Development of a comet-FISH assay for the detection of DNA damage in hemocytes of Crassostrea gigas
In this work, the DNA-damaging effect of hydrogen peroxide on the structural integrity of nucleolar organizer regions (NORs) was studied for the first time by comet-FISH in the Pacific oyster Crassostrea gigas. Global DNA damage was assessed in hemocytes using an alkaline version of the comet assay....
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ftccsdartic:oai:HAL:hal-04200561v1 2023-10-09T21:50:52+02:00 Development of a comet-FISH assay for the detection of DNA damage in hemocytes of Crassostrea gigas Perez Garcia, Maria Concepcion Rouxel, Julien Akcha, Farida Laboratoire d'Écotoxicologie (LEX) Biogéochimie et Ecotoxicologie (BE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2015-04 https://hal.science/hal-04200561 https://doi.org/10.1016/j.aquatox.2015.01.022 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2015.01.022 hal-04200561 https://hal.science/hal-04200561 doi:10.1016/j.aquatox.2015.01.022 ISSN: 0166-445X Aquatic Toxicology https://hal.science/hal-04200561 Aquatic Toxicology, 2015, 161, pp.189-195. ⟨10.1016/j.aquatox.2015.01.022⟩ [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2015 ftccsdartic https://doi.org/10.1016/j.aquatox.2015.01.022 2023-09-23T22:57:02Z In this work, the DNA-damaging effect of hydrogen peroxide on the structural integrity of nucleolar organizer regions (NORs) was studied for the first time by comet-FISH in the Pacific oyster Crassostrea gigas. Global DNA damage was assessed in hemocytes using an alkaline version of the comet assay. Next, NOR sensitivity was analysed by mapping major rDNA repeat unit by fluorescence in situ hybridisation (FISH) on the same comet slides. Exposure of hemocytes to 100 μM of hydrogen peroxide induced a significant increase in both DNA damage and number of FISH-signals of major ribosomal genes versus the control. Moreover, a significant positive correlation was shown between DNA damage as measured by the comet assay (percentage of DNA in comet tail) and the number of signals present in comet tails. This study demonstrates the potential value of the comet-FISH assay for the study of DNA damage induced by genotoxicant exposure of target genes. It offers a perspective for better understanding the impact of genotoxicity on animal physiology and fitness. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pacific Aquatic Toxicology 161 189 195 |
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Open Polar |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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ftccsdartic |
language |
English |
topic |
[SDV]Life Sciences [q-bio] |
spellingShingle |
[SDV]Life Sciences [q-bio] Perez Garcia, Maria Concepcion Rouxel, Julien Akcha, Farida Development of a comet-FISH assay for the detection of DNA damage in hemocytes of Crassostrea gigas |
topic_facet |
[SDV]Life Sciences [q-bio] |
description |
In this work, the DNA-damaging effect of hydrogen peroxide on the structural integrity of nucleolar organizer regions (NORs) was studied for the first time by comet-FISH in the Pacific oyster Crassostrea gigas. Global DNA damage was assessed in hemocytes using an alkaline version of the comet assay. Next, NOR sensitivity was analysed by mapping major rDNA repeat unit by fluorescence in situ hybridisation (FISH) on the same comet slides. Exposure of hemocytes to 100 μM of hydrogen peroxide induced a significant increase in both DNA damage and number of FISH-signals of major ribosomal genes versus the control. Moreover, a significant positive correlation was shown between DNA damage as measured by the comet assay (percentage of DNA in comet tail) and the number of signals present in comet tails. This study demonstrates the potential value of the comet-FISH assay for the study of DNA damage induced by genotoxicant exposure of target genes. It offers a perspective for better understanding the impact of genotoxicity on animal physiology and fitness. |
author2 |
Laboratoire d'Écotoxicologie (LEX) Biogéochimie et Ecotoxicologie (BE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Perez Garcia, Maria Concepcion Rouxel, Julien Akcha, Farida |
author_facet |
Perez Garcia, Maria Concepcion Rouxel, Julien Akcha, Farida |
author_sort |
Perez Garcia, Maria Concepcion |
title |
Development of a comet-FISH assay for the detection of DNA damage in hemocytes of Crassostrea gigas |
title_short |
Development of a comet-FISH assay for the detection of DNA damage in hemocytes of Crassostrea gigas |
title_full |
Development of a comet-FISH assay for the detection of DNA damage in hemocytes of Crassostrea gigas |
title_fullStr |
Development of a comet-FISH assay for the detection of DNA damage in hemocytes of Crassostrea gigas |
title_full_unstemmed |
Development of a comet-FISH assay for the detection of DNA damage in hemocytes of Crassostrea gigas |
title_sort |
development of a comet-fish assay for the detection of dna damage in hemocytes of crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.science/hal-04200561 https://doi.org/10.1016/j.aquatox.2015.01.022 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0166-445X Aquatic Toxicology https://hal.science/hal-04200561 Aquatic Toxicology, 2015, 161, pp.189-195. ⟨10.1016/j.aquatox.2015.01.022⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2015.01.022 hal-04200561 https://hal.science/hal-04200561 doi:10.1016/j.aquatox.2015.01.022 |
op_doi |
https://doi.org/10.1016/j.aquatox.2015.01.022 |
container_title |
Aquatic Toxicology |
container_volume |
161 |
container_start_page |
189 |
op_container_end_page |
195 |
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1779313940044447744 |