First detection of OsHV-1 in the cephalopod Octopus vulgaris. Is the octopus a dead-end for OsHV-1?
The ostreid herpes virus (OsHV-1), associated with massive mortalities in the bivalve Crassostrea gigas, was detected for the first time in the cephalopod Octopus vulgaris. Wild adult animals from a natural breeding area in Spain showed an overall prevalence of detection of 87.5% between 2010 and 20...
Published in: | Journal of Invertebrate Pathology |
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Online Access: | https://hal.science/hal-04203340 https://doi.org/10.1016/j.jip.2021.107553 |
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ftccsdartic:oai:HAL:hal-04203340v1 2023-10-09T21:50:55+02:00 First detection of OsHV-1 in the cephalopod Octopus vulgaris. Is the octopus a dead-end for OsHV-1? Prado-Alvarez, Maria García-Fernández, Pablo Faury, Nicole Azevedo, Carlos Morga, Benjamin Gestal, Camino Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM) Santé, Génétique et Microbiologie des Mollusques (SGMM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) European Project: 659072,H2020,H2020-MSCA-IF-2014,ResisGal(2015) 2021-07 https://hal.science/hal-04203340 https://doi.org/10.1016/j.jip.2021.107553 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jip.2021.107553 info:eu-repo/grantAgreement//659072/EU/Ostreid herpesvirus 1: Genetic selection of Resistant strains and environmental interaction in the Atlantic coast of Spain (Galicia)/ResisGal hal-04203340 https://hal.science/hal-04203340 doi:10.1016/j.jip.2021.107553 ISSN: 0022-2011 EISSN: 1096-0805 Journal of Invertebrate Pathology https://hal.science/hal-04203340 Journal of Invertebrate Pathology, 2021, 183, 107553 (13p.). ⟨10.1016/j.jip.2021.107553⟩ [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2021 ftccsdartic https://doi.org/10.1016/j.jip.2021.107553 2023-09-23T22:53:22Z The ostreid herpes virus (OsHV-1), associated with massive mortalities in the bivalve Crassostrea gigas, was detected for the first time in the cephalopod Octopus vulgaris. Wild adult animals from a natural breeding area in Spain showed an overall prevalence of detection of 87.5% between 2010 and 2015 suggesting an environmental source of viral material uptake. Overall positive PCR detections were significantly higher in adult animals (p = 0.031) compared to newly hatched paralarvae (62%). Prevalence in embryos reached 65%. Sequencing of positive amplicons revealed a match with the variant OsHV-1 µVar showing the genomic features that distinguish this variant in the ORF4. Gill tissues from adult animals were also processed for in situ hybridization and revealed positive labelling. Experimental exposure trials in octopus paralarvae were carried out by cohabitation with virus injected oysters and by immersion in viral suspension observing a significant decrease in paralarval survival in both experiments. An increase in the number of OsHV-1 positive animals was detected in dead paralarvae after cohabitation with virus injected oysters. No signs of viral replication were observed based on lack of viral gene expression or visualization of viral structures by transmission electron microscopy. The octopus response against OsHV-1 was evaluated by gene expression of previously reported transcripts involved in immune response in C. gigas suggesting that immune defences in octopus are also activated after exposure to OsHV-1. Article in Journal/Newspaper Crassostrea gigas Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Journal of Invertebrate Pathology 183 107553 |
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Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDV]Life Sciences [q-bio] |
spellingShingle |
[SDV]Life Sciences [q-bio] Prado-Alvarez, Maria García-Fernández, Pablo Faury, Nicole Azevedo, Carlos Morga, Benjamin Gestal, Camino First detection of OsHV-1 in the cephalopod Octopus vulgaris. Is the octopus a dead-end for OsHV-1? |
topic_facet |
[SDV]Life Sciences [q-bio] |
description |
The ostreid herpes virus (OsHV-1), associated with massive mortalities in the bivalve Crassostrea gigas, was detected for the first time in the cephalopod Octopus vulgaris. Wild adult animals from a natural breeding area in Spain showed an overall prevalence of detection of 87.5% between 2010 and 2015 suggesting an environmental source of viral material uptake. Overall positive PCR detections were significantly higher in adult animals (p = 0.031) compared to newly hatched paralarvae (62%). Prevalence in embryos reached 65%. Sequencing of positive amplicons revealed a match with the variant OsHV-1 µVar showing the genomic features that distinguish this variant in the ORF4. Gill tissues from adult animals were also processed for in situ hybridization and revealed positive labelling. Experimental exposure trials in octopus paralarvae were carried out by cohabitation with virus injected oysters and by immersion in viral suspension observing a significant decrease in paralarval survival in both experiments. An increase in the number of OsHV-1 positive animals was detected in dead paralarvae after cohabitation with virus injected oysters. No signs of viral replication were observed based on lack of viral gene expression or visualization of viral structures by transmission electron microscopy. The octopus response against OsHV-1 was evaluated by gene expression of previously reported transcripts involved in immune response in C. gigas suggesting that immune defences in octopus are also activated after exposure to OsHV-1. |
author2 |
Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM) Santé, Génétique et Microbiologie des Mollusques (SGMM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) European Project: 659072,H2020,H2020-MSCA-IF-2014,ResisGal(2015) |
format |
Article in Journal/Newspaper |
author |
Prado-Alvarez, Maria García-Fernández, Pablo Faury, Nicole Azevedo, Carlos Morga, Benjamin Gestal, Camino |
author_facet |
Prado-Alvarez, Maria García-Fernández, Pablo Faury, Nicole Azevedo, Carlos Morga, Benjamin Gestal, Camino |
author_sort |
Prado-Alvarez, Maria |
title |
First detection of OsHV-1 in the cephalopod Octopus vulgaris. Is the octopus a dead-end for OsHV-1? |
title_short |
First detection of OsHV-1 in the cephalopod Octopus vulgaris. Is the octopus a dead-end for OsHV-1? |
title_full |
First detection of OsHV-1 in the cephalopod Octopus vulgaris. Is the octopus a dead-end for OsHV-1? |
title_fullStr |
First detection of OsHV-1 in the cephalopod Octopus vulgaris. Is the octopus a dead-end for OsHV-1? |
title_full_unstemmed |
First detection of OsHV-1 in the cephalopod Octopus vulgaris. Is the octopus a dead-end for OsHV-1? |
title_sort |
first detection of oshv-1 in the cephalopod octopus vulgaris. is the octopus a dead-end for oshv-1? |
publisher |
HAL CCSD |
publishDate |
2021 |
url |
https://hal.science/hal-04203340 https://doi.org/10.1016/j.jip.2021.107553 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0022-2011 EISSN: 1096-0805 Journal of Invertebrate Pathology https://hal.science/hal-04203340 Journal of Invertebrate Pathology, 2021, 183, 107553 (13p.). ⟨10.1016/j.jip.2021.107553⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jip.2021.107553 info:eu-repo/grantAgreement//659072/EU/Ostreid herpesvirus 1: Genetic selection of Resistant strains and environmental interaction in the Atlantic coast of Spain (Galicia)/ResisGal hal-04203340 https://hal.science/hal-04203340 doi:10.1016/j.jip.2021.107553 |
op_doi |
https://doi.org/10.1016/j.jip.2021.107553 |
container_title |
Journal of Invertebrate Pathology |
container_volume |
183 |
container_start_page |
107553 |
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1779314012334325760 |