Functional significance of genotoxicity in fish germ cells
The aquatic environment is becoming increasingly contaminated by pollutants having a genotoxic potential towards organisms and in particular in fish. Such genotoxins are prone to affect directly offspring or indirectly through the reproductive process. All this could influence recruitment rate and h...
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ftccsdartic:oai:HAL:ineris-00973575v1 2023-05-15T14:30:11+02:00 Functional significance of genotoxicity in fish germ cells Devaux, Alain Gillet, Christian Fiat, Luc Santos, Raphaël Sanchez, Wilfried Bony, Sylvie Laboratoire des Sciences de l'Environnement École Nationale des Travaux Publics de l'État (ENTPE) Centre Alpin de Recherche sur les Réseaux Trophiques et Ecosystèmes Limniques (CARRTEL) Institut National de la Recherche Agronomique (INRA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry ) Institut National de l'Environnement Industriel et des Risques (INERIS) Séville, Spain 2010-05-23 https://hal-ineris.archives-ouvertes.fr/ineris-00973575 https://hal-ineris.archives-ouvertes.fr/ineris-00973575/document https://hal-ineris.archives-ouvertes.fr/ineris-00973575/file/2010-136_hal.pdf en eng HAL CCSD ineris-00973575 https://hal-ineris.archives-ouvertes.fr/ineris-00973575 https://hal-ineris.archives-ouvertes.fr/ineris-00973575/document https://hal-ineris.archives-ouvertes.fr/ineris-00973575/file/2010-136_hal.pdf INERIS: EN-2010-136 info:eu-repo/semantics/OpenAccess 20. SETAC Europe Annual Meeting https://hal-ineris.archives-ouvertes.fr/ineris-00973575 20. SETAC Europe Annual Meeting, May 2010, Séville, Spain FISH GENOTOXICITY REPRODUCTION SPERM [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology [SDE]Environmental Sciences info:eu-repo/semantics/conferenceObject Conference papers 2010 ftccsdartic 2021-07-04T01:41:43Z The aquatic environment is becoming increasingly contaminated by pollutants having a genotoxic potential towards organisms and in particular in fish. Such genotoxins are prone to affect directly offspring or indirectly through the reproductive process. All this could influence recruitment rate and hence the population dynamics. However, assessment of the ecological risks associated with environmental genotoxic exposure is usually based on individual responses. Thus, there is a need for a better understanding of the long term and population level implications of genotoxic insults in fish. While low levels of DNA damage in somatic cells and oocytes can be efficiently repaired, mature sperm cells, i.e. spermatozoa, are susceptible to accumulate damage due to their lack of repair capacity. The present work aims to track the transfer of toxic effects across generations by studying the link between the level of DNA damage in fish sperm, and the rate of development abnormalities measured in the offspring after parental exposure to the model genotoxicant MMS. Three different fish species were chosen based either on their ecological importance or on their reproduction behavior, respectively brown trout (Salmo trutta), Arctic charr (Salvelinus alpinus) and threespine stickleback (Gasterosteus aculeatus). Results show a significant increase in sperm DNA damage measured with the comet assay in exposed organisms. This damage did not impact on fertilization success but led further to a significant increase in embryo abnormality rate at early embryonic and late larval stages, and further delayed growth in exposed group compared to the control. Conference Object Arctic charr Arctic Salvelinus alpinus Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Arctic |
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Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
FISH GENOTOXICITY REPRODUCTION SPERM [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology [SDE]Environmental Sciences |
spellingShingle |
FISH GENOTOXICITY REPRODUCTION SPERM [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology [SDE]Environmental Sciences Devaux, Alain Gillet, Christian Fiat, Luc Santos, Raphaël Sanchez, Wilfried Bony, Sylvie Functional significance of genotoxicity in fish germ cells |
topic_facet |
FISH GENOTOXICITY REPRODUCTION SPERM [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology [SDE]Environmental Sciences |
description |
The aquatic environment is becoming increasingly contaminated by pollutants having a genotoxic potential towards organisms and in particular in fish. Such genotoxins are prone to affect directly offspring or indirectly through the reproductive process. All this could influence recruitment rate and hence the population dynamics. However, assessment of the ecological risks associated with environmental genotoxic exposure is usually based on individual responses. Thus, there is a need for a better understanding of the long term and population level implications of genotoxic insults in fish. While low levels of DNA damage in somatic cells and oocytes can be efficiently repaired, mature sperm cells, i.e. spermatozoa, are susceptible to accumulate damage due to their lack of repair capacity. The present work aims to track the transfer of toxic effects across generations by studying the link between the level of DNA damage in fish sperm, and the rate of development abnormalities measured in the offspring after parental exposure to the model genotoxicant MMS. Three different fish species were chosen based either on their ecological importance or on their reproduction behavior, respectively brown trout (Salmo trutta), Arctic charr (Salvelinus alpinus) and threespine stickleback (Gasterosteus aculeatus). Results show a significant increase in sperm DNA damage measured with the comet assay in exposed organisms. This damage did not impact on fertilization success but led further to a significant increase in embryo abnormality rate at early embryonic and late larval stages, and further delayed growth in exposed group compared to the control. |
author2 |
Laboratoire des Sciences de l'Environnement École Nationale des Travaux Publics de l'État (ENTPE) Centre Alpin de Recherche sur les Réseaux Trophiques et Ecosystèmes Limniques (CARRTEL) Institut National de la Recherche Agronomique (INRA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry ) Institut National de l'Environnement Industriel et des Risques (INERIS) |
format |
Conference Object |
author |
Devaux, Alain Gillet, Christian Fiat, Luc Santos, Raphaël Sanchez, Wilfried Bony, Sylvie |
author_facet |
Devaux, Alain Gillet, Christian Fiat, Luc Santos, Raphaël Sanchez, Wilfried Bony, Sylvie |
author_sort |
Devaux, Alain |
title |
Functional significance of genotoxicity in fish germ cells |
title_short |
Functional significance of genotoxicity in fish germ cells |
title_full |
Functional significance of genotoxicity in fish germ cells |
title_fullStr |
Functional significance of genotoxicity in fish germ cells |
title_full_unstemmed |
Functional significance of genotoxicity in fish germ cells |
title_sort |
functional significance of genotoxicity in fish germ cells |
publisher |
HAL CCSD |
publishDate |
2010 |
url |
https://hal-ineris.archives-ouvertes.fr/ineris-00973575 https://hal-ineris.archives-ouvertes.fr/ineris-00973575/document https://hal-ineris.archives-ouvertes.fr/ineris-00973575/file/2010-136_hal.pdf |
op_coverage |
Séville, Spain |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Salvelinus alpinus |
op_source |
20. SETAC Europe Annual Meeting https://hal-ineris.archives-ouvertes.fr/ineris-00973575 20. SETAC Europe Annual Meeting, May 2010, Séville, Spain |
op_relation |
ineris-00973575 https://hal-ineris.archives-ouvertes.fr/ineris-00973575 https://hal-ineris.archives-ouvertes.fr/ineris-00973575/document https://hal-ineris.archives-ouvertes.fr/ineris-00973575/file/2010-136_hal.pdf INERIS: EN-2010-136 |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1766304085016838144 |