The combined effect of stress and diazepam on facultative migration of European glass eel (Anguilla anguilla): development of endocrine and molecular markers
International audience The European eel (Anguilla anguilla) population has received considerable attention in recent decades due to its significant decline. This catadromous species presents a facultative diadromy and glass eels display a large panel of estuarine migratory tactics, ranging from resi...
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ftccsdartic:oai:HAL:hal-04238376v1 2023-12-10T09:39:46+01:00 The combined effect of stress and diazepam on facultative migration of European glass eel (Anguilla anguilla): development of endocrine and molecular markers Sebihi, Stellia Morère, Marie Bouchard, Colin Coste-Heinrich, Pascale Monperrus, Mathilde Ortiz-Zarragoitia, Maren Bolliet, Valérie Ecologie Comportementale et Biologie des Populations de Poissons (ECOBIOP) Université de Pau et des Pays de l'Adour (UPPA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM) Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) Dublin (IR), Ireland 2023-04-30 https://univ-pau.hal.science/hal-04238376 en eng HAL CCSD hal-04238376 https://univ-pau.hal.science/hal-04238376 SETAC Europe 33rd Annual Meeting https://univ-pau.hal.science/hal-04238376 SETAC Europe 33rd Annual Meeting, Apr 2023, Dublin (IR), Ireland [CHIM]Chemical Sciences [SDE]Environmental Sciences info:eu-repo/semantics/conferenceObject Conference poster 2023 ftccsdartic 2023-11-11T23:45:24Z International audience The European eel (Anguilla anguilla) population has received considerable attention in recent decades due to its significant decline. This catadromous species presents a facultative diadromy and glass eels display a large panel of estuarine migratory tactics, ranging from residency in marine water, to various degrees of upstream colonization through estuarine and freshwater ecosystems. These different patterns of migration can have a strong impact on the fate of the population because individuals remaining downstream mostly develop into males, whereas individuals colonizing upstream develop mainly into females. Recent results suggest that glass eels presenting a low probability to migrate in experimental facilities may be more stressed or more sensitive to stress than migrant individuals. Estuaries are considered stressful environments and represent a major sink for variouscontaminants usually considered as powerful stressors depending on their concentrations. Among these chemicals, benzodiazepines, found in relatively high concentrations in effluents of wastewater treatment plants, can induce a number of neuroendocrine alterations in exposed fish. However, paradoxically, they also may counteract the stress responses in fish due to their anxiolytic effect.The aim of this work was to investigate the effect of the stress and diazepam at environmental doses on the European glass eel by developing stress markers. Stress hormones analysis were developed by LC-MS-MS to determine cortisol, cortisone, 11- deoxycortisol, and 11-deoxycorticosterone production in glass eel individuals. In addition, transcriptomic markers were detected using a non-targeted approach, by RNAseq. These molecular and endocrine markers were analyzed on glass eels contaminated or not with diazepam, and exposed or not to a 2 minutes mechanical stress. Both stress markers were monitored with time (after 15, 30, 60 and 120 min) to evaluate the combined effect of stress and diazepam in glass eel. The developed stress markers ... Conference Object Anguilla anguilla European eel Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[CHIM]Chemical Sciences [SDE]Environmental Sciences |
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[CHIM]Chemical Sciences [SDE]Environmental Sciences Sebihi, Stellia Morère, Marie Bouchard, Colin Coste-Heinrich, Pascale Monperrus, Mathilde Ortiz-Zarragoitia, Maren Bolliet, Valérie The combined effect of stress and diazepam on facultative migration of European glass eel (Anguilla anguilla): development of endocrine and molecular markers |
topic_facet |
[CHIM]Chemical Sciences [SDE]Environmental Sciences |
description |
International audience The European eel (Anguilla anguilla) population has received considerable attention in recent decades due to its significant decline. This catadromous species presents a facultative diadromy and glass eels display a large panel of estuarine migratory tactics, ranging from residency in marine water, to various degrees of upstream colonization through estuarine and freshwater ecosystems. These different patterns of migration can have a strong impact on the fate of the population because individuals remaining downstream mostly develop into males, whereas individuals colonizing upstream develop mainly into females. Recent results suggest that glass eels presenting a low probability to migrate in experimental facilities may be more stressed or more sensitive to stress than migrant individuals. Estuaries are considered stressful environments and represent a major sink for variouscontaminants usually considered as powerful stressors depending on their concentrations. Among these chemicals, benzodiazepines, found in relatively high concentrations in effluents of wastewater treatment plants, can induce a number of neuroendocrine alterations in exposed fish. However, paradoxically, they also may counteract the stress responses in fish due to their anxiolytic effect.The aim of this work was to investigate the effect of the stress and diazepam at environmental doses on the European glass eel by developing stress markers. Stress hormones analysis were developed by LC-MS-MS to determine cortisol, cortisone, 11- deoxycortisol, and 11-deoxycorticosterone production in glass eel individuals. In addition, transcriptomic markers were detected using a non-targeted approach, by RNAseq. These molecular and endocrine markers were analyzed on glass eels contaminated or not with diazepam, and exposed or not to a 2 minutes mechanical stress. Both stress markers were monitored with time (after 15, 30, 60 and 120 min) to evaluate the combined effect of stress and diazepam in glass eel. The developed stress markers ... |
author2 |
Ecologie Comportementale et Biologie des Populations de Poissons (ECOBIOP) Université de Pau et des Pays de l'Adour (UPPA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM) Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Conference Object |
author |
Sebihi, Stellia Morère, Marie Bouchard, Colin Coste-Heinrich, Pascale Monperrus, Mathilde Ortiz-Zarragoitia, Maren Bolliet, Valérie |
author_facet |
Sebihi, Stellia Morère, Marie Bouchard, Colin Coste-Heinrich, Pascale Monperrus, Mathilde Ortiz-Zarragoitia, Maren Bolliet, Valérie |
author_sort |
Sebihi, Stellia |
title |
The combined effect of stress and diazepam on facultative migration of European glass eel (Anguilla anguilla): development of endocrine and molecular markers |
title_short |
The combined effect of stress and diazepam on facultative migration of European glass eel (Anguilla anguilla): development of endocrine and molecular markers |
title_full |
The combined effect of stress and diazepam on facultative migration of European glass eel (Anguilla anguilla): development of endocrine and molecular markers |
title_fullStr |
The combined effect of stress and diazepam on facultative migration of European glass eel (Anguilla anguilla): development of endocrine and molecular markers |
title_full_unstemmed |
The combined effect of stress and diazepam on facultative migration of European glass eel (Anguilla anguilla): development of endocrine and molecular markers |
title_sort |
combined effect of stress and diazepam on facultative migration of european glass eel (anguilla anguilla): development of endocrine and molecular markers |
publisher |
HAL CCSD |
publishDate |
2023 |
url |
https://univ-pau.hal.science/hal-04238376 |
op_coverage |
Dublin (IR), Ireland |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
SETAC Europe 33rd Annual Meeting https://univ-pau.hal.science/hal-04238376 SETAC Europe 33rd Annual Meeting, Apr 2023, Dublin (IR), Ireland |
op_relation |
hal-04238376 https://univ-pau.hal.science/hal-04238376 |
_version_ |
1784888891113734144 |