Evaluation of the deleterious effects of heavy metals and pesticides on early life stages and gametes of the Pacific Oyster, Crassostrea gigas : application to the pollution context of the Arcachon Bay

• Main Author: Mai, Huong
• Other Authors: Environnements et Paléoenvironnements OCéaniques (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Université Sciences et Technologies - Bordeaux I, Université de Technologie de Hanoï. Vietnam, Jérôme Cachot, Bénédicte Morin
• Format: Doctoral or Postdoctoral Thesis
• Language: English
• Published: HAL CCSD 2013
• Subjects: Pesticides; Metals; Pacific oyster; Embryo-larvae toxicity assay; Fertilization assay; Comet assay; Gene expression; Expression de gènes; Test de fertilité; Génotoxicité; Test embryo-larvaire; Huitre creuse; Métaux; [SDV.SA]Life Sciences [q-bio]/Agricultural sciences; [SDV.BA]Life Sciences [q-bio]/Animal biology; Pacific; Crassostrea gigas
• Online Access: https://tel.archives-ouvertes.fr/tel-01053814; https://tel.archives-ouvertes.fr/tel-01053814/document; https://tel.archives-ouvertes.fr/tel-01053814/file/MAI_HUONG_2013.pdf

The coastal areas are subject to multiple anthropogenic pressures including chemical pollution that can pose a real risk to the sustainability of aquatic species. The Arcachon Bay, macrotidal lagoon located on the French Atlantic coast, is the important ecosystem for oyster farming. But for several years, the oyster farms face lower recruitment and high mortality of oyster spat. Chemical contamination of the environment as a factor that may contribute to the observed effects on oysters has so far not been investigated.The present thesis aimed at evaluating through different approaches, of the potential toxicity of heavy metals and pesticides representative of the Arcachon Bay contamination on the early life stages of the Pacific oyster, Crassostrea gigas. Embryotoxicity, genotoxicity and expression levels of eleven targeted genes were studied. Firstly, different pesticides (S-metolachlor, irgarol, and diuron) and metals (copper and cadmium) were separately tested to determine their spectrum of effects. It were shown that exposure of gametes and embryos of oyster to environmental concentrations of pesticides and copper increased developmental abnormalities and DNA damage, and reduced fertilization success and affected offpring quality. Cadmium, meanwhile, showed no embryotoxic and genotoxic effects at the concentrations found in the Arcachon Bay. Metabolites of metolachlor, metolachlor ESA and metolachlor OA, are found in the Arcachon Bay at higher concentrations than their parent compound. The results showed that these metabolites were less embryotoxic and genotoxic on oyster embryos and spermatozoa than metolachor. Significant changes in expression of genes involved in antioxidant defense were observed for oyster larvae exposed to metolachlor and metolachlor ESA. Toxicity of mixtures of pesticides representative of the Arcachon Bay contamination with and without copper was then evaluated. Exposures of oyster embryos to these mixtures lead to development defects, DNA damage and changes in the expression of genes ...