High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas
International audience We report here the first evidence in an invertebrate, the oyster Crassostrea gigas, of a phenomenon of Presence-Absence Variation (PAV) affecting immune-related genes. We previously evidenced an extraordinary interindividual variability in the basal mRNA abundances of oyster i...
Published in: | Developmental & Comparative Immunology |
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Online Access: | https://hal.science/hal-04200548 https://hal.science/hal-04200548/document https://hal.science/hal-04200548/file/33545.pdf https://doi.org/10.1016/j.dci.2014.12.002 |
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ftccsdartic:oai:HAL:hal-04200548v1 2024-02-27T08:39:53+00:00 High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas Rosa, Rafael D. Alonso, Pascal Santini, Adrien Vergnes, Agnes Bachere, Evelyne Biologie des Organismes Marins Exploités (BOME) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) 2015-04 https://hal.science/hal-04200548 https://hal.science/hal-04200548/document https://hal.science/hal-04200548/file/33545.pdf https://doi.org/10.1016/j.dci.2014.12.002 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.dci.2014.12.002 hal-04200548 https://hal.science/hal-04200548 https://hal.science/hal-04200548/document https://hal.science/hal-04200548/file/33545.pdf doi:10.1016/j.dci.2014.12.002 info:eu-repo/semantics/OpenAccess ISSN: 0145-305X Developmental and Comparative Immunology https://hal.science/hal-04200548 Developmental and Comparative Immunology, 2015, 49 (2), pp.231-238. ⟨10.1016/j.dci.2014.12.002⟩ [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2015 ftccsdartic https://doi.org/10.1016/j.dci.2014.12.002 2024-01-28T00:30:38Z International audience We report here the first evidence in an invertebrate, the oyster Crassostrea gigas, of a phenomenon of Presence-Absence Variation (PAV) affecting immune-related genes. We previously evidenced an extraordinary interindividual variability in the basal mRNA abundances of oyster immune genes including those coding for a family of antimicrobial peptides, the big defensins (Cg-BigDef). Cg-BigDef is a diverse family composed of three members: Cg-BigDef1 to -3. Here, we show that besides a high polymorphism in Cg-BigDef mRNA expression, not all individual oysters express simultaneously the three Cg-BigDefs. Moreover, in numerous individuals, no expression of Cg-BigDefs could be detected. Further investigation at the genomic level revealed that in individuals in which the transcription of one or all Cg-BigDefs was absent the corresponding Cg-bigdef gene was missing. In our experiments, no correlation was found between Cg-bigdef PAV and oyster capacity to survive Vibrio infections. The discovery of P-A immune genes in oysters leads to reconsider the role that plays the immune system in the individual adaptation to survive environmental, biotic and abiotic stresses. Article in Journal/Newspaper Crassostrea gigas Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Developmental & Comparative Immunology 49 2 231 238 |
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Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
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English |
topic |
[SDV]Life Sciences [q-bio] |
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[SDV]Life Sciences [q-bio] Rosa, Rafael D. Alonso, Pascal Santini, Adrien Vergnes, Agnes Bachere, Evelyne High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas |
topic_facet |
[SDV]Life Sciences [q-bio] |
description |
International audience We report here the first evidence in an invertebrate, the oyster Crassostrea gigas, of a phenomenon of Presence-Absence Variation (PAV) affecting immune-related genes. We previously evidenced an extraordinary interindividual variability in the basal mRNA abundances of oyster immune genes including those coding for a family of antimicrobial peptides, the big defensins (Cg-BigDef). Cg-BigDef is a diverse family composed of three members: Cg-BigDef1 to -3. Here, we show that besides a high polymorphism in Cg-BigDef mRNA expression, not all individual oysters express simultaneously the three Cg-BigDefs. Moreover, in numerous individuals, no expression of Cg-BigDefs could be detected. Further investigation at the genomic level revealed that in individuals in which the transcription of one or all Cg-BigDefs was absent the corresponding Cg-bigdef gene was missing. In our experiments, no correlation was found between Cg-bigdef PAV and oyster capacity to survive Vibrio infections. The discovery of P-A immune genes in oysters leads to reconsider the role that plays the immune system in the individual adaptation to survive environmental, biotic and abiotic stresses. |
author2 |
Biologie des Organismes Marins Exploités (BOME) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) |
format |
Article in Journal/Newspaper |
author |
Rosa, Rafael D. Alonso, Pascal Santini, Adrien Vergnes, Agnes Bachere, Evelyne |
author_facet |
Rosa, Rafael D. Alonso, Pascal Santini, Adrien Vergnes, Agnes Bachere, Evelyne |
author_sort |
Rosa, Rafael D. |
title |
High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas |
title_short |
High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas |
title_full |
High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas |
title_fullStr |
High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas |
title_full_unstemmed |
High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas |
title_sort |
high polymorphism in big defensin gene expression reveals presence-absence gene variability (pav) in the oyster crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.science/hal-04200548 https://hal.science/hal-04200548/document https://hal.science/hal-04200548/file/33545.pdf https://doi.org/10.1016/j.dci.2014.12.002 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0145-305X Developmental and Comparative Immunology https://hal.science/hal-04200548 Developmental and Comparative Immunology, 2015, 49 (2), pp.231-238. ⟨10.1016/j.dci.2014.12.002⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.dci.2014.12.002 hal-04200548 https://hal.science/hal-04200548 https://hal.science/hal-04200548/document https://hal.science/hal-04200548/file/33545.pdf doi:10.1016/j.dci.2014.12.002 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.dci.2014.12.002 |
container_title |
Developmental & Comparative Immunology |
container_volume |
49 |
container_issue |
2 |
container_start_page |
231 |
op_container_end_page |
238 |
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1792046945550729216 |