Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique
International audience Background: Ticks are primary vectors for many well-known disease-causing agents that affect human and animal populations globally such as tick-borne encephalitis, Crimean-Congo hemorrhagic fever and African swine fever. In this study, viral metagenomics was used to identify w...
Published in: | Infection Ecology & Epidemiology |
---|---|
Main Authors: | , , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2018
|
Subjects: | |
Online Access: | https://hal.science/hal-04406520 https://hal.science/hal-04406520/document https://hal.science/hal-04406520/file/2018_InfectionEcologyEpidemio_ViralMetagenomics-ticks-Mozambique.pdf https://doi.org/10.1080/20008686.2018.1478585 |
id |
ftccsdartic:oai:HAL:hal-04406520v1 |
---|---|
record_format |
openpolar |
spelling |
ftccsdartic:oai:HAL:hal-04406520v1 2024-02-27T08:39:45+00:00 Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique Cholleti, Harindranath Hayer, Juliette Mulandane, Fernando, Chanisso Falk, Kerstin Fafetine, Jose Berg, Mikael Blomström, Anne-Lie Swedish University of Agricultural Sciences (SLU) 2018-05-28 https://hal.science/hal-04406520 https://hal.science/hal-04406520/document https://hal.science/hal-04406520/file/2018_InfectionEcologyEpidemio_ViralMetagenomics-ticks-Mozambique.pdf https://doi.org/10.1080/20008686.2018.1478585 en eng HAL CCSD Taylor & Francis info:eu-repo/semantics/altIdentifier/doi/10.1080/20008686.2018.1478585 hal-04406520 https://hal.science/hal-04406520 https://hal.science/hal-04406520/document https://hal.science/hal-04406520/file/2018_InfectionEcologyEpidemio_ViralMetagenomics-ticks-Mozambique.pdf doi:10.1080/20008686.2018.1478585 info:eu-repo/semantics/OpenAccess ISSN: 2000-8686 Infection Ecology & Epidemiology https://hal.science/hal-04406520 Infection Ecology & Epidemiology, 2018, 8 (1), pp.1478585. ⟨10.1080/20008686.2018.1478585⟩ Ticks arthropods Rhipicephalus quaranjavirus viral metagenomics Mozambique Ticks arthropods Rhipicephalus quaranjavirus viral metagenomics Mozambique [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2018 ftccsdartic https://doi.org/10.1080/20008686.2018.1478585 2024-01-28T00:03:20Z International audience Background: Ticks are primary vectors for many well-known disease-causing agents that affect human and animal populations globally such as tick-borne encephalitis, Crimean-Congo hemorrhagic fever and African swine fever. In this study, viral metagenomics was used to identify what viruses are present in Rhipicephalus spp. ticks collected in the Zambezi Valley of Mozambique. Methods: The RNA was amplified with sequence-independent single primer amplification (SISPA) and high-throughput sequencing was performed on the Ion Torrent platform. The generated sequences were subjected to quality check and classfied by BLAST. CodonCode aligner and SeqMan were used to assemble the sequences. Results: The majority of viral sequences showed closest sequence identity to the Orthomyxoviridae family, although viruses similar to the Parvoviridae and Coronaviridae were also identified. Nearly complete sequences of five orthomyxoviral segments (HA, NP, PB1, PB2, and PA) were obtained and these showed an amino acid identity of 32-52% to known quaranjaviruses. The sequences were most closely related to the Wellfleet Bay virus, detected and isolated from common eider during a mortality event in the USA. Conclusions: In summary, this study has identified a highly divergent virus with in the Orthomyxoviridae family associated with Rhipicephalus ticks from Mozambique. Further genetic and biological studies are needed in order to investigate potential pathogenesis of the identified orthomyxovirus. Article in Journal/Newspaper Common Eider Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Infection Ecology & Epidemiology 8 1 1478585 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Ticks arthropods Rhipicephalus quaranjavirus viral metagenomics Mozambique Ticks arthropods Rhipicephalus quaranjavirus viral metagenomics Mozambique [SDV]Life Sciences [q-bio] |
spellingShingle |
Ticks arthropods Rhipicephalus quaranjavirus viral metagenomics Mozambique Ticks arthropods Rhipicephalus quaranjavirus viral metagenomics Mozambique [SDV]Life Sciences [q-bio] Cholleti, Harindranath Hayer, Juliette Mulandane, Fernando, Chanisso Falk, Kerstin Fafetine, Jose Berg, Mikael Blomström, Anne-Lie Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
topic_facet |
Ticks arthropods Rhipicephalus quaranjavirus viral metagenomics Mozambique Ticks arthropods Rhipicephalus quaranjavirus viral metagenomics Mozambique [SDV]Life Sciences [q-bio] |
description |
International audience Background: Ticks are primary vectors for many well-known disease-causing agents that affect human and animal populations globally such as tick-borne encephalitis, Crimean-Congo hemorrhagic fever and African swine fever. In this study, viral metagenomics was used to identify what viruses are present in Rhipicephalus spp. ticks collected in the Zambezi Valley of Mozambique. Methods: The RNA was amplified with sequence-independent single primer amplification (SISPA) and high-throughput sequencing was performed on the Ion Torrent platform. The generated sequences were subjected to quality check and classfied by BLAST. CodonCode aligner and SeqMan were used to assemble the sequences. Results: The majority of viral sequences showed closest sequence identity to the Orthomyxoviridae family, although viruses similar to the Parvoviridae and Coronaviridae were also identified. Nearly complete sequences of five orthomyxoviral segments (HA, NP, PB1, PB2, and PA) were obtained and these showed an amino acid identity of 32-52% to known quaranjaviruses. The sequences were most closely related to the Wellfleet Bay virus, detected and isolated from common eider during a mortality event in the USA. Conclusions: In summary, this study has identified a highly divergent virus with in the Orthomyxoviridae family associated with Rhipicephalus ticks from Mozambique. Further genetic and biological studies are needed in order to investigate potential pathogenesis of the identified orthomyxovirus. |
author2 |
Swedish University of Agricultural Sciences (SLU) |
format |
Article in Journal/Newspaper |
author |
Cholleti, Harindranath Hayer, Juliette Mulandane, Fernando, Chanisso Falk, Kerstin Fafetine, Jose Berg, Mikael Blomström, Anne-Lie |
author_facet |
Cholleti, Harindranath Hayer, Juliette Mulandane, Fernando, Chanisso Falk, Kerstin Fafetine, Jose Berg, Mikael Blomström, Anne-Lie |
author_sort |
Cholleti, Harindranath |
title |
Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
title_short |
Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
title_full |
Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
title_fullStr |
Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
title_full_unstemmed |
Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique |
title_sort |
viral metagenomics reveals the presence of highly divergent quaranjavirus in rhipicephalus ticks from mozambique |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.science/hal-04406520 https://hal.science/hal-04406520/document https://hal.science/hal-04406520/file/2018_InfectionEcologyEpidemio_ViralMetagenomics-ticks-Mozambique.pdf https://doi.org/10.1080/20008686.2018.1478585 |
genre |
Common Eider |
genre_facet |
Common Eider |
op_source |
ISSN: 2000-8686 Infection Ecology & Epidemiology https://hal.science/hal-04406520 Infection Ecology & Epidemiology, 2018, 8 (1), pp.1478585. ⟨10.1080/20008686.2018.1478585⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1080/20008686.2018.1478585 hal-04406520 https://hal.science/hal-04406520 https://hal.science/hal-04406520/document https://hal.science/hal-04406520/file/2018_InfectionEcologyEpidemio_ViralMetagenomics-ticks-Mozambique.pdf doi:10.1080/20008686.2018.1478585 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1080/20008686.2018.1478585 |
container_title |
Infection Ecology & Epidemiology |
container_volume |
8 |
container_issue |
1 |
container_start_page |
1478585 |
_version_ |
1792046799254454272 |