Amino acid structure and characterization of a heterodimeric disintegrin from Vipera lebetina venom
Referred to by :Ammar Gasmi, Najet Srairi, Sami Guermazi, Hafedh Dekhil, Habib Karoui, Mohamed ElAyebErratum to “Amino acid structure and characterization of a heterodimeric disintegrin from Vipera lebetina venom” [Biochim. Biophys. Acta 1547 (2001) 51–56]Biochimica et Biophysica Acta (BBA) - Protei...
Published in: | Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology |
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Online Access: | https://hal-riip.archives-ouvertes.fr/pasteur-02026135 https://doi.org/10.1016/S0167-4838(01)00168-6 |
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ftccsdartic:oai:HAL:pasteur-02026135v1 2023-05-15T18:13:10+02:00 Amino acid structure and characterization of a heterodimeric disintegrin from Vipera lebetina venom Gasmi, Ammar Srairi, Najet Guermazi, Sami Dkhil, Hafedh Karoui, Habib El Ayeb, Mohamed Laboratoire des Venins et Toxines, Institut Pasteur de Tunis Institut Pasteur de Tunis Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) Laboratoire d'Hématologie, Institut Pasteur de Tunis The authors express their gratitude to P.Y. Haumont from Perkin-Elmer Biosystems (France) for the mass determination of lebein by mass spectrometry and to Dr. Z. Ben Lasfar for providing snake venom. 2001-05 https://hal-riip.archives-ouvertes.fr/pasteur-02026135 https://doi.org/10.1016/S0167-4838(01)00168-6 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/S0167-4838(01)00168-6 info:eu-repo/semantics/altIdentifier/pmid/11343790 pasteur-02026135 https://hal-riip.archives-ouvertes.fr/pasteur-02026135 doi:10.1016/S0167-4838(01)00168-6 PUBMED: 11343790 ISSN: 0167-4838 Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology https://hal-riip.archives-ouvertes.fr/pasteur-02026135 Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Elsevier, 2001, 1547 (1), pp.51-56. ⟨10.1016/S0167-4838(01)00168-6⟩ Lebein Amino acid sequence Heterodimeric disintegrin MESH: Amino Acid Sequence MESH: Chromatography Gel MESH: Viper Venoms/isolation & purification MESH: Disintegrins/chemistry* MESH: Disintegrins/isolation & purification MESH: Mass Spectrometry MESH: Molecular Sequence Data MESH: Platelet Aggregation Inhibitors/chemistry* MESH: Platelet Aggregation Inhibitors/isolation & purification MESH: Sequence Alignment MESH: Viper Venoms/chemistry* [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2001 ftccsdartic https://doi.org/10.1016/S0167-4838(01)00168-6 2020-12-24T21:44:58Z Referred to by :Ammar Gasmi, Najet Srairi, Sami Guermazi, Hafedh Dekhil, Habib Karoui, Mohamed ElAyebErratum to “Amino acid structure and characterization of a heterodimeric disintegrin from Vipera lebetina venom” [Biochim. Biophys. Acta 1547 (2001) 51–56]Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Volume 1764, Issue 9, September 2006, Pages 1525 International audience A heterodimeric disintegrin designed as lebein was isolated from crude Vipera lebetina venom using gel filtration, anion and cation exchange chromatographies on FPLC. The amino acid sequence of each subunit determined by Edman degradation contains 64 residues with ten half-cystines and an RGD site at the C-terminal part of the molecule. The molecular mass of native lebein determined by mass spectrometry was found to be 14 083.4 Da and those of α and β subunits were 6992.05 and 7117.62, respectively. These value are in good agreement with those calculated from the sequences. This protein strongly inhibits ADP induced platelet aggregation on human platelet rich plasma with IC50=160 nM. Sequences of this protein subunits displayed significant sequence similarities with many other monomeric and dimeric disintegrins reported from snake venoms. We identified an amino acid residue (N) in the hairpin loop of both subunits (CRARGDDMNDYC) which is different from all other reported motifs of disintegrins and this subtle difference may contribute to the distinct affinities and selectivities of this class of proteins. Article in Journal/Newspaper sami Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 1547 1 51 56 |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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English |
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Lebein Amino acid sequence Heterodimeric disintegrin MESH: Amino Acid Sequence MESH: Chromatography Gel MESH: Viper Venoms/isolation & purification MESH: Disintegrins/chemistry* MESH: Disintegrins/isolation & purification MESH: Mass Spectrometry MESH: Molecular Sequence Data MESH: Platelet Aggregation Inhibitors/chemistry* MESH: Platelet Aggregation Inhibitors/isolation & purification MESH: Sequence Alignment MESH: Viper Venoms/chemistry* [SDV]Life Sciences [q-bio] |
spellingShingle |
Lebein Amino acid sequence Heterodimeric disintegrin MESH: Amino Acid Sequence MESH: Chromatography Gel MESH: Viper Venoms/isolation & purification MESH: Disintegrins/chemistry* MESH: Disintegrins/isolation & purification MESH: Mass Spectrometry MESH: Molecular Sequence Data MESH: Platelet Aggregation Inhibitors/chemistry* MESH: Platelet Aggregation Inhibitors/isolation & purification MESH: Sequence Alignment MESH: Viper Venoms/chemistry* [SDV]Life Sciences [q-bio] Gasmi, Ammar Srairi, Najet Guermazi, Sami Dkhil, Hafedh Karoui, Habib El Ayeb, Mohamed Amino acid structure and characterization of a heterodimeric disintegrin from Vipera lebetina venom |
topic_facet |
Lebein Amino acid sequence Heterodimeric disintegrin MESH: Amino Acid Sequence MESH: Chromatography Gel MESH: Viper Venoms/isolation & purification MESH: Disintegrins/chemistry* MESH: Disintegrins/isolation & purification MESH: Mass Spectrometry MESH: Molecular Sequence Data MESH: Platelet Aggregation Inhibitors/chemistry* MESH: Platelet Aggregation Inhibitors/isolation & purification MESH: Sequence Alignment MESH: Viper Venoms/chemistry* [SDV]Life Sciences [q-bio] |
description |
Referred to by :Ammar Gasmi, Najet Srairi, Sami Guermazi, Hafedh Dekhil, Habib Karoui, Mohamed ElAyebErratum to “Amino acid structure and characterization of a heterodimeric disintegrin from Vipera lebetina venom” [Biochim. Biophys. Acta 1547 (2001) 51–56]Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Volume 1764, Issue 9, September 2006, Pages 1525 International audience A heterodimeric disintegrin designed as lebein was isolated from crude Vipera lebetina venom using gel filtration, anion and cation exchange chromatographies on FPLC. The amino acid sequence of each subunit determined by Edman degradation contains 64 residues with ten half-cystines and an RGD site at the C-terminal part of the molecule. The molecular mass of native lebein determined by mass spectrometry was found to be 14 083.4 Da and those of α and β subunits were 6992.05 and 7117.62, respectively. These value are in good agreement with those calculated from the sequences. This protein strongly inhibits ADP induced platelet aggregation on human platelet rich plasma with IC50=160 nM. Sequences of this protein subunits displayed significant sequence similarities with many other monomeric and dimeric disintegrins reported from snake venoms. We identified an amino acid residue (N) in the hairpin loop of both subunits (CRARGDDMNDYC) which is different from all other reported motifs of disintegrins and this subtle difference may contribute to the distinct affinities and selectivities of this class of proteins. |
author2 |
Laboratoire des Venins et Toxines, Institut Pasteur de Tunis Institut Pasteur de Tunis Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) Laboratoire d'Hématologie, Institut Pasteur de Tunis The authors express their gratitude to P.Y. Haumont from Perkin-Elmer Biosystems (France) for the mass determination of lebein by mass spectrometry and to Dr. Z. Ben Lasfar for providing snake venom. |
format |
Article in Journal/Newspaper |
author |
Gasmi, Ammar Srairi, Najet Guermazi, Sami Dkhil, Hafedh Karoui, Habib El Ayeb, Mohamed |
author_facet |
Gasmi, Ammar Srairi, Najet Guermazi, Sami Dkhil, Hafedh Karoui, Habib El Ayeb, Mohamed |
author_sort |
Gasmi, Ammar |
title |
Amino acid structure and characterization of a heterodimeric disintegrin from Vipera lebetina venom |
title_short |
Amino acid structure and characterization of a heterodimeric disintegrin from Vipera lebetina venom |
title_full |
Amino acid structure and characterization of a heterodimeric disintegrin from Vipera lebetina venom |
title_fullStr |
Amino acid structure and characterization of a heterodimeric disintegrin from Vipera lebetina venom |
title_full_unstemmed |
Amino acid structure and characterization of a heterodimeric disintegrin from Vipera lebetina venom |
title_sort |
amino acid structure and characterization of a heterodimeric disintegrin from vipera lebetina venom |
publisher |
HAL CCSD |
publishDate |
2001 |
url |
https://hal-riip.archives-ouvertes.fr/pasteur-02026135 https://doi.org/10.1016/S0167-4838(01)00168-6 |
genre |
sami |
genre_facet |
sami |
op_source |
ISSN: 0167-4838 Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology https://hal-riip.archives-ouvertes.fr/pasteur-02026135 Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Elsevier, 2001, 1547 (1), pp.51-56. ⟨10.1016/S0167-4838(01)00168-6⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0167-4838(01)00168-6 info:eu-repo/semantics/altIdentifier/pmid/11343790 pasteur-02026135 https://hal-riip.archives-ouvertes.fr/pasteur-02026135 doi:10.1016/S0167-4838(01)00168-6 PUBMED: 11343790 |
op_doi |
https://doi.org/10.1016/S0167-4838(01)00168-6 |
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Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology |
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1547 |
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51 |
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56 |
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