Genomic Characterization of the Evolutionary Potential of the Sea Urchin Strongylocentrotus droebachiensis Facing Ocean Acidification.
Ocean acidification (OA) is increasing due to anthropogenic CO2 emissions and poses a threat to marine species and communities worldwide. To better project the effects of acidification on organisms' health and persistence, an understanding is needed of the 1) mechanisms underlying developmental...
Main Authors: | , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
eScholarship, University of California
2016
|
Subjects: | |
Online Access: | https://escholarship.org/uc/item/02d5f9g3 |
id |
ftcdlib:oai:escholarship.org/ark:/13030/qt02d5f9g3 |
---|---|
record_format |
openpolar |
spelling |
ftcdlib:oai:escholarship.org/ark:/13030/qt02d5f9g3 2023-05-15T17:50:44+02:00 Genomic Characterization of the Evolutionary Potential of the Sea Urchin Strongylocentrotus droebachiensis Facing Ocean Acidification. Runcie, Daniel E Dorey, Narimane Garfield, David A Stumpp, Meike Dupont, Sam Wray, Gregory A 3672 - 3684 2016-12-01 application/pdf https://escholarship.org/uc/item/02d5f9g3 unknown eScholarship, University of California qt02d5f9g3 https://escholarship.org/uc/item/02d5f9g3 public Genome biology and evolution, vol 8, iss 12 Animals Strongylocentrotus Carbon Dioxide Genomics Seawater Adaptation Physiological Evolution Molecular Oceans and Seas Climate Change RNAseq System genetics gene set variation analysis genetic variation plasticity Developmental Biology Biochemistry and Cell Biology Evolutionary Biology Genetics article 2016 ftcdlib 2020-06-06T07:53:41Z Ocean acidification (OA) is increasing due to anthropogenic CO2 emissions and poses a threat to marine species and communities worldwide. To better project the effects of acidification on organisms' health and persistence, an understanding is needed of the 1) mechanisms underlying developmental and physiological tolerance and 2) potential populations have for rapid evolutionary adaptation. This is especially challenging in nonmodel species where targeted assays of metabolism and stress physiology may not be available or economical for large-scale assessments of genetic constraints. We used mRNA sequencing and a quantitative genetics breeding design to study mechanisms underlying genetic variability and tolerance to decreased seawater pH (-0.4 pH units) in larvae of the sea urchin Strongylocentrotus droebachiensis. We used a gene ontology-based approach to integrate expression profiles into indirect measures of cellular and biochemical traits underlying variation in larval performance (i.e., growth rates). Molecular responses to OA were complex, involving changes to several functions such as growth rates, cell division, metabolism, and immune activities. Surprisingly, the magnitude of pH effects on molecular traits tended to be small relative to variation attributable to segregating functional genetic variation in this species. We discuss how the application of transcriptomics and quantitative genetics approaches across diverse species can enrich our understanding of the biological impacts of climate change. Article in Journal/Newspaper Ocean acidification University of California: eScholarship |
institution |
Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
unknown |
topic |
Animals Strongylocentrotus Carbon Dioxide Genomics Seawater Adaptation Physiological Evolution Molecular Oceans and Seas Climate Change RNAseq System genetics gene set variation analysis genetic variation plasticity Developmental Biology Biochemistry and Cell Biology Evolutionary Biology Genetics |
spellingShingle |
Animals Strongylocentrotus Carbon Dioxide Genomics Seawater Adaptation Physiological Evolution Molecular Oceans and Seas Climate Change RNAseq System genetics gene set variation analysis genetic variation plasticity Developmental Biology Biochemistry and Cell Biology Evolutionary Biology Genetics Runcie, Daniel E Dorey, Narimane Garfield, David A Stumpp, Meike Dupont, Sam Wray, Gregory A Genomic Characterization of the Evolutionary Potential of the Sea Urchin Strongylocentrotus droebachiensis Facing Ocean Acidification. |
topic_facet |
Animals Strongylocentrotus Carbon Dioxide Genomics Seawater Adaptation Physiological Evolution Molecular Oceans and Seas Climate Change RNAseq System genetics gene set variation analysis genetic variation plasticity Developmental Biology Biochemistry and Cell Biology Evolutionary Biology Genetics |
description |
Ocean acidification (OA) is increasing due to anthropogenic CO2 emissions and poses a threat to marine species and communities worldwide. To better project the effects of acidification on organisms' health and persistence, an understanding is needed of the 1) mechanisms underlying developmental and physiological tolerance and 2) potential populations have for rapid evolutionary adaptation. This is especially challenging in nonmodel species where targeted assays of metabolism and stress physiology may not be available or economical for large-scale assessments of genetic constraints. We used mRNA sequencing and a quantitative genetics breeding design to study mechanisms underlying genetic variability and tolerance to decreased seawater pH (-0.4 pH units) in larvae of the sea urchin Strongylocentrotus droebachiensis. We used a gene ontology-based approach to integrate expression profiles into indirect measures of cellular and biochemical traits underlying variation in larval performance (i.e., growth rates). Molecular responses to OA were complex, involving changes to several functions such as growth rates, cell division, metabolism, and immune activities. Surprisingly, the magnitude of pH effects on molecular traits tended to be small relative to variation attributable to segregating functional genetic variation in this species. We discuss how the application of transcriptomics and quantitative genetics approaches across diverse species can enrich our understanding of the biological impacts of climate change. |
format |
Article in Journal/Newspaper |
author |
Runcie, Daniel E Dorey, Narimane Garfield, David A Stumpp, Meike Dupont, Sam Wray, Gregory A |
author_facet |
Runcie, Daniel E Dorey, Narimane Garfield, David A Stumpp, Meike Dupont, Sam Wray, Gregory A |
author_sort |
Runcie, Daniel E |
title |
Genomic Characterization of the Evolutionary Potential of the Sea Urchin Strongylocentrotus droebachiensis Facing Ocean Acidification. |
title_short |
Genomic Characterization of the Evolutionary Potential of the Sea Urchin Strongylocentrotus droebachiensis Facing Ocean Acidification. |
title_full |
Genomic Characterization of the Evolutionary Potential of the Sea Urchin Strongylocentrotus droebachiensis Facing Ocean Acidification. |
title_fullStr |
Genomic Characterization of the Evolutionary Potential of the Sea Urchin Strongylocentrotus droebachiensis Facing Ocean Acidification. |
title_full_unstemmed |
Genomic Characterization of the Evolutionary Potential of the Sea Urchin Strongylocentrotus droebachiensis Facing Ocean Acidification. |
title_sort |
genomic characterization of the evolutionary potential of the sea urchin strongylocentrotus droebachiensis facing ocean acidification. |
publisher |
eScholarship, University of California |
publishDate |
2016 |
url |
https://escholarship.org/uc/item/02d5f9g3 |
op_coverage |
3672 - 3684 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Genome biology and evolution, vol 8, iss 12 |
op_relation |
qt02d5f9g3 https://escholarship.org/uc/item/02d5f9g3 |
op_rights |
public |
_version_ |
1766157626667696128 |