Contribution of ammonia oxidation to chemoautotrophy in Antarctic coastal waters.
There are few measurements of nitrification in polar regions, yet geochemical evidence suggests that it is significant, and chemoautotrophy supported by nitrification has been suggested as an important contribution to prokaryotic production during the polar winter. This study reports seasonal ammoni...
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ftcdlib:oai:escholarship.org/ark:/13030/qt8kw9m35p 2023-05-15T14:00:50+02:00 Contribution of ammonia oxidation to chemoautotrophy in Antarctic coastal waters. Tolar, Bradley B Ross, Meredith J Wallsgrove, Natalie J Liu, Qian Aluwihare, Lihini I Popp, Brian N Hollibaugh, James T 2605 - 2619 2016-11-01 application/pdf https://escholarship.org/uc/item/8kw9m35p unknown eScholarship, University of California qt8kw9m35p https://escholarship.org/uc/item/8kw9m35p public The ISME journal, vol 10, iss 11 Archaea Ammonia Oxidoreductases Seasons Seawater Phylogeny Oxidation-Reduction Genes Archaeal Antarctic Regions Oceans and Seas Chemoautotrophic Growth Nitrification Microbiology Environmental Sciences Biological Sciences Technology article 2016 ftcdlib 2020-06-06T07:52:50Z There are few measurements of nitrification in polar regions, yet geochemical evidence suggests that it is significant, and chemoautotrophy supported by nitrification has been suggested as an important contribution to prokaryotic production during the polar winter. This study reports seasonal ammonia oxidation (AO) rates, gene and transcript abundance in continental shelf waters west of the Antarctic Peninsula, where Thaumarchaeota strongly dominate populations of ammonia-oxidizing organisms. Higher AO rates were observed in the late winter surface mixed layer compared with the same water mass sampled during summer (mean±s.e.: 62±16 versus 13±2.8 nm per day, t-test P<0.0005). AO rates in the circumpolar deep water did not differ between seasons (21±5.7 versus 24±6.6 nm per day; P=0.83), despite 5- to 20-fold greater Thaumarchaeota abundance during summer. AO rates correlated with concentrations of Archaea ammonia monooxygenase (amoA) genes during summer, but not with concentrations of Archaea amoA transcripts, or with ratios of Archaea amoA transcripts per gene, or with concentrations of Betaproteobacterial amoA genes or transcripts. The AO rates we report (<0.1-220 nm per day) are ~10-fold greater than reported previously for Antarctic waters and suggest that inclusion of Antarctic coastal waters in global estimates of oceanic nitrification could increase global rate estimates by ~9%. Chemoautotrophic carbon fixation supported by AO was 3-6% of annualized phytoplankton primary production and production of Thaumarchaeota biomass supported by AO could account for ~9% of the bacterioplankton production measured in winter. Growth rates of thaumarchaeote populations inferred from AO rates averaged 0.3 per day and ranged from 0.01 to 2.1 per day. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula University of California: eScholarship Antarctic The Antarctic Antarctic Peninsula |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Archaea Ammonia Oxidoreductases Seasons Seawater Phylogeny Oxidation-Reduction Genes Archaeal Antarctic Regions Oceans and Seas Chemoautotrophic Growth Nitrification Microbiology Environmental Sciences Biological Sciences Technology |
spellingShingle |
Archaea Ammonia Oxidoreductases Seasons Seawater Phylogeny Oxidation-Reduction Genes Archaeal Antarctic Regions Oceans and Seas Chemoautotrophic Growth Nitrification Microbiology Environmental Sciences Biological Sciences Technology Tolar, Bradley B Ross, Meredith J Wallsgrove, Natalie J Liu, Qian Aluwihare, Lihini I Popp, Brian N Hollibaugh, James T Contribution of ammonia oxidation to chemoautotrophy in Antarctic coastal waters. |
topic_facet |
Archaea Ammonia Oxidoreductases Seasons Seawater Phylogeny Oxidation-Reduction Genes Archaeal Antarctic Regions Oceans and Seas Chemoautotrophic Growth Nitrification Microbiology Environmental Sciences Biological Sciences Technology |
description |
There are few measurements of nitrification in polar regions, yet geochemical evidence suggests that it is significant, and chemoautotrophy supported by nitrification has been suggested as an important contribution to prokaryotic production during the polar winter. This study reports seasonal ammonia oxidation (AO) rates, gene and transcript abundance in continental shelf waters west of the Antarctic Peninsula, where Thaumarchaeota strongly dominate populations of ammonia-oxidizing organisms. Higher AO rates were observed in the late winter surface mixed layer compared with the same water mass sampled during summer (mean±s.e.: 62±16 versus 13±2.8 nm per day, t-test P<0.0005). AO rates in the circumpolar deep water did not differ between seasons (21±5.7 versus 24±6.6 nm per day; P=0.83), despite 5- to 20-fold greater Thaumarchaeota abundance during summer. AO rates correlated with concentrations of Archaea ammonia monooxygenase (amoA) genes during summer, but not with concentrations of Archaea amoA transcripts, or with ratios of Archaea amoA transcripts per gene, or with concentrations of Betaproteobacterial amoA genes or transcripts. The AO rates we report (<0.1-220 nm per day) are ~10-fold greater than reported previously for Antarctic waters and suggest that inclusion of Antarctic coastal waters in global estimates of oceanic nitrification could increase global rate estimates by ~9%. Chemoautotrophic carbon fixation supported by AO was 3-6% of annualized phytoplankton primary production and production of Thaumarchaeota biomass supported by AO could account for ~9% of the bacterioplankton production measured in winter. Growth rates of thaumarchaeote populations inferred from AO rates averaged 0.3 per day and ranged from 0.01 to 2.1 per day. |
format |
Article in Journal/Newspaper |
author |
Tolar, Bradley B Ross, Meredith J Wallsgrove, Natalie J Liu, Qian Aluwihare, Lihini I Popp, Brian N Hollibaugh, James T |
author_facet |
Tolar, Bradley B Ross, Meredith J Wallsgrove, Natalie J Liu, Qian Aluwihare, Lihini I Popp, Brian N Hollibaugh, James T |
author_sort |
Tolar, Bradley B |
title |
Contribution of ammonia oxidation to chemoautotrophy in Antarctic coastal waters. |
title_short |
Contribution of ammonia oxidation to chemoautotrophy in Antarctic coastal waters. |
title_full |
Contribution of ammonia oxidation to chemoautotrophy in Antarctic coastal waters. |
title_fullStr |
Contribution of ammonia oxidation to chemoautotrophy in Antarctic coastal waters. |
title_full_unstemmed |
Contribution of ammonia oxidation to chemoautotrophy in Antarctic coastal waters. |
title_sort |
contribution of ammonia oxidation to chemoautotrophy in antarctic coastal waters. |
publisher |
eScholarship, University of California |
publishDate |
2016 |
url |
https://escholarship.org/uc/item/8kw9m35p |
op_coverage |
2605 - 2619 |
geographic |
Antarctic The Antarctic Antarctic Peninsula |
geographic_facet |
Antarctic The Antarctic Antarctic Peninsula |
genre |
Antarc* Antarctic Antarctic Peninsula |
genre_facet |
Antarc* Antarctic Antarctic Peninsula |
op_source |
The ISME journal, vol 10, iss 11 |
op_relation |
qt8kw9m35p https://escholarship.org/uc/item/8kw9m35p |
op_rights |
public |
_version_ |
1766270172611477504 |