First detection of Ostreid herpesvirus 1 in wild Crassostrea gigas in Argentina
Ostreid herpesvirus 1 (OsHV-1) is a DNA virus of the genus Ostreavirus (Malacoherpesviridae family, Herpesvirales order). Worldwide, OsHV-1 and its microvariants have been associated with increased mortality of Pacific oysters, Crassostrea gigas. Adult asymptomatic oysters also have shown a high pre...
Published in: | Journal of Invertebrate Pathology |
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Online Access: | https://hal.science/hal-04202350 https://doi.org/10.1016/j.jip.2019.107222 |
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ftccsdartic:oai:HAL:hal-04202350v1 2023-10-09T21:50:51+02:00 First detection of Ostreid herpesvirus 1 in wild Crassostrea gigas in Argentina Barbieri, Elena S. Medina, Cintia D. Vázquez, Nuria Fiorito, Carla Martelli, Antonela Wigdorovitz, Andrés Schwindt, Evangelina Morga, Benjamin Renault, Tristan Parreño, Viviana Barón, Pedro J Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM) Santé, Génétique et Microbiologie des Mollusques (SGMM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2019-09 https://hal.science/hal-04202350 https://doi.org/10.1016/j.jip.2019.107222 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jip.2019.107222 hal-04202350 https://hal.science/hal-04202350 doi:10.1016/j.jip.2019.107222 ISSN: 0022-2011 EISSN: 1096-0805 Journal of Invertebrate Pathology https://hal.science/hal-04202350 Journal of Invertebrate Pathology, 2019, 166, 107222 (6p.). ⟨10.1016/j.jip.2019.107222⟩ [SDV]Life Sciences [q-bio] [SDU]Sciences of the Universe [physics] info:eu-repo/semantics/article Journal articles 2019 ftccsdartic https://doi.org/10.1016/j.jip.2019.107222 2023-09-23T22:54:34Z Ostreid herpesvirus 1 (OsHV-1) is a DNA virus of the genus Ostreavirus (Malacoherpesviridae family, Herpesvirales order). Worldwide, OsHV-1 and its microvariants have been associated with increased mortality of Pacific oysters, Crassostrea gigas. Adult asymptomatic oysters also have shown a high prevalence of viral infection. As a consequence, surveillance is needed to better describe OsHV-1 diversity, pathogenicity, clinical signs, and geographical distribution. We examined Crassostrea gigas sampled in October 2017 from the inner zone of the Bahía Blanca Estuary, Argentina, and found that 8 of 30 specimens (26.7%) presented macroscopic lesions in mantle tissues. Histological analysis revealed abnormal presentation of mantle epithelial cells and connective tissues. Conventional and real-time PCR conducted on the oyster samples revealed 70% to be positive for presence of OsHV-1 DNA. The nucleotide sequence of the amplicon obtained from one sample using the primer pair IA1/IA2 (targeting ORF 42/43) was 99% identical to OsHV-1 reference as well as µVar strains B and A (KY271630, KY242785.1), sequenced from France and Ireland. This finding represents the first detection of OsHV-1 DNA in a wild population of C. gigas in Argentina in association with gross mantle lesions. Article in Journal/Newspaper Crassostrea gigas Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Argentina Journal of Invertebrate Pathology 166 107222 |
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Open Polar |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDV]Life Sciences [q-bio] [SDU]Sciences of the Universe [physics] |
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[SDV]Life Sciences [q-bio] [SDU]Sciences of the Universe [physics] Barbieri, Elena S. Medina, Cintia D. Vázquez, Nuria Fiorito, Carla Martelli, Antonela Wigdorovitz, Andrés Schwindt, Evangelina Morga, Benjamin Renault, Tristan Parreño, Viviana Barón, Pedro J First detection of Ostreid herpesvirus 1 in wild Crassostrea gigas in Argentina |
topic_facet |
[SDV]Life Sciences [q-bio] [SDU]Sciences of the Universe [physics] |
description |
Ostreid herpesvirus 1 (OsHV-1) is a DNA virus of the genus Ostreavirus (Malacoherpesviridae family, Herpesvirales order). Worldwide, OsHV-1 and its microvariants have been associated with increased mortality of Pacific oysters, Crassostrea gigas. Adult asymptomatic oysters also have shown a high prevalence of viral infection. As a consequence, surveillance is needed to better describe OsHV-1 diversity, pathogenicity, clinical signs, and geographical distribution. We examined Crassostrea gigas sampled in October 2017 from the inner zone of the Bahía Blanca Estuary, Argentina, and found that 8 of 30 specimens (26.7%) presented macroscopic lesions in mantle tissues. Histological analysis revealed abnormal presentation of mantle epithelial cells and connective tissues. Conventional and real-time PCR conducted on the oyster samples revealed 70% to be positive for presence of OsHV-1 DNA. The nucleotide sequence of the amplicon obtained from one sample using the primer pair IA1/IA2 (targeting ORF 42/43) was 99% identical to OsHV-1 reference as well as µVar strains B and A (KY271630, KY242785.1), sequenced from France and Ireland. This finding represents the first detection of OsHV-1 DNA in a wild population of C. gigas in Argentina in association with gross mantle lesions. |
author2 |
Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM) Santé, Génétique et Microbiologie des Mollusques (SGMM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Barbieri, Elena S. Medina, Cintia D. Vázquez, Nuria Fiorito, Carla Martelli, Antonela Wigdorovitz, Andrés Schwindt, Evangelina Morga, Benjamin Renault, Tristan Parreño, Viviana Barón, Pedro J |
author_facet |
Barbieri, Elena S. Medina, Cintia D. Vázquez, Nuria Fiorito, Carla Martelli, Antonela Wigdorovitz, Andrés Schwindt, Evangelina Morga, Benjamin Renault, Tristan Parreño, Viviana Barón, Pedro J |
author_sort |
Barbieri, Elena S. |
title |
First detection of Ostreid herpesvirus 1 in wild Crassostrea gigas in Argentina |
title_short |
First detection of Ostreid herpesvirus 1 in wild Crassostrea gigas in Argentina |
title_full |
First detection of Ostreid herpesvirus 1 in wild Crassostrea gigas in Argentina |
title_fullStr |
First detection of Ostreid herpesvirus 1 in wild Crassostrea gigas in Argentina |
title_full_unstemmed |
First detection of Ostreid herpesvirus 1 in wild Crassostrea gigas in Argentina |
title_sort |
first detection of ostreid herpesvirus 1 in wild crassostrea gigas in argentina |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.science/hal-04202350 https://doi.org/10.1016/j.jip.2019.107222 |
geographic |
Pacific Argentina |
geographic_facet |
Pacific Argentina |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0022-2011 EISSN: 1096-0805 Journal of Invertebrate Pathology https://hal.science/hal-04202350 Journal of Invertebrate Pathology, 2019, 166, 107222 (6p.). ⟨10.1016/j.jip.2019.107222⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jip.2019.107222 hal-04202350 https://hal.science/hal-04202350 doi:10.1016/j.jip.2019.107222 |
op_doi |
https://doi.org/10.1016/j.jip.2019.107222 |
container_title |
Journal of Invertebrate Pathology |
container_volume |
166 |
container_start_page |
107222 |
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1779313923897425920 |