Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat
High mortality rates are reported in spat and larvae of Pacific oyster Crassostrea gigas and associated with ostreid herpesvirus 1 (OsHV-1) detection in France. Although the viral infection has been experimentally reproduced in oyster larvae and spat, little knowledge is currently available concerni...
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ftccsdartic:oai:HAL:hal-04200733v1 2023-10-09T21:50:54+02:00 Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat Segarra, Amelie Baillon, Laury Faury, Nicole Tourbiez, Delphine Renault, Tristan Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM) Santé, Génétique et Microbiologie des Mollusques (SGMM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) European Project: 266157,EC:FP7:KBBE,FP7-KBBE-2010-4,BIVALIFE(2011) 2016-01 https://hal.science/hal-04200733 https://doi.org/10.1016/j.jip.2015.11.013 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jip.2015.11.013 info:eu-repo/grantAgreement/EC/FP7/266157/EU/Controlling infectious diseases in oysters and mussels in Europe/BIVALIFE hal-04200733 https://hal.science/hal-04200733 doi:10.1016/j.jip.2015.11.013 ISSN: 0022-2011 EISSN: 1096-0805 Journal of Invertebrate Pathology https://hal.science/hal-04200733 Journal of Invertebrate Pathology, 2016, 133, pp.59-65. ⟨10.1016/j.jip.2015.11.013⟩ [SDV]Life Sciences [q-bio] [SDU]Sciences of the Universe [physics] info:eu-repo/semantics/article Journal articles 2016 ftccsdartic https://doi.org/10.1016/j.jip.2015.11.013 2023-09-23T22:56:37Z High mortality rates are reported in spat and larvae of Pacific oyster Crassostrea gigas and associated with ostreid herpesvirus 1 (OsHV-1) detection in France. Although the viral infection has been experimentally reproduced in oyster larvae and spat, little knowledge is currently available concerning the viral entry and its distribution in organs and tissues. This study compares OsHV-1 DNA and RNA detection and localization in experimentally infected oysters using two virus doses: a low dose that did not induce any mortality and a high dose inducing high mortality. Real time PCR demonstrated significant differences in terms of viral DNA amounts between the two virus doses. RNA transcripts were detected in oysters receiving the highest dose of viral suspension whereas no transcript was observed in oysters injected with the low dose. This study also allowed observing kinetics of viral DNA and RNA detection in different tissues of oyster spat. Finally, viral detection was significantly different in function of tissues (p < 0.005), time (p < 0.005) with an interaction between tissues and time (p < 0.005) for each probe. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Journal of Invertebrate Pathology 133 59 65 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDV]Life Sciences [q-bio] [SDU]Sciences of the Universe [physics] |
spellingShingle |
[SDV]Life Sciences [q-bio] [SDU]Sciences of the Universe [physics] Segarra, Amelie Baillon, Laury Faury, Nicole Tourbiez, Delphine Renault, Tristan Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat |
topic_facet |
[SDV]Life Sciences [q-bio] [SDU]Sciences of the Universe [physics] |
description |
High mortality rates are reported in spat and larvae of Pacific oyster Crassostrea gigas and associated with ostreid herpesvirus 1 (OsHV-1) detection in France. Although the viral infection has been experimentally reproduced in oyster larvae and spat, little knowledge is currently available concerning the viral entry and its distribution in organs and tissues. This study compares OsHV-1 DNA and RNA detection and localization in experimentally infected oysters using two virus doses: a low dose that did not induce any mortality and a high dose inducing high mortality. Real time PCR demonstrated significant differences in terms of viral DNA amounts between the two virus doses. RNA transcripts were detected in oysters receiving the highest dose of viral suspension whereas no transcript was observed in oysters injected with the low dose. This study also allowed observing kinetics of viral DNA and RNA detection in different tissues of oyster spat. Finally, viral detection was significantly different in function of tissues (p < 0.005), time (p < 0.005) with an interaction between tissues and time (p < 0.005) for each probe. |
author2 |
Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM) Santé, Génétique et Microbiologie des Mollusques (SGMM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) European Project: 266157,EC:FP7:KBBE,FP7-KBBE-2010-4,BIVALIFE(2011) |
format |
Article in Journal/Newspaper |
author |
Segarra, Amelie Baillon, Laury Faury, Nicole Tourbiez, Delphine Renault, Tristan |
author_facet |
Segarra, Amelie Baillon, Laury Faury, Nicole Tourbiez, Delphine Renault, Tristan |
author_sort |
Segarra, Amelie |
title |
Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat |
title_short |
Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat |
title_full |
Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat |
title_fullStr |
Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat |
title_full_unstemmed |
Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat |
title_sort |
detection and distribution of ostreid herpesvirus 1 in experimentally infected pacific oyster spat |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal.science/hal-04200733 https://doi.org/10.1016/j.jip.2015.11.013 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0022-2011 EISSN: 1096-0805 Journal of Invertebrate Pathology https://hal.science/hal-04200733 Journal of Invertebrate Pathology, 2016, 133, pp.59-65. ⟨10.1016/j.jip.2015.11.013⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jip.2015.11.013 info:eu-repo/grantAgreement/EC/FP7/266157/EU/Controlling infectious diseases in oysters and mussels in Europe/BIVALIFE hal-04200733 https://hal.science/hal-04200733 doi:10.1016/j.jip.2015.11.013 |
op_doi |
https://doi.org/10.1016/j.jip.2015.11.013 |
container_title |
Journal of Invertebrate Pathology |
container_volume |
133 |
container_start_page |
59 |
op_container_end_page |
65 |
_version_ |
1779313991883948032 |