Ontogeny of muscle bioenergetics in Adelie penguin chicks (Pygoscelis adeliae)
International audience The ontogeny of pectoralis muscle bioenergetics was studied in growing Adelie penguin chicks during the first month after hatching and compared with adults using permeabilized fibers and isolated mitochondria. With pyruvatemalate-succinate or palmitoyl-carnitine as substrates,...
Published in: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
---|---|
Main Authors: | , , , , , , |
Other Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2013
|
Subjects: | |
Online Access: | https://hal.archives-ouvertes.fr/halsde-00942580 https://doi.org/10.1152/ajpregu.00137.2013 |
id |
ftccsdartic:oai:HAL:halsde-00942580v1 |
---|---|
record_format |
openpolar |
spelling |
ftccsdartic:oai:HAL:halsde-00942580v1 2023-05-15T13:04:46+02:00 Ontogeny of muscle bioenergetics in Adelie penguin chicks (Pygoscelis adeliae) Fongy, Anaïs Romestaing, Caroline Blanc,, Coralie Lacoste-Garanger,, Nicolas Rouanet, Jean-Louis Raccurt, Mireille Duchamp, Claude Écophysiologie, Comportement, Conservation Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA) Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS) 2013 https://hal.archives-ouvertes.fr/halsde-00942580 https://doi.org/10.1152/ajpregu.00137.2013 en eng HAL CCSD American Physiological Society info:eu-repo/semantics/altIdentifier/doi/10.1152/ajpregu.00137.2013 halsde-00942580 https://hal.archives-ouvertes.fr/halsde-00942580 doi:10.1152/ajpregu.00137.2013 ISSN: 0363-6119 EISSN: 1522-1490 AJP - Regulatory, Integrative and Comparative Physiology https://hal.archives-ouvertes.fr/halsde-00942580 AJP - Regulatory, Integrative and Comparative Physiology, American Physiological Society, 2013, 305 (9), pp.1065-1075. ⟨10.1152/ajpregu.00137.2013⟩ [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2013 ftccsdartic https://doi.org/10.1152/ajpregu.00137.2013 2021-12-19T03:09:04Z International audience The ontogeny of pectoralis muscle bioenergetics was studied in growing Adelie penguin chicks during the first month after hatching and compared with adults using permeabilized fibers and isolated mitochondria. With pyruvatemalate-succinate or palmitoyl-carnitine as substrates, permeabilized fiber respiration markedly increased during chick growth (3-fold) and further rose in adults (1.4-fold). Several markers of muscle fiber oxidative activity (cytochrome oxidase, citrate synthase, hydroxylacyl-CoA dehydrogenase) increased 6- to 19-fold with age together with large rises in intermyofibrillar (IMF) and subsarcolemmal (SS) mitochondrial content (3- to 5-fold) and oxidative activities (1.5- to 2.4-fold). The proportion of IMF relative to SS mitochondria increased with chick age but markedly dropped in adults. Differences in oxidative activity between mitochondrial fractions were reduced in adults compared with hatched chicks. Extrapolation of mitochondrial to muscle respirations revealed similar figures with isolated mitochondria and permeabilized fibers with carbohydrate-derived but not with lipid-derived substrates, suggesting diffusion limitations of lipid substrates with permeabilized fibers. Two immunoreactive fusion proteins, mitofusin 2 (Mfn2) and optic atrophy 1 (OPA1), were detected by Western blots on mitochondrial extracts and their relative abundance increased with age. Muscle fiber respiration was positively related with Mfn2 and OPA1 relative abundance. Present data showed by two complementary techniques large ontogenic increases in muscle oxidative activity that may enable birds to face thermal emancipation and growth in childhood and marine life in adulthood. The concomitant rise in mitochondrial fusion protein abundance suggests a role of mitochondrial networks in the skeletal muscle processes of bioenergetics that enable penguins to overcome harsh environmental constraints. Article in Journal/Newspaper Adelie penguin Pygoscelis adeliae Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 305 9 R1065 R1075 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
[SDE.BE]Environmental Sciences/Biodiversity and Ecology Fongy, Anaïs Romestaing, Caroline Blanc,, Coralie Lacoste-Garanger,, Nicolas Rouanet, Jean-Louis Raccurt, Mireille Duchamp, Claude Ontogeny of muscle bioenergetics in Adelie penguin chicks (Pygoscelis adeliae) |
topic_facet |
[SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience The ontogeny of pectoralis muscle bioenergetics was studied in growing Adelie penguin chicks during the first month after hatching and compared with adults using permeabilized fibers and isolated mitochondria. With pyruvatemalate-succinate or palmitoyl-carnitine as substrates, permeabilized fiber respiration markedly increased during chick growth (3-fold) and further rose in adults (1.4-fold). Several markers of muscle fiber oxidative activity (cytochrome oxidase, citrate synthase, hydroxylacyl-CoA dehydrogenase) increased 6- to 19-fold with age together with large rises in intermyofibrillar (IMF) and subsarcolemmal (SS) mitochondrial content (3- to 5-fold) and oxidative activities (1.5- to 2.4-fold). The proportion of IMF relative to SS mitochondria increased with chick age but markedly dropped in adults. Differences in oxidative activity between mitochondrial fractions were reduced in adults compared with hatched chicks. Extrapolation of mitochondrial to muscle respirations revealed similar figures with isolated mitochondria and permeabilized fibers with carbohydrate-derived but not with lipid-derived substrates, suggesting diffusion limitations of lipid substrates with permeabilized fibers. Two immunoreactive fusion proteins, mitofusin 2 (Mfn2) and optic atrophy 1 (OPA1), were detected by Western blots on mitochondrial extracts and their relative abundance increased with age. Muscle fiber respiration was positively related with Mfn2 and OPA1 relative abundance. Present data showed by two complementary techniques large ontogenic increases in muscle oxidative activity that may enable birds to face thermal emancipation and growth in childhood and marine life in adulthood. The concomitant rise in mitochondrial fusion protein abundance suggests a role of mitochondrial networks in the skeletal muscle processes of bioenergetics that enable penguins to overcome harsh environmental constraints. |
author2 |
Écophysiologie, Comportement, Conservation Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA) Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Fongy, Anaïs Romestaing, Caroline Blanc,, Coralie Lacoste-Garanger,, Nicolas Rouanet, Jean-Louis Raccurt, Mireille Duchamp, Claude |
author_facet |
Fongy, Anaïs Romestaing, Caroline Blanc,, Coralie Lacoste-Garanger,, Nicolas Rouanet, Jean-Louis Raccurt, Mireille Duchamp, Claude |
author_sort |
Fongy, Anaïs |
title |
Ontogeny of muscle bioenergetics in Adelie penguin chicks (Pygoscelis adeliae) |
title_short |
Ontogeny of muscle bioenergetics in Adelie penguin chicks (Pygoscelis adeliae) |
title_full |
Ontogeny of muscle bioenergetics in Adelie penguin chicks (Pygoscelis adeliae) |
title_fullStr |
Ontogeny of muscle bioenergetics in Adelie penguin chicks (Pygoscelis adeliae) |
title_full_unstemmed |
Ontogeny of muscle bioenergetics in Adelie penguin chicks (Pygoscelis adeliae) |
title_sort |
ontogeny of muscle bioenergetics in adelie penguin chicks (pygoscelis adeliae) |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.archives-ouvertes.fr/halsde-00942580 https://doi.org/10.1152/ajpregu.00137.2013 |
genre |
Adelie penguin Pygoscelis adeliae |
genre_facet |
Adelie penguin Pygoscelis adeliae |
op_source |
ISSN: 0363-6119 EISSN: 1522-1490 AJP - Regulatory, Integrative and Comparative Physiology https://hal.archives-ouvertes.fr/halsde-00942580 AJP - Regulatory, Integrative and Comparative Physiology, American Physiological Society, 2013, 305 (9), pp.1065-1075. ⟨10.1152/ajpregu.00137.2013⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1152/ajpregu.00137.2013 halsde-00942580 https://hal.archives-ouvertes.fr/halsde-00942580 doi:10.1152/ajpregu.00137.2013 |
op_doi |
https://doi.org/10.1152/ajpregu.00137.2013 |
container_title |
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
container_volume |
305 |
container_issue |
9 |
container_start_page |
R1065 |
op_container_end_page |
R1075 |
_version_ |
1766370481934434304 |