Oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal.
The prolonged, postweaning fast of northern elephant seal (Mirounga angustirostris) pups is characterized by a reliance on lipid metabolism and reversible, fasting-induced insulin resistance, providing a unique model to examine the effects of insulin on lipid metabolism. We have previously shown tha...
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ftcdlib:oai:escholarship.org/ark:/13030/qt5cv9b3vb 2023-05-15T16:05:12+02:00 Oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal. Wright, Dana N Katundu, Kondwani GH Viscarra, Jose A Crocker, Daniel E Newman, John W La Frano, Michael R Ortiz, Rudy M R537 - R546 2021-10-01 application/pdf https://escholarship.org/uc/item/5cv9b3vb unknown eScholarship, University of California qt5cv9b3vb https://escholarship.org/uc/item/5cv9b3vb public American journal of physiology. Regulatory, integrative and comparative physiology, vol 321, iss 4 Animals Seals Earless Insulin Resistance Insulin Hypoglycemic Agents Fasting Infusions Parenteral Lipid Metabolism Oxylipins Biomarkers lipid mediators lipids Nutrition Diabetes Metabolic and endocrine Biological Sciences Medical and Health Sciences Physiology article 2021 ftcdlib 2021-11-15T18:17:03Z The prolonged, postweaning fast of northern elephant seal (Mirounga angustirostris) pups is characterized by a reliance on lipid metabolism and reversible, fasting-induced insulin resistance, providing a unique model to examine the effects of insulin on lipid metabolism. We have previously shown that acute insulin infusion induced a shift in fatty acid metabolism dependent on fasting duration. This study complements the previous study by examining the effects of fasting duration and insulin infusion on circulating levels of oxylipins, bioactive metabolites derived from the oxygenation of polyunsaturated fatty acids. Northern elephant seal pups were studied at two postweaning periods (n = 5/period): early fasting (1-2 wk postweaning; 127 ± 1 kg) and late fasting (6-7 wk postweaning; 93 ± 4 kg). Different cohorts of pups were weighed, sedated, and infused with 65 mU/kg of insulin. Plasma was collected prior to infusion (T0) and at 10, 30, 60, and 120 min postinfusion. A profile of ∼80 oxylipins was analyzed by UPLC-ESI-MS/MS. Nine oxylipins changed between early and late fasting and eight were altered in response to insulin infusion. Fasting decreased prostaglandin F2α (PGF2α) and increased 14,15-dihydroxyicosatrienoic acid (14,15-DiHETrE), 20-hydroxyeicosatetraenoic acid (20-HETE), and 4-hydroxy-docosahexaenoic acid (4-HDoHE) (P < 0.03) in T0 samples, whereas insulin infusion resulted in an inverse change in area-under-the-curve (AUC) levels in these same metabolites (P < 0.05). In addition, 12-12-hydroperoxyeicosatetraenoic acid (HpETE) and 12-HETE decreased with fasting and insulin infusion, respectively (P < 0.04). The oxylipins altered during fasting and in response to insulin infusion may contribute to the manifestation of insulin resistance and participate in the metabolic regulation of associated cellular processes. Article in Journal/Newspaper Elephant Seal University of California: eScholarship |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Animals Seals Earless Insulin Resistance Insulin Hypoglycemic Agents Fasting Infusions Parenteral Lipid Metabolism Oxylipins Biomarkers lipid mediators lipids Nutrition Diabetes Metabolic and endocrine Biological Sciences Medical and Health Sciences Physiology |
spellingShingle |
Animals Seals Earless Insulin Resistance Insulin Hypoglycemic Agents Fasting Infusions Parenteral Lipid Metabolism Oxylipins Biomarkers lipid mediators lipids Nutrition Diabetes Metabolic and endocrine Biological Sciences Medical and Health Sciences Physiology Wright, Dana N Katundu, Kondwani GH Viscarra, Jose A Crocker, Daniel E Newman, John W La Frano, Michael R Ortiz, Rudy M Oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal. |
topic_facet |
Animals Seals Earless Insulin Resistance Insulin Hypoglycemic Agents Fasting Infusions Parenteral Lipid Metabolism Oxylipins Biomarkers lipid mediators lipids Nutrition Diabetes Metabolic and endocrine Biological Sciences Medical and Health Sciences Physiology |
description |
The prolonged, postweaning fast of northern elephant seal (Mirounga angustirostris) pups is characterized by a reliance on lipid metabolism and reversible, fasting-induced insulin resistance, providing a unique model to examine the effects of insulin on lipid metabolism. We have previously shown that acute insulin infusion induced a shift in fatty acid metabolism dependent on fasting duration. This study complements the previous study by examining the effects of fasting duration and insulin infusion on circulating levels of oxylipins, bioactive metabolites derived from the oxygenation of polyunsaturated fatty acids. Northern elephant seal pups were studied at two postweaning periods (n = 5/period): early fasting (1-2 wk postweaning; 127 ± 1 kg) and late fasting (6-7 wk postweaning; 93 ± 4 kg). Different cohorts of pups were weighed, sedated, and infused with 65 mU/kg of insulin. Plasma was collected prior to infusion (T0) and at 10, 30, 60, and 120 min postinfusion. A profile of ∼80 oxylipins was analyzed by UPLC-ESI-MS/MS. Nine oxylipins changed between early and late fasting and eight were altered in response to insulin infusion. Fasting decreased prostaglandin F2α (PGF2α) and increased 14,15-dihydroxyicosatrienoic acid (14,15-DiHETrE), 20-hydroxyeicosatetraenoic acid (20-HETE), and 4-hydroxy-docosahexaenoic acid (4-HDoHE) (P < 0.03) in T0 samples, whereas insulin infusion resulted in an inverse change in area-under-the-curve (AUC) levels in these same metabolites (P < 0.05). In addition, 12-12-hydroperoxyeicosatetraenoic acid (HpETE) and 12-HETE decreased with fasting and insulin infusion, respectively (P < 0.04). The oxylipins altered during fasting and in response to insulin infusion may contribute to the manifestation of insulin resistance and participate in the metabolic regulation of associated cellular processes. |
format |
Article in Journal/Newspaper |
author |
Wright, Dana N Katundu, Kondwani GH Viscarra, Jose A Crocker, Daniel E Newman, John W La Frano, Michael R Ortiz, Rudy M |
author_facet |
Wright, Dana N Katundu, Kondwani GH Viscarra, Jose A Crocker, Daniel E Newman, John W La Frano, Michael R Ortiz, Rudy M |
author_sort |
Wright, Dana N |
title |
Oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal. |
title_short |
Oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal. |
title_full |
Oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal. |
title_fullStr |
Oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal. |
title_full_unstemmed |
Oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal. |
title_sort |
oxylipin responses to fasting and insulin infusion in a large mammalian model of fasting-induced insulin resistance, the northern elephant seal. |
publisher |
eScholarship, University of California |
publishDate |
2021 |
url |
https://escholarship.org/uc/item/5cv9b3vb |
op_coverage |
R537 - R546 |
genre |
Elephant Seal |
genre_facet |
Elephant Seal |
op_source |
American journal of physiology. Regulatory, integrative and comparative physiology, vol 321, iss 4 |
op_relation |
qt5cv9b3vb https://escholarship.org/uc/item/5cv9b3vb |
op_rights |
public |
_version_ |
1766401040708534272 |