Lipase-catalyzed purification and functionalization of Omega-3 polyunsaturated fatty acids and production of structured lipids
Lipases are enzymes with applications extended to a wide variety of industries. The variety of lipases applications led to increased research to characterize them and better understand their kinetics and reaction mechanisms and to establish methods for lipase production in homologous and heterologou...
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HAL CCSD
2012
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ftccsdartic:oai:HAL:tel-01077665v1 2023-05-15T13:56:06+02:00 Lipase-catalyzed purification and functionalization of Omega-3 polyunsaturated fatty acids and production of structured lipids Purification et fonctionnalisation d’acides gras polyinsaturés Oméga-3 par des lipases et production de lipides structurés Casas Godoy, Leticia Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP) Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS) INSA de Toulouse Universidade técnica (Lisbonne) Alain Marty Suzana Ferreira Dias 2012-12-14 https://tel.archives-ouvertes.fr/tel-01077665 https://tel.archives-ouvertes.fr/tel-01077665/document https://tel.archives-ouvertes.fr/tel-01077665/file/2012Casas_-_Godoy.pdf en eng HAL CCSD NNT: 2012ISAT0057 tel-01077665 https://tel.archives-ouvertes.fr/tel-01077665 https://tel.archives-ouvertes.fr/tel-01077665/document https://tel.archives-ouvertes.fr/tel-01077665/file/2012Casas_-_Godoy.pdf info:eu-repo/semantics/OpenAccess https://tel.archives-ouvertes.fr/tel-01077665 Food and Nutrition. INSA de Toulouse; Universidade técnica (Lisbonne), 2012. English. ⟨NNT : 2012ISAT0057⟩ Structured lipids Selectivity Mutagenesis Fish oils Immobilization Lipase Huile de poisson Purification DHA Mutagenèse Sélectivité Lipides structurés Immobilisation Omega-3 Yarrowia lipolytica [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition info:eu-repo/semantics/doctoralThesis Theses 2012 ftccsdartic 2021-10-24T12:53:19Z Lipases are enzymes with applications extended to a wide variety of industries. The variety of lipases applications led to increased research to characterize them and better understand their kinetics and reaction mechanisms and to establish methods for lipase production in homologous and heterologous expression systems. Lately enzymatic engineering allowed the improvement of lipase characteristics. This thesis project studies the use of lipases for two main objectives: lipase-catalyzed purification and functionalization of Omega-3 polyunsaturated fatty acids (PUFAs), especially cis-4, 7, 10, 13, 16, 19-docosahexaenoic acid (DHA) and production of structured lipids (SL). DHA was used for the synthesis of a pharmaceutical molecule, the nicotinyl DHA ester. The co-substrate of the reaction was nicotinol, an alcohol from the group B pro-vitamin, which after absorption is rapidly converted into nicotinic acid (Vitamin B3). The enzymatic trans-esterification of DHA ethyl esters with nicotinol was optimised to synthesise an ester presenting the cumulative properties of the two reactants. After enzyme (immobilized lipase from Candida antarctica; Novozym 435) and reaction medium (solvent-free system) selection, the process was optimised. A conversion to nicotinyl-DHA superior to 97 % was obtained in 4 hours using 45 g.L-1 of enzyme. With a productivity of 4.2 g of product .h-1.g of enzyme-1.This project requires DHA of high purity. Enzymatic purification was chosen for the production of DHA concentrates. Lipases can discriminate between fatty acids in function of their chain length and saturation degree. Lipases react more efficiently with the bulk of saturated and mono-unsaturated fatty acids than with the PUFAs. The objective was the discovery of more specific enzymes for DHA purification. The lipase Lip2 from Yarrowia lipolytica (YLL2) appears as a good candidate since it is homologous to one of the most efficient lipase, the lipase from Thermomyces lanuginosus. YLL2 enables a high discrimination to be obtained, ... Doctoral or Postdoctoral Thesis Antarc* Antarctica Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Structured lipids Selectivity Mutagenesis Fish oils Immobilization Lipase Huile de poisson Purification DHA Mutagenèse Sélectivité Lipides structurés Immobilisation Omega-3 Yarrowia lipolytica [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
spellingShingle |
Structured lipids Selectivity Mutagenesis Fish oils Immobilization Lipase Huile de poisson Purification DHA Mutagenèse Sélectivité Lipides structurés Immobilisation Omega-3 Yarrowia lipolytica [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Casas Godoy, Leticia Lipase-catalyzed purification and functionalization of Omega-3 polyunsaturated fatty acids and production of structured lipids |
topic_facet |
Structured lipids Selectivity Mutagenesis Fish oils Immobilization Lipase Huile de poisson Purification DHA Mutagenèse Sélectivité Lipides structurés Immobilisation Omega-3 Yarrowia lipolytica [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
description |
Lipases are enzymes with applications extended to a wide variety of industries. The variety of lipases applications led to increased research to characterize them and better understand their kinetics and reaction mechanisms and to establish methods for lipase production in homologous and heterologous expression systems. Lately enzymatic engineering allowed the improvement of lipase characteristics. This thesis project studies the use of lipases for two main objectives: lipase-catalyzed purification and functionalization of Omega-3 polyunsaturated fatty acids (PUFAs), especially cis-4, 7, 10, 13, 16, 19-docosahexaenoic acid (DHA) and production of structured lipids (SL). DHA was used for the synthesis of a pharmaceutical molecule, the nicotinyl DHA ester. The co-substrate of the reaction was nicotinol, an alcohol from the group B pro-vitamin, which after absorption is rapidly converted into nicotinic acid (Vitamin B3). The enzymatic trans-esterification of DHA ethyl esters with nicotinol was optimised to synthesise an ester presenting the cumulative properties of the two reactants. After enzyme (immobilized lipase from Candida antarctica; Novozym 435) and reaction medium (solvent-free system) selection, the process was optimised. A conversion to nicotinyl-DHA superior to 97 % was obtained in 4 hours using 45 g.L-1 of enzyme. With a productivity of 4.2 g of product .h-1.g of enzyme-1.This project requires DHA of high purity. Enzymatic purification was chosen for the production of DHA concentrates. Lipases can discriminate between fatty acids in function of their chain length and saturation degree. Lipases react more efficiently with the bulk of saturated and mono-unsaturated fatty acids than with the PUFAs. The objective was the discovery of more specific enzymes for DHA purification. The lipase Lip2 from Yarrowia lipolytica (YLL2) appears as a good candidate since it is homologous to one of the most efficient lipase, the lipase from Thermomyces lanuginosus. YLL2 enables a high discrimination to be obtained, ... |
author2 |
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP) Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS) INSA de Toulouse Universidade técnica (Lisbonne) Alain Marty Suzana Ferreira Dias |
format |
Doctoral or Postdoctoral Thesis |
author |
Casas Godoy, Leticia |
author_facet |
Casas Godoy, Leticia |
author_sort |
Casas Godoy, Leticia |
title |
Lipase-catalyzed purification and functionalization of Omega-3 polyunsaturated fatty acids and production of structured lipids |
title_short |
Lipase-catalyzed purification and functionalization of Omega-3 polyunsaturated fatty acids and production of structured lipids |
title_full |
Lipase-catalyzed purification and functionalization of Omega-3 polyunsaturated fatty acids and production of structured lipids |
title_fullStr |
Lipase-catalyzed purification and functionalization of Omega-3 polyunsaturated fatty acids and production of structured lipids |
title_full_unstemmed |
Lipase-catalyzed purification and functionalization of Omega-3 polyunsaturated fatty acids and production of structured lipids |
title_sort |
lipase-catalyzed purification and functionalization of omega-3 polyunsaturated fatty acids and production of structured lipids |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://tel.archives-ouvertes.fr/tel-01077665 https://tel.archives-ouvertes.fr/tel-01077665/document https://tel.archives-ouvertes.fr/tel-01077665/file/2012Casas_-_Godoy.pdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
https://tel.archives-ouvertes.fr/tel-01077665 Food and Nutrition. INSA de Toulouse; Universidade técnica (Lisbonne), 2012. English. ⟨NNT : 2012ISAT0057⟩ |
op_relation |
NNT: 2012ISAT0057 tel-01077665 https://tel.archives-ouvertes.fr/tel-01077665 https://tel.archives-ouvertes.fr/tel-01077665/document https://tel.archives-ouvertes.fr/tel-01077665/file/2012Casas_-_Godoy.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1766263356969189376 |