Stanols as a tool to track the origin of microbial contamination of oysters, Crassostrea gigas, in shellfish areas.
International audience Runoff of cattle manures (cows, pigs, sheeps) or discharge of effluent from wastewater treatment plants (WWTP) into aquatic ecosystems can lead to microbiological contamination of waters and living organisms. In coastal ecosystems and particularly in shellfish harvesting areas...
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ftccsdartic:oai:HAL:insu-00853258v1 2023-05-15T15:58:27+02:00 Stanols as a tool to track the origin of microbial contamination of oysters, Crassostrea gigas, in shellfish areas. Harrault, Loïc Jardé, Emilie Jeanneau, Laurent Petitjean, Patrice Biogéochimie et écologie des milieux continentaux (Bioemco) Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Paris (ENS Paris) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL) Géosciences Rennes (GR) Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 1 (UR1) Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES) Vienne, Austria 2013-04 https://hal-insu.archives-ouvertes.fr/insu-00853258 en eng HAL CCSD insu-00853258 https://hal-insu.archives-ouvertes.fr/insu-00853258 BIBCODE: 2013EGUGA.15.4755H Geophysical Research Abstracts EGU General Assembly 2013 https://hal-insu.archives-ouvertes.fr/insu-00853258 EGU General Assembly 2013, Apr 2013, Vienne, Austria. pp.4755 [SDU.STU]Sciences of the Universe [physics]/Earth Sciences info:eu-repo/semantics/conferenceObject Conference papers 2013 ftccsdartic 2021-11-21T03:43:36Z International audience Runoff of cattle manures (cows, pigs, sheeps) or discharge of effluent from wastewater treatment plants (WWTP) into aquatic ecosystems can lead to microbiological contamination of waters and living organisms. In coastal ecosystems and particularly in shellfish harvesting areas, the presence of pathogen microorganisms in waters induces fecal contamination of filter feeding bivalves (oysters, mussels, scallops.), therefore leading to human health risks associated to the consumption of these contaminated organisms. Watershed management plans that aim at limiting these risks require the development of tools able to identify fecal contamination sources. The fecal indicator bacteria used in the regulations to determine fecal contamination are not source specific since they are found in the feces of most warm-blooded animals. Thus, microbiological biomarkers have been developed in association with chemical biomarkers as Microbial Source Tracking (MST) methods. Fecal stanols, by-products of sterols obtained by human and animal microbial gut flora, are found in considerable amounts in feces with different relative proportions depending on their animal or human source. Recently, in association with microbiological biomarkers, the stanol fingerprint of contaminated waters has been successfully used to determine the main source of fecal contamination (cow, pig or human sources) in rural watersheds (Brittany, France). Up to now, the use of the stanol fingerprint to track the fecal contamination in shellfish tissues, especially bivalves, has been limited to the analysis of coprostanol, a stanol commonly associated to human contamination. Therefore, whether the stanol fingerprint can be used as a MST method in bivalves or not is still unknown. The first aim of this study was to compare several organic extraction procedures of stanols in the oyster Crassostrea gigas to determine a reliable method for stanol fingerprint analysis in bivalves. Solvent extraction and purification steps have been carried out ... Conference Object Crassostrea gigas Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDU.STU]Sciences of the Universe [physics]/Earth Sciences |
spellingShingle |
[SDU.STU]Sciences of the Universe [physics]/Earth Sciences Harrault, Loïc Jardé, Emilie Jeanneau, Laurent Petitjean, Patrice Stanols as a tool to track the origin of microbial contamination of oysters, Crassostrea gigas, in shellfish areas. |
topic_facet |
[SDU.STU]Sciences of the Universe [physics]/Earth Sciences |
description |
International audience Runoff of cattle manures (cows, pigs, sheeps) or discharge of effluent from wastewater treatment plants (WWTP) into aquatic ecosystems can lead to microbiological contamination of waters and living organisms. In coastal ecosystems and particularly in shellfish harvesting areas, the presence of pathogen microorganisms in waters induces fecal contamination of filter feeding bivalves (oysters, mussels, scallops.), therefore leading to human health risks associated to the consumption of these contaminated organisms. Watershed management plans that aim at limiting these risks require the development of tools able to identify fecal contamination sources. The fecal indicator bacteria used in the regulations to determine fecal contamination are not source specific since they are found in the feces of most warm-blooded animals. Thus, microbiological biomarkers have been developed in association with chemical biomarkers as Microbial Source Tracking (MST) methods. Fecal stanols, by-products of sterols obtained by human and animal microbial gut flora, are found in considerable amounts in feces with different relative proportions depending on their animal or human source. Recently, in association with microbiological biomarkers, the stanol fingerprint of contaminated waters has been successfully used to determine the main source of fecal contamination (cow, pig or human sources) in rural watersheds (Brittany, France). Up to now, the use of the stanol fingerprint to track the fecal contamination in shellfish tissues, especially bivalves, has been limited to the analysis of coprostanol, a stanol commonly associated to human contamination. Therefore, whether the stanol fingerprint can be used as a MST method in bivalves or not is still unknown. The first aim of this study was to compare several organic extraction procedures of stanols in the oyster Crassostrea gigas to determine a reliable method for stanol fingerprint analysis in bivalves. Solvent extraction and purification steps have been carried out ... |
author2 |
Biogéochimie et écologie des milieux continentaux (Bioemco) Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Paris (ENS Paris) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL) Géosciences Rennes (GR) Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 1 (UR1) Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES) |
format |
Conference Object |
author |
Harrault, Loïc Jardé, Emilie Jeanneau, Laurent Petitjean, Patrice |
author_facet |
Harrault, Loïc Jardé, Emilie Jeanneau, Laurent Petitjean, Patrice |
author_sort |
Harrault, Loïc |
title |
Stanols as a tool to track the origin of microbial contamination of oysters, Crassostrea gigas, in shellfish areas. |
title_short |
Stanols as a tool to track the origin of microbial contamination of oysters, Crassostrea gigas, in shellfish areas. |
title_full |
Stanols as a tool to track the origin of microbial contamination of oysters, Crassostrea gigas, in shellfish areas. |
title_fullStr |
Stanols as a tool to track the origin of microbial contamination of oysters, Crassostrea gigas, in shellfish areas. |
title_full_unstemmed |
Stanols as a tool to track the origin of microbial contamination of oysters, Crassostrea gigas, in shellfish areas. |
title_sort |
stanols as a tool to track the origin of microbial contamination of oysters, crassostrea gigas, in shellfish areas. |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal-insu.archives-ouvertes.fr/insu-00853258 |
op_coverage |
Vienne, Austria |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Geophysical Research Abstracts EGU General Assembly 2013 https://hal-insu.archives-ouvertes.fr/insu-00853258 EGU General Assembly 2013, Apr 2013, Vienne, Austria. pp.4755 |
op_relation |
insu-00853258 https://hal-insu.archives-ouvertes.fr/insu-00853258 BIBCODE: 2013EGUGA.15.4755H |
_version_ |
1766394194293686272 |