Elements implicated in the energypathway of the AMP-activated protein kinase of the Pacific oyster Crassostrea gigas
The objective of this thesis was to characterize elements implicated in the energypathway of the AMP-activated protein kinase of the Pacific oyster Crassostrea gigas. Thecharacterization of the elements was performed in the scope of understanding their involvementin energy management, particularly i...
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Format: | Doctoral or Postdoctoral Thesis |
Language: | French |
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HAL CCSD
2012
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Online Access: | https://tel.archives-ouvertes.fr/tel-00863226 https://tel.archives-ouvertes.fr/tel-00863226/document https://tel.archives-ouvertes.fr/tel-00863226/file/These-2012-EDSM-Biologie_marine-GUEVELOU_Eric.pdf |
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ftccsdartic:oai:HAL:tel-00863226v1 2023-05-15T15:57:41+02:00 Elements implicated in the energypathway of the AMP-activated protein kinase of the Pacific oyster Crassostrea gigas Étude fonctionnelle de l'AMP-activated protein kinase chez l'huître creuse Crassostrea gigas Guévélou, Éric Laboratoire de Physiologie des Invertébrés (LPI) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Université de Bretagne occidentale - Brest Arnaud Huvet 2012-12-19 https://tel.archives-ouvertes.fr/tel-00863226 https://tel.archives-ouvertes.fr/tel-00863226/document https://tel.archives-ouvertes.fr/tel-00863226/file/These-2012-EDSM-Biologie_marine-GUEVELOU_Eric.pdf fr fre HAL CCSD NNT: 2012BRES0031 tel-00863226 https://tel.archives-ouvertes.fr/tel-00863226 https://tel.archives-ouvertes.fr/tel-00863226/document https://tel.archives-ouvertes.fr/tel-00863226/file/These-2012-EDSM-Biologie_marine-GUEVELOU_Eric.pdf info:eu-repo/semantics/OpenAccess https://tel.archives-ouvertes.fr/tel-00863226 Biologie animale. Université de Bretagne occidentale - Brest, 2012. Français. ⟨NNT : 2012BRES0031⟩ Hypoxia Reproduction Energetic pathways Voie énergétique Crassostrea gigas AMP-activated protein kinase Hypoxie [SDV.BA]Life Sciences [q-bio]/Animal biology info:eu-repo/semantics/doctoralThesis Theses 2012 ftccsdartic 2021-04-03T23:44:01Z The objective of this thesis was to characterize elements implicated in the energypathway of the AMP-activated protein kinase of the Pacific oyster Crassostrea gigas. Thecharacterization of the elements was performed in the scope of understanding their involvementin energy management, particularly in response to physiological conditions requiring energy, asreproduction or environmental stress, such as hypoxia or fasting.At genomic level, the three subunits of AMPK trimer and several elements involved inAMPK signaling pathway and in carbohydrate and lipid metabolism, supposedly under AMPKcontrol, were described. Additionally, at proteomic level, several heterologous antibodiestargeting AMPKα subunit isoforms and threonine 172 phosphorylation site of AMPKα subunit,indirect witness of AMPK activity, were assayed. Two truncated α subunits in the kinase domainwere characterized essentially in muscles, suggesting their involvement in muscle function.During a hypoxic stress, a significant increase of truncated α subunits protein amount wasobserved in smooth muscle. These results suggest that, for a period of at least 6 h, thesetruncated subunits are necessary for the maintenance of aerobic metabolism in smooth muscle ofC. gigas, allowing it to fulfill its static closing valves. We suggested that in vivo accumulation oftruncated AMPKα could serve as modulator or as transdominant negative regulator of the fulllengthAMPKα activity. In the gonad, AMPK appeared to be activated through the process ofgametogenesis, in order to support the catabolic processes of gametes creation. During theanabolic phase, when oocyte reserves were created, a signal disruption was observed. Finally,during controlled experiment, a physiological approach by food deprivation and apharmacological approach using AICAR injections were performed to modulate AMPK signal.This analysis showed that neither fasting nor AICAR induced an increase of AMPKphosphorylation, as expected. Although, several changes related to AICAR injection wereobserved in oysters ... Doctoral or Postdoctoral Thesis Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
French |
topic |
Hypoxia Reproduction Energetic pathways Voie énergétique Crassostrea gigas AMP-activated protein kinase Hypoxie [SDV.BA]Life Sciences [q-bio]/Animal biology |
spellingShingle |
Hypoxia Reproduction Energetic pathways Voie énergétique Crassostrea gigas AMP-activated protein kinase Hypoxie [SDV.BA]Life Sciences [q-bio]/Animal biology Guévélou, Éric Elements implicated in the energypathway of the AMP-activated protein kinase of the Pacific oyster Crassostrea gigas |
topic_facet |
Hypoxia Reproduction Energetic pathways Voie énergétique Crassostrea gigas AMP-activated protein kinase Hypoxie [SDV.BA]Life Sciences [q-bio]/Animal biology |
description |
The objective of this thesis was to characterize elements implicated in the energypathway of the AMP-activated protein kinase of the Pacific oyster Crassostrea gigas. Thecharacterization of the elements was performed in the scope of understanding their involvementin energy management, particularly in response to physiological conditions requiring energy, asreproduction or environmental stress, such as hypoxia or fasting.At genomic level, the three subunits of AMPK trimer and several elements involved inAMPK signaling pathway and in carbohydrate and lipid metabolism, supposedly under AMPKcontrol, were described. Additionally, at proteomic level, several heterologous antibodiestargeting AMPKα subunit isoforms and threonine 172 phosphorylation site of AMPKα subunit,indirect witness of AMPK activity, were assayed. Two truncated α subunits in the kinase domainwere characterized essentially in muscles, suggesting their involvement in muscle function.During a hypoxic stress, a significant increase of truncated α subunits protein amount wasobserved in smooth muscle. These results suggest that, for a period of at least 6 h, thesetruncated subunits are necessary for the maintenance of aerobic metabolism in smooth muscle ofC. gigas, allowing it to fulfill its static closing valves. We suggested that in vivo accumulation oftruncated AMPKα could serve as modulator or as transdominant negative regulator of the fulllengthAMPKα activity. In the gonad, AMPK appeared to be activated through the process ofgametogenesis, in order to support the catabolic processes of gametes creation. During theanabolic phase, when oocyte reserves were created, a signal disruption was observed. Finally,during controlled experiment, a physiological approach by food deprivation and apharmacological approach using AICAR injections were performed to modulate AMPK signal.This analysis showed that neither fasting nor AICAR induced an increase of AMPKphosphorylation, as expected. Although, several changes related to AICAR injection wereobserved in oysters ... |
author2 |
Laboratoire de Physiologie des Invertébrés (LPI) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Université de Bretagne occidentale - Brest Arnaud Huvet |
format |
Doctoral or Postdoctoral Thesis |
author |
Guévélou, Éric |
author_facet |
Guévélou, Éric |
author_sort |
Guévélou, Éric |
title |
Elements implicated in the energypathway of the AMP-activated protein kinase of the Pacific oyster Crassostrea gigas |
title_short |
Elements implicated in the energypathway of the AMP-activated protein kinase of the Pacific oyster Crassostrea gigas |
title_full |
Elements implicated in the energypathway of the AMP-activated protein kinase of the Pacific oyster Crassostrea gigas |
title_fullStr |
Elements implicated in the energypathway of the AMP-activated protein kinase of the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Elements implicated in the energypathway of the AMP-activated protein kinase of the Pacific oyster Crassostrea gigas |
title_sort |
elements implicated in the energypathway of the amp-activated protein kinase of the pacific oyster crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://tel.archives-ouvertes.fr/tel-00863226 https://tel.archives-ouvertes.fr/tel-00863226/document https://tel.archives-ouvertes.fr/tel-00863226/file/These-2012-EDSM-Biologie_marine-GUEVELOU_Eric.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
https://tel.archives-ouvertes.fr/tel-00863226 Biologie animale. Université de Bretagne occidentale - Brest, 2012. Français. ⟨NNT : 2012BRES0031⟩ |
op_relation |
NNT: 2012BRES0031 tel-00863226 https://tel.archives-ouvertes.fr/tel-00863226 https://tel.archives-ouvertes.fr/tel-00863226/document https://tel.archives-ouvertes.fr/tel-00863226/file/These-2012-EDSM-Biologie_marine-GUEVELOU_Eric.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1766393357866631168 |