Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon
International audience Gamma carbonic anhydrases (cCA) are widespread in Prokaryotes. In Eukaryotes, homologous genes were found only in plant genomes. In Arabidopsis and maize, the corresponding gene products are subunits of mitochondrial Complex I. At present, only cCA homotrimers of Methanosarcin...
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ftccsdartic:oai:HAL:pasteur-03525339v1 2023-11-12T04:15:47+01:00 Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon Martin, Victoria Villarreal, Fernando Miras, Isabelle Navaza, Alda Haouz, Ahmed González-Lebrero, Rodolfo, M. Kaufman, Sergio, B. Zabaleta, Eduardo Instituto de Investigaciones Biológicas Mar del Plata (IIB) Consejo Nacional de Investigaciones Científicas y Técnicas Buenos Aires (CONICET)-Facultad de Ciencias Exactas y Naturales Mar del Plata Universidad Nacional de Mar del Plata Mar del Plata (UNMdP)-Universidad Nacional de Mar del Plata Mar del Plata (UNMdP) Cristallogenèse et Diffraction des Rayons X (Plate-forme/PF6) Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS) Laboratoire Acides Nucléiques & Biophotonique (AnBiophi) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Universidad de Buenos Aires Buenos Aires (UBA) This work was supported by Grants from the (ANPCyT, PICT 31669 and 00673), the Ministry of Science and Technology, Argentine-ECOS Sud France (MinCyT-ECOS A06B03) and the University of Mar del Plata, Argentine (OCS 341/08). V.M. and F.V. are doctoral fellows, S.K., R.G.L. and E.Z. are members of CONICET. 2009-10-04 https://pasteur.hal.science/pasteur-03525339 https://doi.org/10.1016/j.febslet.2009.09.055 en eng HAL CCSD info:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2009.09.055 info:eu-repo/semantics/altIdentifier/pmid/19808034 pasteur-03525339 https://pasteur.hal.science/pasteur-03525339 doi:10.1016/j.febslet.2009.09.055 PUBMED: 19808034 ISSN: 0014-5793 EISSN: 0014-5793 FEBS Letters https://pasteur.hal.science/pasteur-03525339 FEBS Letters, 2009, 583 (21), pp.3425 - 3430. ⟨10.1016/j.febslet.2009.09.055⟩ Gamma carbonic anhydrase Carbon dioxide Carbonate Mitochondria Arabidopsis thaliana MESH: Amino Acid Sequence MESH: Arabidopsis MESH: Protein Structure Quaternary MESH: Protein Subunits MESH: Recombinant Proteins MESH: Solubility MESH: Water MESH: Bicarbonates MESH: Carbon MESH: Carbon Radioisotopes MESH: Carbonic Acid MESH: Carbonic Anhydrase II MESH: Molecular Sequence Data MESH: Protein Binding MESH: Protein Multimerization [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2009 ftccsdartic https://doi.org/10.1016/j.febslet.2009.09.055 2023-10-28T22:46:32Z International audience Gamma carbonic anhydrases (cCA) are widespread in Prokaryotes. In Eukaryotes, homologous genes were found only in plant genomes. In Arabidopsis and maize, the corresponding gene products are subunits of mitochondrial Complex I. At present, only cCA homotrimers of Methanosarcina thermophila (CAM) show reversible carbon dioxide (CO 2) hydration activity. In the present work, it is shown that recombinant plant cCA2 could form homotrimers and bind H 14 CO À 3. However, they are unable to catalyse the reversible hydration of CO 2. These results suggest that plant cCAs do not act as carbonic anhydrases but with a related activity possibly contributing to recycle CO 2 in the context of photorespiration. Article in Journal/Newspaper Carbonic acid Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) FEBS Letters 583 21 3425 3430 |
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Open Polar |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
Gamma carbonic anhydrase Carbon dioxide Carbonate Mitochondria Arabidopsis thaliana MESH: Amino Acid Sequence MESH: Arabidopsis MESH: Protein Structure Quaternary MESH: Protein Subunits MESH: Recombinant Proteins MESH: Solubility MESH: Water MESH: Bicarbonates MESH: Carbon MESH: Carbon Radioisotopes MESH: Carbonic Acid MESH: Carbonic Anhydrase II MESH: Molecular Sequence Data MESH: Protein Binding MESH: Protein Multimerization [SDV]Life Sciences [q-bio] |
spellingShingle |
Gamma carbonic anhydrase Carbon dioxide Carbonate Mitochondria Arabidopsis thaliana MESH: Amino Acid Sequence MESH: Arabidopsis MESH: Protein Structure Quaternary MESH: Protein Subunits MESH: Recombinant Proteins MESH: Solubility MESH: Water MESH: Bicarbonates MESH: Carbon MESH: Carbon Radioisotopes MESH: Carbonic Acid MESH: Carbonic Anhydrase II MESH: Molecular Sequence Data MESH: Protein Binding MESH: Protein Multimerization [SDV]Life Sciences [q-bio] Martin, Victoria Villarreal, Fernando Miras, Isabelle Navaza, Alda Haouz, Ahmed González-Lebrero, Rodolfo, M. Kaufman, Sergio, B. Zabaleta, Eduardo Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon |
topic_facet |
Gamma carbonic anhydrase Carbon dioxide Carbonate Mitochondria Arabidopsis thaliana MESH: Amino Acid Sequence MESH: Arabidopsis MESH: Protein Structure Quaternary MESH: Protein Subunits MESH: Recombinant Proteins MESH: Solubility MESH: Water MESH: Bicarbonates MESH: Carbon MESH: Carbon Radioisotopes MESH: Carbonic Acid MESH: Carbonic Anhydrase II MESH: Molecular Sequence Data MESH: Protein Binding MESH: Protein Multimerization [SDV]Life Sciences [q-bio] |
description |
International audience Gamma carbonic anhydrases (cCA) are widespread in Prokaryotes. In Eukaryotes, homologous genes were found only in plant genomes. In Arabidopsis and maize, the corresponding gene products are subunits of mitochondrial Complex I. At present, only cCA homotrimers of Methanosarcina thermophila (CAM) show reversible carbon dioxide (CO 2) hydration activity. In the present work, it is shown that recombinant plant cCA2 could form homotrimers and bind H 14 CO À 3. However, they are unable to catalyse the reversible hydration of CO 2. These results suggest that plant cCAs do not act as carbonic anhydrases but with a related activity possibly contributing to recycle CO 2 in the context of photorespiration. |
author2 |
Instituto de Investigaciones Biológicas Mar del Plata (IIB) Consejo Nacional de Investigaciones Científicas y Técnicas Buenos Aires (CONICET)-Facultad de Ciencias Exactas y Naturales Mar del Plata Universidad Nacional de Mar del Plata Mar del Plata (UNMdP)-Universidad Nacional de Mar del Plata Mar del Plata (UNMdP) Cristallogenèse et Diffraction des Rayons X (Plate-forme/PF6) Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS) Laboratoire Acides Nucléiques & Biophotonique (AnBiophi) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Universidad de Buenos Aires Buenos Aires (UBA) This work was supported by Grants from the (ANPCyT, PICT 31669 and 00673), the Ministry of Science and Technology, Argentine-ECOS Sud France (MinCyT-ECOS A06B03) and the University of Mar del Plata, Argentine (OCS 341/08). V.M. and F.V. are doctoral fellows, S.K., R.G.L. and E.Z. are members of CONICET. |
format |
Article in Journal/Newspaper |
author |
Martin, Victoria Villarreal, Fernando Miras, Isabelle Navaza, Alda Haouz, Ahmed González-Lebrero, Rodolfo, M. Kaufman, Sergio, B. Zabaleta, Eduardo |
author_facet |
Martin, Victoria Villarreal, Fernando Miras, Isabelle Navaza, Alda Haouz, Ahmed González-Lebrero, Rodolfo, M. Kaufman, Sergio, B. Zabaleta, Eduardo |
author_sort |
Martin, Victoria |
title |
Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon |
title_short |
Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon |
title_full |
Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon |
title_fullStr |
Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon |
title_full_unstemmed |
Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon |
title_sort |
recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://pasteur.hal.science/pasteur-03525339 https://doi.org/10.1016/j.febslet.2009.09.055 |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_source |
ISSN: 0014-5793 EISSN: 0014-5793 FEBS Letters https://pasteur.hal.science/pasteur-03525339 FEBS Letters, 2009, 583 (21), pp.3425 - 3430. ⟨10.1016/j.febslet.2009.09.055⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2009.09.055 info:eu-repo/semantics/altIdentifier/pmid/19808034 pasteur-03525339 https://pasteur.hal.science/pasteur-03525339 doi:10.1016/j.febslet.2009.09.055 PUBMED: 19808034 |
op_doi |
https://doi.org/10.1016/j.febslet.2009.09.055 |
container_title |
FEBS Letters |
container_volume |
583 |
container_issue |
21 |
container_start_page |
3425 |
op_container_end_page |
3430 |
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1782333059848208384 |