Reversibility controls on extreme methane clumped isotope signatures from anaerobic oxidation of methane
International audience Microbial anaerobic oxidation of methane (AOM) substantially mitigates atmospheric methane emissions on Earth and is a process to consider for astrobiological targets where methane has been detected. The measurement of doubly substituted, or “clumped”, methane isotopes has pro...
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ftccsdartic:oai:HAL:hal-04021872v1 2023-12-24T10:25:14+01:00 Reversibility controls on extreme methane clumped isotope signatures from anaerobic oxidation of methane Liu, Jiarui Harris, Rachel Ash, Jeanine Ferry, James Krause, Sebastian J.E. Labidi, Jabrane Prakash, Divya Sherwood Lollar, Barbara Treude, Tina Warr, Oliver Young, Edward Department of Earth, Planetary and Space Sciences Los Angeles (EPSS) University of California Los Angeles (UCLA) University of California (UC)-University of California (UC) Department of Organismic and Evolutionary Biology Cambridge (OEB) Harvard University Earth, Planetary and Space Sciences, University of California, Los Angeles, CA, USA Pennsylvania State University (Penn State) Penn State System Institut de Physique du Globe de Paris (IPGP (UMR_7154)) Institut national des sciences de l'Univers (INSU - CNRS)-Université de La Réunion (UR)-Institut de Physique du Globe de Paris (IPG Paris)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité) Thapar Institute of Engineering and Technology Patiala, Inde (TIET) University of Toronto 2023 https://hal.science/hal-04021872 https://hal.science/hal-04021872/document https://hal.science/hal-04021872/file/GCA-D-22-00625_R1.pdf en eng HAL CCSD Elsevier hal-04021872 https://hal.science/hal-04021872 https://hal.science/hal-04021872/document https://hal.science/hal-04021872/file/GCA-D-22-00625_R1.pdf info:eu-repo/semantics/OpenAccess ISSN: 0016-7037 EISSN: 0016-7037 Geochimica et Cosmochimica Acta https://hal.science/hal-04021872 Geochimica et Cosmochimica Acta, In press methane isotopologues methyl-coenzyme M reductase kinetic fractionation equilibrium fractionation isotopic bond re-ordering [SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry info:eu-repo/semantics/article Journal articles 2023 ftccsdartic 2023-11-25T23:50:32Z International audience Microbial anaerobic oxidation of methane (AOM) substantially mitigates atmospheric methane emissions on Earth and is a process to consider for astrobiological targets where methane has been detected. The measurement of doubly substituted, or “clumped”, methane isotopes has proven useful in tracing processes of methane formation and oxidation. Both near-equilibrium and extreme disequilibrium methane clumped isotope signatures can be attributed to AOM, but, to date, understanding the mechanistic and environmental controls on those signatures have been lacking. We report measurements of methane clumped isotope compositions of residual methane in AOM-active microbial incubations using sediment slurries from Svalbard and Santa Barbara Channel methane seeps. Incubation experiments of Svalbard sediment slurries resulted in residual methane with very high Δ13CH3D and Δ12CH2D2 values up to 19.5‰ and 65.1‰, respectively. We found similarly high Δ13CH3D and Δ12CH2D2 values in fluid samples from the Chamorro Seamount, a serpentinite mud volcano in the Mariana forearc, suggesting that minimal reversibility of AOM intracellular reactions leads to kinetic fractionation of clumped isotopologues. When conditions were consistent with a low thermodynamic drive for AOM, however, methane isotopologues approached intra-species quasi-equilibrium. This was clearly observed in isotope exchange experiments with methyl-coenzyme M reductase (Mcr) and in microbial incubations of the Santa Barbara Channel sediment slurries. Using an isotopologue fractionation model, we highlight the critical role of reversibility in controlling the trajectory of gases in Δ13CH3D vs. Δ12CH2D2 space during AOM. The near-equilibrium methane isotopologue signatures are generalized as a result of the Mcr-catalyzed intracellular isotope exchange operating under near threshold free energy conditions, as shown in the deep-biosphere incubations. Our results show that the reversibility of the Mcr-catalyzed reaction is central to understanding ... Article in Journal/Newspaper Svalbard Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Svalbard |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
methane isotopologues methyl-coenzyme M reductase kinetic fractionation equilibrium fractionation isotopic bond re-ordering [SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry |
spellingShingle |
methane isotopologues methyl-coenzyme M reductase kinetic fractionation equilibrium fractionation isotopic bond re-ordering [SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry Liu, Jiarui Harris, Rachel Ash, Jeanine Ferry, James Krause, Sebastian J.E. Labidi, Jabrane Prakash, Divya Sherwood Lollar, Barbara Treude, Tina Warr, Oliver Young, Edward Reversibility controls on extreme methane clumped isotope signatures from anaerobic oxidation of methane |
topic_facet |
methane isotopologues methyl-coenzyme M reductase kinetic fractionation equilibrium fractionation isotopic bond re-ordering [SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry |
description |
International audience Microbial anaerobic oxidation of methane (AOM) substantially mitigates atmospheric methane emissions on Earth and is a process to consider for astrobiological targets where methane has been detected. The measurement of doubly substituted, or “clumped”, methane isotopes has proven useful in tracing processes of methane formation and oxidation. Both near-equilibrium and extreme disequilibrium methane clumped isotope signatures can be attributed to AOM, but, to date, understanding the mechanistic and environmental controls on those signatures have been lacking. We report measurements of methane clumped isotope compositions of residual methane in AOM-active microbial incubations using sediment slurries from Svalbard and Santa Barbara Channel methane seeps. Incubation experiments of Svalbard sediment slurries resulted in residual methane with very high Δ13CH3D and Δ12CH2D2 values up to 19.5‰ and 65.1‰, respectively. We found similarly high Δ13CH3D and Δ12CH2D2 values in fluid samples from the Chamorro Seamount, a serpentinite mud volcano in the Mariana forearc, suggesting that minimal reversibility of AOM intracellular reactions leads to kinetic fractionation of clumped isotopologues. When conditions were consistent with a low thermodynamic drive for AOM, however, methane isotopologues approached intra-species quasi-equilibrium. This was clearly observed in isotope exchange experiments with methyl-coenzyme M reductase (Mcr) and in microbial incubations of the Santa Barbara Channel sediment slurries. Using an isotopologue fractionation model, we highlight the critical role of reversibility in controlling the trajectory of gases in Δ13CH3D vs. Δ12CH2D2 space during AOM. The near-equilibrium methane isotopologue signatures are generalized as a result of the Mcr-catalyzed intracellular isotope exchange operating under near threshold free energy conditions, as shown in the deep-biosphere incubations. Our results show that the reversibility of the Mcr-catalyzed reaction is central to understanding ... |
author2 |
Department of Earth, Planetary and Space Sciences Los Angeles (EPSS) University of California Los Angeles (UCLA) University of California (UC)-University of California (UC) Department of Organismic and Evolutionary Biology Cambridge (OEB) Harvard University Earth, Planetary and Space Sciences, University of California, Los Angeles, CA, USA Pennsylvania State University (Penn State) Penn State System Institut de Physique du Globe de Paris (IPGP (UMR_7154)) Institut national des sciences de l'Univers (INSU - CNRS)-Université de La Réunion (UR)-Institut de Physique du Globe de Paris (IPG Paris)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité) Thapar Institute of Engineering and Technology Patiala, Inde (TIET) University of Toronto |
format |
Article in Journal/Newspaper |
author |
Liu, Jiarui Harris, Rachel Ash, Jeanine Ferry, James Krause, Sebastian J.E. Labidi, Jabrane Prakash, Divya Sherwood Lollar, Barbara Treude, Tina Warr, Oliver Young, Edward |
author_facet |
Liu, Jiarui Harris, Rachel Ash, Jeanine Ferry, James Krause, Sebastian J.E. Labidi, Jabrane Prakash, Divya Sherwood Lollar, Barbara Treude, Tina Warr, Oliver Young, Edward |
author_sort |
Liu, Jiarui |
title |
Reversibility controls on extreme methane clumped isotope signatures from anaerobic oxidation of methane |
title_short |
Reversibility controls on extreme methane clumped isotope signatures from anaerobic oxidation of methane |
title_full |
Reversibility controls on extreme methane clumped isotope signatures from anaerobic oxidation of methane |
title_fullStr |
Reversibility controls on extreme methane clumped isotope signatures from anaerobic oxidation of methane |
title_full_unstemmed |
Reversibility controls on extreme methane clumped isotope signatures from anaerobic oxidation of methane |
title_sort |
reversibility controls on extreme methane clumped isotope signatures from anaerobic oxidation of methane |
publisher |
HAL CCSD |
publishDate |
2023 |
url |
https://hal.science/hal-04021872 https://hal.science/hal-04021872/document https://hal.science/hal-04021872/file/GCA-D-22-00625_R1.pdf |
geographic |
Svalbard |
geographic_facet |
Svalbard |
genre |
Svalbard |
genre_facet |
Svalbard |
op_source |
ISSN: 0016-7037 EISSN: 0016-7037 Geochimica et Cosmochimica Acta https://hal.science/hal-04021872 Geochimica et Cosmochimica Acta, In press |
op_relation |
hal-04021872 https://hal.science/hal-04021872 https://hal.science/hal-04021872/document https://hal.science/hal-04021872/file/GCA-D-22-00625_R1.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1786200878111260672 |