Improved noninvasive genotyping method: application to brown bear (Ursus arctos) faeces

• Published in: Molecular Ecology Notes
• Main Authors: Bellemain, E., Taberlet, P.
• Other Authors: Laboratoire d'Ecologie Alpine (LECA), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry ), Department of Ecology and Natural Resource Management, Norwegian University of Life Sciences (NMBU)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2004
• Subjects: microsatellites; multiplex preamplification method; noninvasive samples; sex-specific primer; Ursus arctos; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; [SDV.BID]Life Sciences [q-bio]/Biodiversity; [SDV.EE]Life Sciences [q-bio]/Ecology; environment
• Online Access: https://hal.archives-ouvertes.fr/halsde-00279779; https://doi.org/10.1111/j.1471-8286.2004.00711.x

Times Cited: 13 International audience We redesigned new microsatellite primers and one sex-specific primer for amplification of faecal DNA from brown bears (Ursus arctos). We also combined a semi-nested polymerase chain reaction (PCR) with a newly developed multiplex preamplification method in order to increase the quality of the amplified DNA fragments. In comparison with a conventional PCR approach, the genotyping error rate was substantially reduced and the amplification rate was increased. This new approach could be transposed to other species where conventional PCR methods experience low success due to limited DNA concentration and/or quality.