Innovative approach to tissue engineering : development of a biomaterial based on cross-linked salmon collagen and bioactive polyphenols from marine macrophytes

• Main Author: Buscaglia, Manon
• Other Authors: Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Bretagne occidentale - Brest, Gwenaelle Le Blay Laliberte, Fabienne Guérard
• Format: Doctoral or Postdoctoral Thesis
• Language: French
• Published: HAL CCSD 2022
• Subjects: Collagen biomaterial; Enzymatic cross-linked; Polyphenols; Bioactivity; Regenerative medicine; Sustainable process; Biomatériau collagénique; Réticulation enzymatique; Polyphénols; Bioactivité; Médecine régénérative; Procédé écoresponsable; [SDV.BIO]Life Sciences [q-bio]/Biotechnology; Atlantic salmon; Salmo salar
• Online Access: https://theses.hal.science/tel-04213543; https://theses.hal.science/tel-04213543/document; https://theses.hal.science/tel-04213543/file/These-2022-SML-Biotechnologies-BUSCAGLIA_Manon.pdf

The seafood industry generates large quantities of co-products rich in valuable and value-added molecules (e.g. collagen). This major protein of connective tissues is used in bone tissue engineering in order to develop biomaterials mimicking the organic tissue framework. The objective of this thesis is to valorize collagen from Atlantic salmon (Salmo salar) skins by demonstrating its potential as a biomaterial reinforced by enzymatic cross-linking with microbial transglutaminase. This cross-linked collagen was compared to cross-linked porcine collagen and chemical cross-linking with glutaraldehyde (reference values). Thus, the developed cross-linked biomaterial exhibited dose-dependent mechanical properties comparable to the references following the action of the enzyme. In a second step, phenolic compounds, derived from marine macrophytes, with osteogenic and antibacterial properties were sought to be integrated into the biomaterial to stimulate bone regeneration and limit infections in the treated area. Two comparative methodologies were tested for the extraction and the purification of the phenolic compounds, one conventional liquid/liquid method versus one more eco-responsible promising on a Diaion HP-20 resin. Compounds from Beta vulgaris subsp. maritima, Bifurcaria bifurcata and Plantago lanceolata demonstrated the targeted properties, however, both processes resulted in different bioactive compounds obtained. This thesis has allowed to lay the foundations of a new concept in bone tissue engineering, combining a cross-linked salmon collagen hydrogel with marine plant polyphenols presenting antiradical, osteogenic and antimicrobial properties. L’industrie alimentaire des produits de la mer génère une quantité importante de coproduits riches en molécules valorisables (e.g. collagène). Cette protéine majeure des tissus conjonctifs est utilisée en ingénierie tissulaire osseuse pour développer des biomatériaux imitant la trame organique tissulaire. L’objectif de cette thèse est de valoriser le collagène issu ...