Proteome modifications of juvenile beluga (Huso huso) brain as an effect of dietary methylmercury.
International audience Methylmercury (MeHg) is the most toxic form of mercury which is bioaccumulated in the aquatic food chain. It has been shown that one of the main targets of MeHg toxicity is the brain, but there is little knowledge of the molecular mechanisms of its toxic effects. In this work...
Published in: | Comparative Biochemistry and Physiology Part D: Genomics and Proteomics |
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ftccsdartic:oai:HAL:pasteur-00763511v1 2023-05-15T15:41:38+02:00 Proteome modifications of juvenile beluga (Huso huso) brain as an effect of dietary methylmercury. Keyvanshokooh, Saeed Vaziri, Behrouz Gharaei, Ahmad Mahboudi, Fereidoun Esmaili-Sari, Abbas Shahriari-Moghadam, Mohsen Department of Fisheries, Faculty of Marine Natural Resources Khorramshahr University of Marine Science and Technology Biotechnology Research Center Institut Pasteur d'Iran Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) Department of Fisheries, Faculty of Natural Resources Tarbiat Modares University Tehran Department of Environmental Science, Faculty of Natural Resources Zabol University This work has been supported by Tarbiat Modares University, Pasteur Institute of Iran, and Medical Biotechnology Network of Iran Saeed Keyvanshokooh Behrouz Vaziri Ahmad Gharaei Fereidoun Mahboudi Abbas Esmaili-Sari Mohsen Shahriari-Moghadam 2009-12 https://hal-riip.archives-ouvertes.fr/pasteur-00763511 https://hal-riip.archives-ouvertes.fr/pasteur-00763511/document https://hal-riip.archives-ouvertes.fr/pasteur-00763511/file/CBD136_2009.pdf https://doi.org/10.1016/j.cbd.2009.01.002 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbd.2009.01.002 info:eu-repo/semantics/altIdentifier/pmid/20403756 pasteur-00763511 https://hal-riip.archives-ouvertes.fr/pasteur-00763511 https://hal-riip.archives-ouvertes.fr/pasteur-00763511/document https://hal-riip.archives-ouvertes.fr/pasteur-00763511/file/CBD136_2009.pdf doi:10.1016/j.cbd.2009.01.002 PUBMED: 20403756 ISSN: 1744-117X Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics https://hal-riip.archives-ouvertes.fr/pasteur-00763511 Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, Elsevier, 2009, 4 (4), pp.243-8. ⟨10.1016/j.cbd.2009.01.002⟩ Methylmercury (MeHg) Proteomics Huso huso Brain Beluga MESH: Animals MESH: Brain MESH: Electrophoresis Gel Two-Dimensional MESH: Fishes MESH: Methylmercury Compounds MESH: Proteome [SDV.IMM]Life Sciences [q-bio]/Immunology info:eu-repo/semantics/article Journal articles 2009 ftccsdartic https://doi.org/10.1016/j.cbd.2009.01.002 2020-12-26T03:59:19Z International audience Methylmercury (MeHg) is the most toxic form of mercury which is bioaccumulated in the aquatic food chain. It has been shown that one of the main targets of MeHg toxicity is the brain, but there is little knowledge of the molecular mechanisms of its toxic effects. In this work we used a proteomics analysis to determine the changes in the brain proteome of juvenile beluga (Huso huso) exposed to dietary MeHg. The juvenile beluga were fed the diet containing 0.8 ppm MeHg for 70 days. Proteins of the brain tissue were analyzed using two-dimensional electrophoresis and MALDI-TOF/TOF mass spectrometry. We found eight proteins with significant altered expression level in the fish brain exposed to MeHg. These proteins are involved in different cell functions including cell metabolism, protein folding, cell division, and signal transduction. Our results support the idea that MeHg exerts its toxicity through oxidative stress induction and apoptotic effects. They also suggest that chronic MeHg exposure would induce an important metabolic deficiency in the brain. These findings provide basic information to understand possible mechanisms of MeHg toxicity in aquatic ecosystems. Article in Journal/Newspaper Beluga Beluga* Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Comparative Biochemistry and Physiology Part D: Genomics and Proteomics 4 4 243 248 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Methylmercury (MeHg) Proteomics Huso huso Brain Beluga MESH: Animals MESH: Brain MESH: Electrophoresis Gel Two-Dimensional MESH: Fishes MESH: Methylmercury Compounds MESH: Proteome [SDV.IMM]Life Sciences [q-bio]/Immunology |
spellingShingle |
Methylmercury (MeHg) Proteomics Huso huso Brain Beluga MESH: Animals MESH: Brain MESH: Electrophoresis Gel Two-Dimensional MESH: Fishes MESH: Methylmercury Compounds MESH: Proteome [SDV.IMM]Life Sciences [q-bio]/Immunology Keyvanshokooh, Saeed Vaziri, Behrouz Gharaei, Ahmad Mahboudi, Fereidoun Esmaili-Sari, Abbas Shahriari-Moghadam, Mohsen Proteome modifications of juvenile beluga (Huso huso) brain as an effect of dietary methylmercury. |
topic_facet |
Methylmercury (MeHg) Proteomics Huso huso Brain Beluga MESH: Animals MESH: Brain MESH: Electrophoresis Gel Two-Dimensional MESH: Fishes MESH: Methylmercury Compounds MESH: Proteome [SDV.IMM]Life Sciences [q-bio]/Immunology |
description |
International audience Methylmercury (MeHg) is the most toxic form of mercury which is bioaccumulated in the aquatic food chain. It has been shown that one of the main targets of MeHg toxicity is the brain, but there is little knowledge of the molecular mechanisms of its toxic effects. In this work we used a proteomics analysis to determine the changes in the brain proteome of juvenile beluga (Huso huso) exposed to dietary MeHg. The juvenile beluga were fed the diet containing 0.8 ppm MeHg for 70 days. Proteins of the brain tissue were analyzed using two-dimensional electrophoresis and MALDI-TOF/TOF mass spectrometry. We found eight proteins with significant altered expression level in the fish brain exposed to MeHg. These proteins are involved in different cell functions including cell metabolism, protein folding, cell division, and signal transduction. Our results support the idea that MeHg exerts its toxicity through oxidative stress induction and apoptotic effects. They also suggest that chronic MeHg exposure would induce an important metabolic deficiency in the brain. These findings provide basic information to understand possible mechanisms of MeHg toxicity in aquatic ecosystems. |
author2 |
Department of Fisheries, Faculty of Marine Natural Resources Khorramshahr University of Marine Science and Technology Biotechnology Research Center Institut Pasteur d'Iran Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) Department of Fisheries, Faculty of Natural Resources Tarbiat Modares University Tehran Department of Environmental Science, Faculty of Natural Resources Zabol University This work has been supported by Tarbiat Modares University, Pasteur Institute of Iran, and Medical Biotechnology Network of Iran Saeed Keyvanshokooh Behrouz Vaziri Ahmad Gharaei Fereidoun Mahboudi Abbas Esmaili-Sari Mohsen Shahriari-Moghadam |
format |
Article in Journal/Newspaper |
author |
Keyvanshokooh, Saeed Vaziri, Behrouz Gharaei, Ahmad Mahboudi, Fereidoun Esmaili-Sari, Abbas Shahriari-Moghadam, Mohsen |
author_facet |
Keyvanshokooh, Saeed Vaziri, Behrouz Gharaei, Ahmad Mahboudi, Fereidoun Esmaili-Sari, Abbas Shahriari-Moghadam, Mohsen |
author_sort |
Keyvanshokooh, Saeed |
title |
Proteome modifications of juvenile beluga (Huso huso) brain as an effect of dietary methylmercury. |
title_short |
Proteome modifications of juvenile beluga (Huso huso) brain as an effect of dietary methylmercury. |
title_full |
Proteome modifications of juvenile beluga (Huso huso) brain as an effect of dietary methylmercury. |
title_fullStr |
Proteome modifications of juvenile beluga (Huso huso) brain as an effect of dietary methylmercury. |
title_full_unstemmed |
Proteome modifications of juvenile beluga (Huso huso) brain as an effect of dietary methylmercury. |
title_sort |
proteome modifications of juvenile beluga (huso huso) brain as an effect of dietary methylmercury. |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://hal-riip.archives-ouvertes.fr/pasteur-00763511 https://hal-riip.archives-ouvertes.fr/pasteur-00763511/document https://hal-riip.archives-ouvertes.fr/pasteur-00763511/file/CBD136_2009.pdf https://doi.org/10.1016/j.cbd.2009.01.002 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_source |
ISSN: 1744-117X Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics https://hal-riip.archives-ouvertes.fr/pasteur-00763511 Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, Elsevier, 2009, 4 (4), pp.243-8. ⟨10.1016/j.cbd.2009.01.002⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbd.2009.01.002 info:eu-repo/semantics/altIdentifier/pmid/20403756 pasteur-00763511 https://hal-riip.archives-ouvertes.fr/pasteur-00763511 https://hal-riip.archives-ouvertes.fr/pasteur-00763511/document https://hal-riip.archives-ouvertes.fr/pasteur-00763511/file/CBD136_2009.pdf doi:10.1016/j.cbd.2009.01.002 PUBMED: 20403756 |
op_doi |
https://doi.org/10.1016/j.cbd.2009.01.002 |
container_title |
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics |
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4 |
container_issue |
4 |
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243 |
op_container_end_page |
248 |
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