Characterization of Anisakidae in marine fish : development of an identification method by high-throughput sequencing and prevalence study
Anisakis, Pseudoterranova, Hysterothylacium and Contracaecum genera, members of the Anisakids family, are nematodes which larvae are recovered from numerous fish and cephalopods species. These larvae may induce digestive and/or allergic pathologies in human being. In France, the consumption of fishe...
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Format: | Doctoral or Postdoctoral Thesis |
Language: | French |
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HAL CCSD
2015
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Online Access: | https://tel.archives-ouvertes.fr/tel-01412878 https://tel.archives-ouvertes.fr/tel-01412878/document https://tel.archives-ouvertes.fr/tel-01412878/file/2015LIL2S043.pdf |
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ftccsdartic:oai:HAL:tel-01412878v1 2023-05-15T15:33:05+02:00 Characterization of Anisakidae in marine fish : development of an identification method by high-throughput sequencing and prevalence study Caractérisation des Anisakidae dans les poissons marins : développement d’une méthode d’identification par séquençage à haut-débit et étude de prévalence Seesao, Yuwalee Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL) Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire Lille (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) Université du Droit et de la Santé - Lille II Eric Viscogliosi 2015-12-08 https://tel.archives-ouvertes.fr/tel-01412878 https://tel.archives-ouvertes.fr/tel-01412878/document https://tel.archives-ouvertes.fr/tel-01412878/file/2015LIL2S043.pdf fr fre HAL CCSD NNT: 2015LIL2S043 tel-01412878 https://tel.archives-ouvertes.fr/tel-01412878 https://tel.archives-ouvertes.fr/tel-01412878/document https://tel.archives-ouvertes.fr/tel-01412878/file/2015LIL2S043.pdf info:eu-repo/semantics/OpenAccess https://tel.archives-ouvertes.fr/tel-01412878 Médecine humaine et pathologie. Université du Droit et de la Santé - Lille II, 2015. Français. ⟨NNT : 2015LIL2S043⟩ Fish Sanger method of sequencing Anisakid family Analyse statistique Biologie moléculaire PCR Séquençage haut débit Sécurité alimentaire Identification Zoonoses Prévalence Intensité Parasitologie Nématodes Poissons Anisakidae [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology info:eu-repo/semantics/doctoralThesis Theses 2015 ftccsdartic 2021-10-24T09:31:14Z Anisakis, Pseudoterranova, Hysterothylacium and Contracaecum genera, members of the Anisakids family, are nematodes which larvae are recovered from numerous fish and cephalopods species. These larvae may induce digestive and/or allergic pathologies in human being. In France, the consumption of fishery products, mostly raw or undercooked is increasing. This PhD work is part of the research program Fish-Parasites funded by the ANR (ANR-10-ALIA-004). It aimed to assess risks related to fishery products consumption and its main goal was to study the distribution of Anisakids in fishery products.The sampling plan was based on a risk-ranking analysis using data on fishery products consumption, fishing areas and prevalence data from previous work on Anisakids. A total of 1 768 fish from 18 species were collected. All the organs were dissected for parasites isolation. Parasites were identified using two approaches : i) single analysis by Sanger sequencing for organs containing less than 11 nematodes ii) pooled analysis by high throughput sequencing (HTS) for the remaining. The development of numerous tools (database creation with reference sequences, primers design, set up of the sequencing template preparation and development of an automatic analytical pipeline) was necessary for the HTS method set up. From the sequencing results, acquisition and structuring of the prevalence data has been carried out on parasites potentially pathogenic for human being and recovered from commonly consumed fishery products.On 1 768 sampled fish, two species were not parasitized at all: plaice (Pleuronectes platessa) and aquacultured Atlantic salmon (Salmo salar). 43.30 % of the fish were not infected by Anisakids. Concerning infected fish, 28.62% were contaminated in the visceral organs; 22.96% in both visceral organs and fillets and, finally, 5.49% of the fish were infected by Anisakids only in fillets.The five fish species with an elevated prevalence in their fillets were by decreasing values: blue ling (100 %), megrim (70 %), saithe ... Doctoral or Postdoctoral Thesis Atlantic salmon Salmo salar Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
French |
topic |
Fish Sanger method of sequencing Anisakid family Analyse statistique Biologie moléculaire PCR Séquençage haut débit Sécurité alimentaire Identification Zoonoses Prévalence Intensité Parasitologie Nématodes Poissons Anisakidae [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
spellingShingle |
Fish Sanger method of sequencing Anisakid family Analyse statistique Biologie moléculaire PCR Séquençage haut débit Sécurité alimentaire Identification Zoonoses Prévalence Intensité Parasitologie Nématodes Poissons Anisakidae [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology Seesao, Yuwalee Characterization of Anisakidae in marine fish : development of an identification method by high-throughput sequencing and prevalence study |
topic_facet |
Fish Sanger method of sequencing Anisakid family Analyse statistique Biologie moléculaire PCR Séquençage haut débit Sécurité alimentaire Identification Zoonoses Prévalence Intensité Parasitologie Nématodes Poissons Anisakidae [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
description |
Anisakis, Pseudoterranova, Hysterothylacium and Contracaecum genera, members of the Anisakids family, are nematodes which larvae are recovered from numerous fish and cephalopods species. These larvae may induce digestive and/or allergic pathologies in human being. In France, the consumption of fishery products, mostly raw or undercooked is increasing. This PhD work is part of the research program Fish-Parasites funded by the ANR (ANR-10-ALIA-004). It aimed to assess risks related to fishery products consumption and its main goal was to study the distribution of Anisakids in fishery products.The sampling plan was based on a risk-ranking analysis using data on fishery products consumption, fishing areas and prevalence data from previous work on Anisakids. A total of 1 768 fish from 18 species were collected. All the organs were dissected for parasites isolation. Parasites were identified using two approaches : i) single analysis by Sanger sequencing for organs containing less than 11 nematodes ii) pooled analysis by high throughput sequencing (HTS) for the remaining. The development of numerous tools (database creation with reference sequences, primers design, set up of the sequencing template preparation and development of an automatic analytical pipeline) was necessary for the HTS method set up. From the sequencing results, acquisition and structuring of the prevalence data has been carried out on parasites potentially pathogenic for human being and recovered from commonly consumed fishery products.On 1 768 sampled fish, two species were not parasitized at all: plaice (Pleuronectes platessa) and aquacultured Atlantic salmon (Salmo salar). 43.30 % of the fish were not infected by Anisakids. Concerning infected fish, 28.62% were contaminated in the visceral organs; 22.96% in both visceral organs and fillets and, finally, 5.49% of the fish were infected by Anisakids only in fillets.The five fish species with an elevated prevalence in their fillets were by decreasing values: blue ling (100 %), megrim (70 %), saithe ... |
author2 |
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL) Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire Lille (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) Université du Droit et de la Santé - Lille II Eric Viscogliosi |
format |
Doctoral or Postdoctoral Thesis |
author |
Seesao, Yuwalee |
author_facet |
Seesao, Yuwalee |
author_sort |
Seesao, Yuwalee |
title |
Characterization of Anisakidae in marine fish : development of an identification method by high-throughput sequencing and prevalence study |
title_short |
Characterization of Anisakidae in marine fish : development of an identification method by high-throughput sequencing and prevalence study |
title_full |
Characterization of Anisakidae in marine fish : development of an identification method by high-throughput sequencing and prevalence study |
title_fullStr |
Characterization of Anisakidae in marine fish : development of an identification method by high-throughput sequencing and prevalence study |
title_full_unstemmed |
Characterization of Anisakidae in marine fish : development of an identification method by high-throughput sequencing and prevalence study |
title_sort |
characterization of anisakidae in marine fish : development of an identification method by high-throughput sequencing and prevalence study |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://tel.archives-ouvertes.fr/tel-01412878 https://tel.archives-ouvertes.fr/tel-01412878/document https://tel.archives-ouvertes.fr/tel-01412878/file/2015LIL2S043.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
https://tel.archives-ouvertes.fr/tel-01412878 Médecine humaine et pathologie. Université du Droit et de la Santé - Lille II, 2015. Français. ⟨NNT : 2015LIL2S043⟩ |
op_relation |
NNT: 2015LIL2S043 tel-01412878 https://tel.archives-ouvertes.fr/tel-01412878 https://tel.archives-ouvertes.fr/tel-01412878/document https://tel.archives-ouvertes.fr/tel-01412878/file/2015LIL2S043.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1766363558975635456 |