Metagenome-assembled genomes provide new insight into the microbial diversity of two thermal pools in Kamchatka, Russia.
Culture-independent methods have contributed substantially to our understanding of global microbial diversity. Recently developed algorithms to construct whole genomes from environmental samples have further refined, corrected and revolutionized understanding of the tree of life. Here, we assembled...
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ftcdlib:oai:escholarship.org/ark:/13030/qt8s59d6dn 2023-05-15T16:58:41+02:00 Metagenome-assembled genomes provide new insight into the microbial diversity of two thermal pools in Kamchatka, Russia. Wilkins, Laetitia Ettinger, Cassandra Jospin, Guillaume Eisen, Jonathan 2019-02-28 application/pdf https://escholarship.org/uc/item/8s59d6dn unknown eScholarship, University of California qt8s59d6dn https://escholarship.org/uc/item/8s59d6dn public Scientific Reports, vol 9, iss 1 article 2019 ftcdlib 2020-06-06T07:53:33Z Culture-independent methods have contributed substantially to our understanding of global microbial diversity. Recently developed algorithms to construct whole genomes from environmental samples have further refined, corrected and revolutionized understanding of the tree of life. Here, we assembled draft metagenome-assembled genomes (MAGs) from environmental DNA extracted from two hot springs within an active volcanic ecosystem on the Kamchatka peninsula, Russia. This hydrothermal system has been intensively studied previously with regard to geochemistry, chemoautotrophy, microbial isolation, and microbial diversity. We assembled genomes of bacteria and archaea using DNA that had previously been characterized via 16S rRNA gene clone libraries. We recovered 36 MAGs, 29 of medium to high quality, and inferred their placement in a phylogenetic tree consisting of 3,240 publicly available microbial genomes. We highlight MAGs that were taxonomically assigned to groups previously underrepresented in available genome data. This includes several archaea (Korarchaeota, Bathyarchaeota and Aciduliprofundum) and one potentially new species within the bacterial genus Sulfurihydrogenibium. Putative functions in both pools were compared and are discussed in the context of their diverging geochemistry. This study adds comprehensive information about phylogenetic diversity and functional potential within two hot springs in the caldera of Kamchatka. Article in Journal/Newspaper Kamchatka Kamchatka Peninsula University of California: eScholarship Kamchatka Peninsula ENVELOPE(160.000,160.000,56.000,56.000) |
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Open Polar |
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University of California: eScholarship |
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description |
Culture-independent methods have contributed substantially to our understanding of global microbial diversity. Recently developed algorithms to construct whole genomes from environmental samples have further refined, corrected and revolutionized understanding of the tree of life. Here, we assembled draft metagenome-assembled genomes (MAGs) from environmental DNA extracted from two hot springs within an active volcanic ecosystem on the Kamchatka peninsula, Russia. This hydrothermal system has been intensively studied previously with regard to geochemistry, chemoautotrophy, microbial isolation, and microbial diversity. We assembled genomes of bacteria and archaea using DNA that had previously been characterized via 16S rRNA gene clone libraries. We recovered 36 MAGs, 29 of medium to high quality, and inferred their placement in a phylogenetic tree consisting of 3,240 publicly available microbial genomes. We highlight MAGs that were taxonomically assigned to groups previously underrepresented in available genome data. This includes several archaea (Korarchaeota, Bathyarchaeota and Aciduliprofundum) and one potentially new species within the bacterial genus Sulfurihydrogenibium. Putative functions in both pools were compared and are discussed in the context of their diverging geochemistry. This study adds comprehensive information about phylogenetic diversity and functional potential within two hot springs in the caldera of Kamchatka. |
format |
Article in Journal/Newspaper |
author |
Wilkins, Laetitia Ettinger, Cassandra Jospin, Guillaume Eisen, Jonathan |
spellingShingle |
Wilkins, Laetitia Ettinger, Cassandra Jospin, Guillaume Eisen, Jonathan Metagenome-assembled genomes provide new insight into the microbial diversity of two thermal pools in Kamchatka, Russia. |
author_facet |
Wilkins, Laetitia Ettinger, Cassandra Jospin, Guillaume Eisen, Jonathan |
author_sort |
Wilkins, Laetitia |
title |
Metagenome-assembled genomes provide new insight into the microbial diversity of two thermal pools in Kamchatka, Russia. |
title_short |
Metagenome-assembled genomes provide new insight into the microbial diversity of two thermal pools in Kamchatka, Russia. |
title_full |
Metagenome-assembled genomes provide new insight into the microbial diversity of two thermal pools in Kamchatka, Russia. |
title_fullStr |
Metagenome-assembled genomes provide new insight into the microbial diversity of two thermal pools in Kamchatka, Russia. |
title_full_unstemmed |
Metagenome-assembled genomes provide new insight into the microbial diversity of two thermal pools in Kamchatka, Russia. |
title_sort |
metagenome-assembled genomes provide new insight into the microbial diversity of two thermal pools in kamchatka, russia. |
publisher |
eScholarship, University of California |
publishDate |
2019 |
url |
https://escholarship.org/uc/item/8s59d6dn |
long_lat |
ENVELOPE(160.000,160.000,56.000,56.000) |
geographic |
Kamchatka Peninsula |
geographic_facet |
Kamchatka Peninsula |
genre |
Kamchatka Kamchatka Peninsula |
genre_facet |
Kamchatka Kamchatka Peninsula |
op_source |
Scientific Reports, vol 9, iss 1 |
op_relation |
qt8s59d6dn https://escholarship.org/uc/item/8s59d6dn |
op_rights |
public |
_version_ |
1766050764192481280 |