Autotetraploid Pacific oysters (Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability
We describe two methods of producing viable and fertile autotetraploid Pacific oyster (Crassostrea gigas Thunberg) based on the use of normal-sized oocytes produced by normal diploid females. Our methods showed that the oocyte size is not a limiting factor for the success of the induction to autotet...
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Online Access: | https://hal.science/hal-04200621 https://doi.org/10.1139/gen-2015-0014 |
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ftccsdartic:oai:HAL:hal-04200621v1 2023-10-09T21:50:52+02:00 Autotetraploid Pacific oysters (Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability Benabdelmouna, Abdellah Ledu, Christophe Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM) Santé, Génétique et Microbiologie des Mollusques (SGMM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2015-07 https://hal.science/hal-04200621 https://doi.org/10.1139/gen-2015-0014 en eng HAL CCSD NRC Research Press info:eu-repo/semantics/altIdentifier/doi/10.1139/gen-2015-0014 hal-04200621 https://hal.science/hal-04200621 doi:10.1139/gen-2015-0014 ISSN: 0831-2796 EISSN: 1480-3321 Genome https://hal.science/hal-04200621 Genome, 2015, 58 (7), pp.333-348. ⟨10.1139/gen-2015-0014⟩ [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2015 ftccsdartic https://doi.org/10.1139/gen-2015-0014 2023-09-23T22:56:50Z We describe two methods of producing viable and fertile autotetraploid Pacific oyster (Crassostrea gigas Thunberg) based on the use of normal-sized oocytes produced by normal diploid females. Our methods showed that the oocyte size is not a limiting factor for the success of the induction to autotetraploidy. These methods offer means of direct introgression of genetic progress from elite diploid lines to tetraploids used as broodstock, avoiding atriploid step with the risk of transferring undesirable traits from highly fecund triploids. High variability in the level of cytogenetic stability was found among the different tetraploid oysters tested showing that induction method has an important impact on the long-term cytogenetic stability of the tetraploids. It appears that induction method based on the use of triploid females induces a greater cytogenetic instability among tetraploids so obtained, and this compared to tetraploids originating from the two methods described in our present study. As the aneuploidies and reversions observed in tetraploids can have serious consequences for the sustainability of tetraploid broodstock itself as well as their triploid offspring, the two tetraploid induction methods described in the present work offer means to produce tetraploids with optimal cytogenetic, genetic and zootechnical performances Nous décrivons deux nouvelles méthodes de production d’huîtres du Pacific (Crassostrea gigas Thuneberg) autotétraploïdes basées sur l’utilisation d’ovocytes de taille normale issus de femelles diploïdes normales montrant ainsi que la taille des ovocytes n’est pas un facteur limitant pour la réussite de l’induction à la tétraploïdie. Ces méthodes permettent l’amélioration génétique des souches tétraploïdes en y transférant le progrès génétique réalisédans le compartiment diploïde et ceci tout en évitant le risque de transférer les caractères indésirables potentiellement transmis par les femelles triploïdes hyper fécondes. Des différences très nettes concernant la stabilité ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Genome 58 7 333 348 |
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Open Polar |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDV]Life Sciences [q-bio] |
spellingShingle |
[SDV]Life Sciences [q-bio] Benabdelmouna, Abdellah Ledu, Christophe Autotetraploid Pacific oysters (Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability |
topic_facet |
[SDV]Life Sciences [q-bio] |
description |
We describe two methods of producing viable and fertile autotetraploid Pacific oyster (Crassostrea gigas Thunberg) based on the use of normal-sized oocytes produced by normal diploid females. Our methods showed that the oocyte size is not a limiting factor for the success of the induction to autotetraploidy. These methods offer means of direct introgression of genetic progress from elite diploid lines to tetraploids used as broodstock, avoiding atriploid step with the risk of transferring undesirable traits from highly fecund triploids. High variability in the level of cytogenetic stability was found among the different tetraploid oysters tested showing that induction method has an important impact on the long-term cytogenetic stability of the tetraploids. It appears that induction method based on the use of triploid females induces a greater cytogenetic instability among tetraploids so obtained, and this compared to tetraploids originating from the two methods described in our present study. As the aneuploidies and reversions observed in tetraploids can have serious consequences for the sustainability of tetraploid broodstock itself as well as their triploid offspring, the two tetraploid induction methods described in the present work offer means to produce tetraploids with optimal cytogenetic, genetic and zootechnical performances Nous décrivons deux nouvelles méthodes de production d’huîtres du Pacific (Crassostrea gigas Thuneberg) autotétraploïdes basées sur l’utilisation d’ovocytes de taille normale issus de femelles diploïdes normales montrant ainsi que la taille des ovocytes n’est pas un facteur limitant pour la réussite de l’induction à la tétraploïdie. Ces méthodes permettent l’amélioration génétique des souches tétraploïdes en y transférant le progrès génétique réalisédans le compartiment diploïde et ceci tout en évitant le risque de transférer les caractères indésirables potentiellement transmis par les femelles triploïdes hyper fécondes. Des différences très nettes concernant la stabilité ... |
author2 |
Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM) Santé, Génétique et Microbiologie des Mollusques (SGMM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Benabdelmouna, Abdellah Ledu, Christophe |
author_facet |
Benabdelmouna, Abdellah Ledu, Christophe |
author_sort |
Benabdelmouna, Abdellah |
title |
Autotetraploid Pacific oysters (Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability |
title_short |
Autotetraploid Pacific oysters (Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability |
title_full |
Autotetraploid Pacific oysters (Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability |
title_fullStr |
Autotetraploid Pacific oysters (Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability |
title_full_unstemmed |
Autotetraploid Pacific oysters (Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability |
title_sort |
autotetraploid pacific oysters (crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.science/hal-04200621 https://doi.org/10.1139/gen-2015-0014 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0831-2796 EISSN: 1480-3321 Genome https://hal.science/hal-04200621 Genome, 2015, 58 (7), pp.333-348. ⟨10.1139/gen-2015-0014⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1139/gen-2015-0014 hal-04200621 https://hal.science/hal-04200621 doi:10.1139/gen-2015-0014 |
op_doi |
https://doi.org/10.1139/gen-2015-0014 |
container_title |
Genome |
container_volume |
58 |
container_issue |
7 |
container_start_page |
333 |
op_container_end_page |
348 |
_version_ |
1779313935281815552 |