Bacterial Diversity of Terrestrial Crystalline Volcanic Rocks, Iceland

• Published in: Microbial Ecology
• Main Authors: C. Kelly, Laura, S. Cockell, Charles, Herrera-Belaroussi, Aude, Piceno, Yvette, Andersen, Gary, Desantis, Todd, Brodie, Eoin, Thorsteinsson, Thorsteinn, Marteinsson, Viggó, Poly, Franck, Leroux, Xavier
• Other Authors: Geomicrobiology Research Group Milton Keynes, Planetary and Space Sciences Research Institute Milton Keynes (PSSRI), Centre for Earth, Planetary, Space and Astronomical Research Milton Keynes (CEPSAR), The Open University Milton Keynes (OU)-The Open University Milton Keynes (OU)-Centre for Earth, Planetary, Space and Astronomical Research Milton Keynes (CEPSAR), The Open University Milton Keynes (OU)-The Open University Milton Keynes (OU), Ecology Department LBNL Berkeley, Earth Science Division LBNL Berkeley (ESD), Lawrence Berkeley National Laboratory Berkeley (LBNL)-Lawrence Berkeley National Laboratory Berkeley (LBNL), Hydrology Division, National Energy Authority, Matís ohf./Icelandic Food and Biotech R&D, Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Leverhulme Trust F/00 269/N
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2011
• Subjects: MONOXIDE-OXIDIZING BACTERIA; CLONE LIBRARY; MICROBIAL DIVERSITY; COMMUNITY STRUCTURE; BASALTIC GLASS; FLOOR BASALT; MICROARRAY; POPULATIONS; DEPOSITS; DYNAMICS; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; Antarctic; Dómadalshraun; Antarc*; Iceland
• Online Access: https://hal.science/halsde-00723396; https://doi.org/10.1007/s00248-011-9864-1

International audience Bacteria inhabiting crystalline rocks from two terrestrial Icelandic volcanic lava flows of similar age and from the same geographical region, but differing in porosity and mineralogy, were characterised. Microarray (PhyloChip) and clone library analysis of 16S rRNA genes revealed the presence of a diverse assemblage of bacteria in each lava flow. Both methods suggested a more diverse community at the Dómadalshraun site (rhyolitic/andesitic lava flow) than that present at the Hnausahraun site (basaltic lava flow). Proteobacteria dominated the clone library at the Dómadalshraun site, while Acidobacteria was the most abundant phylum in the Hnausahraun site. Although analysis of similarities of denaturing gradient gel electrophoresis profiles suggested a strong correlation of community structure with mineralogy, rock porosity may also play an important role in shaping the bacterial community in crystalline volcanic rocks. Clone sequences were most similar to uncultured microorganisms, mainly from soil environments. Of these, Antarctic soils and temperate rhizosphere soils were prominent, as were clones retrieved from Hawaiian and Andean volcanic soils. The novel diversity of these Icelandic microbial communities was supported by the finding that up to 46% of clones displayed <85% sequence identities to sequences currently deposited in the RDP database