Distribution of non-synonymous Vkorc1 mutations in roof rats (Rattus rattus) in France and in Spain - consequences for management
International audience Rodent control is mainly done using anticoagulant rodenticides leading to the death of rodents through internal bleeding by targeting the VKORC1 protein. However, mutations in VKORC1 can lead to resistance to anticoagulant rodenticides that can cause treatment failure in the f...
Published in: | Pesticide Biochemistry and Physiology |
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Format: | Article in Journal/Newspaper |
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Online Access: | https://hal.inrae.fr/hal-03882401 https://doi.org/10.1016/j.pestbp.2022.105052 |
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ftccsdartic:oai:HAL:hal-03882401v1 2023-12-31T10:22:22+01:00 Distribution of non-synonymous Vkorc1 mutations in roof rats (Rattus rattus) in France and in Spain - consequences for management Damin-Pernik, Marlène Hammed, Abdessalem Giraud, Ludivine Goulois, Joffrey Benoit, Etienne Lattard, Virginie Rongeurs Sauvages, Risques Sanitaires et Gestion des Populations - UR 1233 (RS2GP) VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Liphatech 2022-05 https://hal.inrae.fr/hal-03882401 https://doi.org/10.1016/j.pestbp.2022.105052 en eng HAL CCSD info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pestbp.2022.105052 info:eu-repo/semantics/altIdentifier/pmid/35430058 hal-03882401 https://hal.inrae.fr/hal-03882401 doi:10.1016/j.pestbp.2022.105052 PUBMED: 35430058 WOS: 000883372600001 http://creativecommons.org/licenses/by-nc-nd/ ISSN: 0048-3575 EISSN: 0048-3575 Pesticide Biochemistry and Physiology https://hal.inrae.fr/hal-03882401 Pesticide Biochemistry and Physiology, 2022, 183, pp.105052. ⟨10.1016/j.pestbp.2022.105052⟩ Rattus rattus Anticoagulant rodenticide VKORC1 Mutation Resistance VKOR activity assay [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology info:eu-repo/semantics/article Journal articles 2022 ftccsdartic https://doi.org/10.1016/j.pestbp.2022.105052 2023-12-02T23:46:51Z International audience Rodent control is mainly done using anticoagulant rodenticides leading to the death of rodents through internal bleeding by targeting the VKORC1 protein. However, mutations in VKORC1 can lead to resistance to anticoagulant rodenticides that can cause treatment failure in the field. This study provides the first insight into the distribution, frequency and characterization of Vkorc1 mutations in roof rats ( Rattus rattus) in France and in three administrative areas of Spain. The roof rat is present in France while it was thought to have almost disappeared with the expansion of the brown rat. Nevertheless, it has been found mainly in maritime areas. 151 roof rats out of 219 tested presented at least one missense mutation in the coding sequences of Vkorc1 gene (i.e. 69.0% of the rat). Nine Vkorc1 genotypes were detected (Y25F, A26P, R40G, S57F, W59C, W59R, H68N, Y25F/K152T and Y25F/W59R. Biochemical characterization of the consequences of these different genotypes proved that these various genotypes did not induce severe resistance to anticoagulant rodenticides. Even if many mutations of the Vkorc1 gene are present in roof rat populations in France, their management may be based in a first approach, considering the low levels of resistance induced, on the use of first-generation anticoagulants less dangerous for wildlife. The use of second-generation may be considered when treatment failure is observed or when bait consumption is limited. Article in Journal/Newspaper Rattus rattus Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Pesticide Biochemistry and Physiology 183 105052 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Rattus rattus Anticoagulant rodenticide VKORC1 Mutation Resistance VKOR activity assay [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology |
spellingShingle |
Rattus rattus Anticoagulant rodenticide VKORC1 Mutation Resistance VKOR activity assay [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Damin-Pernik, Marlène Hammed, Abdessalem Giraud, Ludivine Goulois, Joffrey Benoit, Etienne Lattard, Virginie Distribution of non-synonymous Vkorc1 mutations in roof rats (Rattus rattus) in France and in Spain - consequences for management |
topic_facet |
Rattus rattus Anticoagulant rodenticide VKORC1 Mutation Resistance VKOR activity assay [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology |
description |
International audience Rodent control is mainly done using anticoagulant rodenticides leading to the death of rodents through internal bleeding by targeting the VKORC1 protein. However, mutations in VKORC1 can lead to resistance to anticoagulant rodenticides that can cause treatment failure in the field. This study provides the first insight into the distribution, frequency and characterization of Vkorc1 mutations in roof rats ( Rattus rattus) in France and in three administrative areas of Spain. The roof rat is present in France while it was thought to have almost disappeared with the expansion of the brown rat. Nevertheless, it has been found mainly in maritime areas. 151 roof rats out of 219 tested presented at least one missense mutation in the coding sequences of Vkorc1 gene (i.e. 69.0% of the rat). Nine Vkorc1 genotypes were detected (Y25F, A26P, R40G, S57F, W59C, W59R, H68N, Y25F/K152T and Y25F/W59R. Biochemical characterization of the consequences of these different genotypes proved that these various genotypes did not induce severe resistance to anticoagulant rodenticides. Even if many mutations of the Vkorc1 gene are present in roof rat populations in France, their management may be based in a first approach, considering the low levels of resistance induced, on the use of first-generation anticoagulants less dangerous for wildlife. The use of second-generation may be considered when treatment failure is observed or when bait consumption is limited. |
author2 |
Rongeurs Sauvages, Risques Sanitaires et Gestion des Populations - UR 1233 (RS2GP) VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Liphatech |
format |
Article in Journal/Newspaper |
author |
Damin-Pernik, Marlène Hammed, Abdessalem Giraud, Ludivine Goulois, Joffrey Benoit, Etienne Lattard, Virginie |
author_facet |
Damin-Pernik, Marlène Hammed, Abdessalem Giraud, Ludivine Goulois, Joffrey Benoit, Etienne Lattard, Virginie |
author_sort |
Damin-Pernik, Marlène |
title |
Distribution of non-synonymous Vkorc1 mutations in roof rats (Rattus rattus) in France and in Spain - consequences for management |
title_short |
Distribution of non-synonymous Vkorc1 mutations in roof rats (Rattus rattus) in France and in Spain - consequences for management |
title_full |
Distribution of non-synonymous Vkorc1 mutations in roof rats (Rattus rattus) in France and in Spain - consequences for management |
title_fullStr |
Distribution of non-synonymous Vkorc1 mutations in roof rats (Rattus rattus) in France and in Spain - consequences for management |
title_full_unstemmed |
Distribution of non-synonymous Vkorc1 mutations in roof rats (Rattus rattus) in France and in Spain - consequences for management |
title_sort |
distribution of non-synonymous vkorc1 mutations in roof rats (rattus rattus) in france and in spain - consequences for management |
publisher |
HAL CCSD |
publishDate |
2022 |
url |
https://hal.inrae.fr/hal-03882401 https://doi.org/10.1016/j.pestbp.2022.105052 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
ISSN: 0048-3575 EISSN: 0048-3575 Pesticide Biochemistry and Physiology https://hal.inrae.fr/hal-03882401 Pesticide Biochemistry and Physiology, 2022, 183, pp.105052. ⟨10.1016/j.pestbp.2022.105052⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pestbp.2022.105052 info:eu-repo/semantics/altIdentifier/pmid/35430058 hal-03882401 https://hal.inrae.fr/hal-03882401 doi:10.1016/j.pestbp.2022.105052 PUBMED: 35430058 WOS: 000883372600001 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/ |
op_doi |
https://doi.org/10.1016/j.pestbp.2022.105052 |
container_title |
Pesticide Biochemistry and Physiology |
container_volume |
183 |
container_start_page |
105052 |
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