development and in response to environmental stress. |
format |
Article in Journal/Newspaper |
author |
Kwan, Garfield T Finnerty, Shane H Wegner, Nicholas C Tresguerres, Martin |
author_facet |
Kwan, Garfield T Finnerty, Shane H Wegner, Nicholas C Tresguerres, Martin |
author_sort |
Kwan, Garfield T |
title |
Quantification of Cutaneous Ionocytes in Small Aquatic Organisms. |
title_short |
Quantification of Cutaneous Ionocytes in Small Aquatic Organisms. |
title_full |
Quantification of Cutaneous Ionocytes in Small Aquatic Organisms. |
title_fullStr |
Quantification of Cutaneous Ionocytes in Small Aquatic Organisms. |
title_full_unstemmed |
Quantification of Cutaneous Ionocytes in Small Aquatic Organisms. |
title_sort |
quantification of cutaneous ionocytes in small aquatic organisms. |
publisher |
eScholarship, University of California |
publishDate |
2019 |
url |
https://escholarship.org/uc/item/9dk2g3pp |
op_coverage |
e3227 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Bio-protocol, vol 9, iss 9 |
op_relation |
qt9dk2g3pp https://escholarship.org/uc/item/9dk2g3pp |
op_rights |
public |
_version_ |
1766158446248329216 |