Photoacclimation of the polar diatom Chaetoceros neogracilis at low temperature
Polar microalgae face two major challenges: 1- growing at temperatures (-1.7 to 5°C) that limit enzyme kinetics; and 2- surviving and exploiting a wide range of irradiance. The objective of this study is to understand the adaptation of an Arctic diatom to its environment by studying its ability to a...
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Online Access: | https://hal.science/hal-04203878 https://doi.org/10.1371/journal.pone.0272822 |
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ftccsdartic:oai:HAL:hal-04203878v1 2023-10-09T21:49:07+02:00 Photoacclimation of the polar diatom Chaetoceros neogracilis at low temperature Lacour, Thomas Larivière, Jade Ferland, Joannie Morin, Philippe-Israël Grondin, Pierre-Luc Donaher, Natalie Cockshutt, Amanda Campbell, Douglas A. Babin, Marcel Physiologie et Toxines des Microalgues Toxiques et Nuisibles (PHYTOX) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2022-09 https://hal.science/hal-04203878 https://doi.org/10.1371/journal.pone.0272822 en eng HAL CCSD Public Library of Science info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0272822 hal-04203878 https://hal.science/hal-04203878 doi:10.1371/journal.pone.0272822 ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://hal.science/hal-04203878 PLoS ONE, 2022, 17 (9), e0272822 (20p.). ⟨10.1371/journal.pone.0272822⟩ [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2022 ftccsdartic https://doi.org/10.1371/journal.pone.0272822 2023-09-23T22:52:41Z Polar microalgae face two major challenges: 1- growing at temperatures (-1.7 to 5°C) that limit enzyme kinetics; and 2- surviving and exploiting a wide range of irradiance. The objective of this study is to understand the adaptation of an Arctic diatom to its environment by studying its ability to acclimate to changes in light and temperature. We acclimated the polar diatom Chaetoceros neogracilis to various light levels at two different temperatures and studied its growth and photosynthetic properties using semi-continuous cultures. Rubisco content was high, to compensate for low catalytic rates, but did not change detectably with growth temperature. Contrary to what is observed in temperate species, in C. neogracilis, carbon fixation rate (20 min 14C incorporation) equaled net growth rate (μ) suggesting very low or very rapid (<20 min) re-oxidation of the newly fixed carbon. The comparison of saturation irradiances for electron transport, oxygen net production and carbon fixation revealed alternative electron pathways that could provide energy and reducing power to the cell without consuming organic carbon which is a very limiting product at low temperatures. High protein contents, low re-oxidation of newly fixed carbon and the use of electron pathways alternative to carbon fixation may be important characteristics allowing efficient growth under those extreme environmental conditions. Article in Journal/Newspaper Arctic Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Arctic PLOS ONE 17 9 e0272822 |
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Open Polar |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDV]Life Sciences [q-bio] |
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[SDV]Life Sciences [q-bio] Lacour, Thomas Larivière, Jade Ferland, Joannie Morin, Philippe-Israël Grondin, Pierre-Luc Donaher, Natalie Cockshutt, Amanda Campbell, Douglas A. Babin, Marcel Photoacclimation of the polar diatom Chaetoceros neogracilis at low temperature |
topic_facet |
[SDV]Life Sciences [q-bio] |
description |
Polar microalgae face two major challenges: 1- growing at temperatures (-1.7 to 5°C) that limit enzyme kinetics; and 2- surviving and exploiting a wide range of irradiance. The objective of this study is to understand the adaptation of an Arctic diatom to its environment by studying its ability to acclimate to changes in light and temperature. We acclimated the polar diatom Chaetoceros neogracilis to various light levels at two different temperatures and studied its growth and photosynthetic properties using semi-continuous cultures. Rubisco content was high, to compensate for low catalytic rates, but did not change detectably with growth temperature. Contrary to what is observed in temperate species, in C. neogracilis, carbon fixation rate (20 min 14C incorporation) equaled net growth rate (μ) suggesting very low or very rapid (<20 min) re-oxidation of the newly fixed carbon. The comparison of saturation irradiances for electron transport, oxygen net production and carbon fixation revealed alternative electron pathways that could provide energy and reducing power to the cell without consuming organic carbon which is a very limiting product at low temperatures. High protein contents, low re-oxidation of newly fixed carbon and the use of electron pathways alternative to carbon fixation may be important characteristics allowing efficient growth under those extreme environmental conditions. |
author2 |
Physiologie et Toxines des Microalgues Toxiques et Nuisibles (PHYTOX) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Lacour, Thomas Larivière, Jade Ferland, Joannie Morin, Philippe-Israël Grondin, Pierre-Luc Donaher, Natalie Cockshutt, Amanda Campbell, Douglas A. Babin, Marcel |
author_facet |
Lacour, Thomas Larivière, Jade Ferland, Joannie Morin, Philippe-Israël Grondin, Pierre-Luc Donaher, Natalie Cockshutt, Amanda Campbell, Douglas A. Babin, Marcel |
author_sort |
Lacour, Thomas |
title |
Photoacclimation of the polar diatom Chaetoceros neogracilis at low temperature |
title_short |
Photoacclimation of the polar diatom Chaetoceros neogracilis at low temperature |
title_full |
Photoacclimation of the polar diatom Chaetoceros neogracilis at low temperature |
title_fullStr |
Photoacclimation of the polar diatom Chaetoceros neogracilis at low temperature |
title_full_unstemmed |
Photoacclimation of the polar diatom Chaetoceros neogracilis at low temperature |
title_sort |
photoacclimation of the polar diatom chaetoceros neogracilis at low temperature |
publisher |
HAL CCSD |
publishDate |
2022 |
url |
https://hal.science/hal-04203878 https://doi.org/10.1371/journal.pone.0272822 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://hal.science/hal-04203878 PLoS ONE, 2022, 17 (9), e0272822 (20p.). ⟨10.1371/journal.pone.0272822⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0272822 hal-04203878 https://hal.science/hal-04203878 doi:10.1371/journal.pone.0272822 |
op_doi |
https://doi.org/10.1371/journal.pone.0272822 |
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PLOS ONE |
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17 |
container_issue |
9 |
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e0272822 |
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