Long-Term Warming in Alaska Enlarges the Diazotrophic Community in Deep Soils.
Tundra ecosystems are typically carbon (C) rich but nitrogen (N) limited. Since biological N2 fixation is the major source of biologically available N, the soil N2-fixing (i.e., diazotrophic) community serves as an essential N supplier to the tundra ecosystem. Recent climate warming has induced deep...
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ftcdlib:oai:escholarship.org/ark:/13030/qt92q2d4kd 2023-05-15T17:57:51+02:00 Long-Term Warming in Alaska Enlarges the Diazotrophic Community in Deep Soils. Feng, Jiajie Penton, C Ryan He, Zhili Van Nostrand, Joy D Yuan, Mengting M Wu, Liyou Wang, Cong Qin, Yujia Shi, Zhou J Guo, Xue Schuur, Edward AG Luo, Yiqi Bracho, Rosvel Konstantinidis, Konstantinos T Cole, James R Tiedje, James M Yang, Yunfeng Zhou, Jizhong e02521 - e02518 2019-02-26 application/pdf https://escholarship.org/uc/item/92q2d4kd unknown eScholarship, University of California qt92q2d4kd https://escholarship.org/uc/item/92q2d4kd public mBio, vol 10, iss 1 Oxidoreductases Microarray Analysis Soil Microbiology Nitrogen Fixation Alaska Metagenomics Global Warming Biota Plant Development Tundra climate warming diazotrophs gene sequencing Microbiology article 2019 ftcdlib 2021-11-08T18:16:08Z Tundra ecosystems are typically carbon (C) rich but nitrogen (N) limited. Since biological N2 fixation is the major source of biologically available N, the soil N2-fixing (i.e., diazotrophic) community serves as an essential N supplier to the tundra ecosystem. Recent climate warming has induced deeper permafrost thaw and adversely affected C sequestration, which is modulated by N availability. Therefore, it is crucial to examine the responses of diazotrophic communities to warming across the depths of tundra soils. Herein, we carried out one of the deepest sequencing efforts of nitrogenase gene (nifH) to investigate how 5 years of experimental winter warming affects Alaskan soil diazotrophic community composition and abundance spanning both the organic and mineral layers. Although soil depth had a stronger influence on diazotrophic community composition than warming, warming significantly (P < 0.05) enhanced diazotrophic abundance by 86.3% and aboveground plant biomass by 25.2%. Diazotrophic composition in the middle and lower organic layers, detected by nifH sequencing and a microarray-based tool (GeoChip), was markedly altered, with an increase of α-diversity. Changes in diazotrophic abundance and composition significantly correlated with soil moisture, soil thaw duration, and plant biomass, as shown by structural equation modeling analyses. Therefore, more abundant diazotrophic communities induced by warming may potentially serve as an important mechanism for supplementing biologically available N in this tundra ecosystem.IMPORTANCE With the likelihood that changes in global climate will adversely affect the soil C reservoir in the northern circumpolar permafrost zone, an understanding of the potential role of diazotrophic communities in enhancing biological N2 fixation, which constrains both plant production and microbial decomposition in tundra soils, is important in elucidating the responses of soil microbial communities to global climate change. A recent study showed that the composition of the diazotrophic community in a tundra soil exhibited no change under a short-term (1.5-year) winter warming experiment. However, it remains crucial to examine whether the lack of diazotrophic community responses to warming is persistent over a longer time period as a possibly important mechanism in stabilizing tundra soil C. Through a detailed characterization of the effects of winter warming on diazotrophic communities, we showed that a long-term (5-year) winter warming substantially enhanced diazotrophic abundance and altered community composition, though soil depth had a stronger influence on diazotrophic community composition than warming. These changes were best explained by changes in soil moisture, soil thaw duration, and plant biomass. These results provide crucial insights into the potential factors that may impact future C and N availability in tundra regions. Article in Journal/Newspaper permafrost Tundra Alaska University of California: eScholarship |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Oxidoreductases Microarray Analysis Soil Microbiology Nitrogen Fixation Alaska Metagenomics Global Warming Biota Plant Development Tundra climate warming diazotrophs gene sequencing Microbiology |
spellingShingle |
Oxidoreductases Microarray Analysis Soil Microbiology Nitrogen Fixation Alaska Metagenomics Global Warming Biota Plant Development Tundra climate warming diazotrophs gene sequencing Microbiology Feng, Jiajie Penton, C Ryan He, Zhili Van Nostrand, Joy D Yuan, Mengting M Wu, Liyou Wang, Cong Qin, Yujia Shi, Zhou J Guo, Xue Schuur, Edward AG Luo, Yiqi Bracho, Rosvel Konstantinidis, Konstantinos T Cole, James R Tiedje, James M Yang, Yunfeng Zhou, Jizhong Long-Term Warming in Alaska Enlarges the Diazotrophic Community in Deep Soils. |
topic_facet |
Oxidoreductases Microarray Analysis Soil Microbiology Nitrogen Fixation Alaska Metagenomics Global Warming Biota Plant Development Tundra climate warming diazotrophs gene sequencing Microbiology |
description |
Tundra ecosystems are typically carbon (C) rich but nitrogen (N) limited. Since biological N2 fixation is the major source of biologically available N, the soil N2-fixing (i.e., diazotrophic) community serves as an essential N supplier to the tundra ecosystem. Recent climate warming has induced deeper permafrost thaw and adversely affected C sequestration, which is modulated by N availability. Therefore, it is crucial to examine the responses of diazotrophic communities to warming across the depths of tundra soils. Herein, we carried out one of the deepest sequencing efforts of nitrogenase gene (nifH) to investigate how 5 years of experimental winter warming affects Alaskan soil diazotrophic community composition and abundance spanning both the organic and mineral layers. Although soil depth had a stronger influence on diazotrophic community composition than warming, warming significantly (P < 0.05) enhanced diazotrophic abundance by 86.3% and aboveground plant biomass by 25.2%. Diazotrophic composition in the middle and lower organic layers, detected by nifH sequencing and a microarray-based tool (GeoChip), was markedly altered, with an increase of α-diversity. Changes in diazotrophic abundance and composition significantly correlated with soil moisture, soil thaw duration, and plant biomass, as shown by structural equation modeling analyses. Therefore, more abundant diazotrophic communities induced by warming may potentially serve as an important mechanism for supplementing biologically available N in this tundra ecosystem.IMPORTANCE With the likelihood that changes in global climate will adversely affect the soil C reservoir in the northern circumpolar permafrost zone, an understanding of the potential role of diazotrophic communities in enhancing biological N2 fixation, which constrains both plant production and microbial decomposition in tundra soils, is important in elucidating the responses of soil microbial communities to global climate change. A recent study showed that the composition of the diazotrophic community in a tundra soil exhibited no change under a short-term (1.5-year) winter warming experiment. However, it remains crucial to examine whether the lack of diazotrophic community responses to warming is persistent over a longer time period as a possibly important mechanism in stabilizing tundra soil C. Through a detailed characterization of the effects of winter warming on diazotrophic communities, we showed that a long-term (5-year) winter warming substantially enhanced diazotrophic abundance and altered community composition, though soil depth had a stronger influence on diazotrophic community composition than warming. These changes were best explained by changes in soil moisture, soil thaw duration, and plant biomass. These results provide crucial insights into the potential factors that may impact future C and N availability in tundra regions. |
format |
Article in Journal/Newspaper |
author |
Feng, Jiajie Penton, C Ryan He, Zhili Van Nostrand, Joy D Yuan, Mengting M Wu, Liyou Wang, Cong Qin, Yujia Shi, Zhou J Guo, Xue Schuur, Edward AG Luo, Yiqi Bracho, Rosvel Konstantinidis, Konstantinos T Cole, James R Tiedje, James M Yang, Yunfeng Zhou, Jizhong |
author_facet |
Feng, Jiajie Penton, C Ryan He, Zhili Van Nostrand, Joy D Yuan, Mengting M Wu, Liyou Wang, Cong Qin, Yujia Shi, Zhou J Guo, Xue Schuur, Edward AG Luo, Yiqi Bracho, Rosvel Konstantinidis, Konstantinos T Cole, James R Tiedje, James M Yang, Yunfeng Zhou, Jizhong |
author_sort |
Feng, Jiajie |
title |
Long-Term Warming in Alaska Enlarges the Diazotrophic Community in Deep Soils. |
title_short |
Long-Term Warming in Alaska Enlarges the Diazotrophic Community in Deep Soils. |
title_full |
Long-Term Warming in Alaska Enlarges the Diazotrophic Community in Deep Soils. |
title_fullStr |
Long-Term Warming in Alaska Enlarges the Diazotrophic Community in Deep Soils. |
title_full_unstemmed |
Long-Term Warming in Alaska Enlarges the Diazotrophic Community in Deep Soils. |
title_sort |
long-term warming in alaska enlarges the diazotrophic community in deep soils. |
publisher |
eScholarship, University of California |
publishDate |
2019 |
url |
https://escholarship.org/uc/item/92q2d4kd |
op_coverage |
e02521 - e02518 |
genre |
permafrost Tundra Alaska |
genre_facet |
permafrost Tundra Alaska |
op_source |
mBio, vol 10, iss 1 |
op_relation |
qt92q2d4kd https://escholarship.org/uc/item/92q2d4kd |
op_rights |
public |
_version_ |
1766166363894710272 |