First Molecular Detection of Pathogens Leptospira in Common Rodent Captured in North Algeria Urban Areas
International audience Leptospirosis is an anthropozoonosis disease of worldwide distribution caused by mobile spirochetes of the genus Leptospira and rodents, mainly rats, are described as its primary reservoir. In Algeria, there is limited data about the prevalence of Leptospira spp. in humans and...
Published in: | Tropical Medicine and Infectious Disease |
---|---|
Main Authors: | , , , , , , , |
Other Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2022
|
Subjects: | |
Online Access: | https://hal.inrae.fr/hal-03882346 https://doi.org/10.3390/tropicalmed7110335 |
id |
ftccsdartic:oai:HAL:hal-03882346v1 |
---|---|
record_format |
openpolar |
spelling |
ftccsdartic:oai:HAL:hal-03882346v1 2024-02-11T10:08:13+01:00 First Molecular Detection of Pathogens Leptospira in Common Rodent Captured in North Algeria Urban Areas Lekhal, Lila Harran, Elena Aragon, Anaïs Groud, Karine Le Guyader, Marine Kaidi, Rachid Khelef, Djamel Djelouadji, Zouheira Université Saâd Dahlab Blida 1 (UB1) Rongeurs Sauvages, Risques Sanitaires et Gestion des Populations - UR 1233 (RS2GP) VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Université Saint-Esprit de Kaslik (USEK) Ecole Nationale Supérieure Vétérinaire d'Alger (ENSV) 2022-11 https://hal.inrae.fr/hal-03882346 https://doi.org/10.3390/tropicalmed7110335 en eng HAL CCSD MDPI info:eu-repo/semantics/altIdentifier/doi/10.3390/tropicalmed7110335 info:eu-repo/semantics/altIdentifier/pmid/36355878 hal-03882346 https://hal.inrae.fr/hal-03882346 doi:10.3390/tropicalmed7110335 PUBMED: 36355878 WOS: 000881601300001 http://creativecommons.org/licenses/by/ ISSN: 2414-6366 Tropical Medicine and Infectious Disease https://hal.inrae.fr/hal-03882346 Tropical Medicine and Infectious Disease, 2022, 7 (11), pp.335. ⟨10.3390/tropicalmed7110335⟩ leptospirosis Algeria Blida rodents L interrogans borpetersenii Rattus Norvegicus Rattus Rattus Mus Musculus [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases info:eu-repo/semantics/article Journal articles 2022 ftccsdartic https://doi.org/10.3390/tropicalmed7110335 2024-01-14T00:04:11Z International audience Leptospirosis is an anthropozoonosis disease of worldwide distribution caused by mobile spirochetes of the genus Leptospira and rodents, mainly rats, are described as its primary reservoir. In Algeria, there is limited data about the prevalence of Leptospira spp. in humans and animals, as well as Leptospira carriage in wild rodents. The study aimed to highlight the importance of rodents as a reservoir of Leptospira bacterium in Blida city in Algeria by detecting and identifying circulating Leptospira species in the rodent population. A total of 101 rodents, 95 Rattus Norvegicus, 5 Rattus Rattus, and 1 Mus Musculus were captured and tested for pathogenic Leptospira spp. byreal-time PCR targeting the Leptospira 16S rRNA (rrs) gene, revealing a total prevalence of 40.6%, 95% IC [30.9–50.8%]. Positive samples were subjected to species-specific real-time PCR assays targeting L. interrogans, L. noguchii, L. borgpetersenii, and L. kirschneri for species identification. However, positive samples for which Leptospira-species could not be determined were subjected to conventional PCR targeting the partial 16S rRNA (rrs) gene, and amplified DNA was subjected to sequencing. Leptospira spp. was detected in 36 kidney, 16 urine, and three lung specimens. L. interrogans was identified in 39 rodents and L. borpetersenii in one rodent; however, one rodent with renal carriage could not be typed due to poor DNA quality. This study provides the first description of pathogenic Leptospira spp. in wild rodents in Algeria. These findings suggest a high potential risk of leptospirosis transmission from rodents to humans and animals in Algeria and therefore imply the adoption of prophylactic measures. In addition, further studies, including different animals and rodent species, should be conducted to clarify the epidemiology of this disease in Algeria. Article in Journal/Newspaper Rattus rattus Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Tropical Medicine and Infectious Disease 7 11 335 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
leptospirosis Algeria Blida rodents L interrogans borpetersenii Rattus Norvegicus Rattus Rattus Mus Musculus [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases |
spellingShingle |
leptospirosis Algeria Blida rodents L interrogans borpetersenii Rattus Norvegicus Rattus Rattus Mus Musculus [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Lekhal, Lila Harran, Elena Aragon, Anaïs Groud, Karine Le Guyader, Marine Kaidi, Rachid Khelef, Djamel Djelouadji, Zouheira First Molecular Detection of Pathogens Leptospira in Common Rodent Captured in North Algeria Urban Areas |
topic_facet |
leptospirosis Algeria Blida rodents L interrogans borpetersenii Rattus Norvegicus Rattus Rattus Mus Musculus [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases |
description |
International audience Leptospirosis is an anthropozoonosis disease of worldwide distribution caused by mobile spirochetes of the genus Leptospira and rodents, mainly rats, are described as its primary reservoir. In Algeria, there is limited data about the prevalence of Leptospira spp. in humans and animals, as well as Leptospira carriage in wild rodents. The study aimed to highlight the importance of rodents as a reservoir of Leptospira bacterium in Blida city in Algeria by detecting and identifying circulating Leptospira species in the rodent population. A total of 101 rodents, 95 Rattus Norvegicus, 5 Rattus Rattus, and 1 Mus Musculus were captured and tested for pathogenic Leptospira spp. byreal-time PCR targeting the Leptospira 16S rRNA (rrs) gene, revealing a total prevalence of 40.6%, 95% IC [30.9–50.8%]. Positive samples were subjected to species-specific real-time PCR assays targeting L. interrogans, L. noguchii, L. borgpetersenii, and L. kirschneri for species identification. However, positive samples for which Leptospira-species could not be determined were subjected to conventional PCR targeting the partial 16S rRNA (rrs) gene, and amplified DNA was subjected to sequencing. Leptospira spp. was detected in 36 kidney, 16 urine, and three lung specimens. L. interrogans was identified in 39 rodents and L. borpetersenii in one rodent; however, one rodent with renal carriage could not be typed due to poor DNA quality. This study provides the first description of pathogenic Leptospira spp. in wild rodents in Algeria. These findings suggest a high potential risk of leptospirosis transmission from rodents to humans and animals in Algeria and therefore imply the adoption of prophylactic measures. In addition, further studies, including different animals and rodent species, should be conducted to clarify the epidemiology of this disease in Algeria. |
author2 |
Université Saâd Dahlab Blida 1 (UB1) Rongeurs Sauvages, Risques Sanitaires et Gestion des Populations - UR 1233 (RS2GP) VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Université Saint-Esprit de Kaslik (USEK) Ecole Nationale Supérieure Vétérinaire d'Alger (ENSV) |
format |
Article in Journal/Newspaper |
author |
Lekhal, Lila Harran, Elena Aragon, Anaïs Groud, Karine Le Guyader, Marine Kaidi, Rachid Khelef, Djamel Djelouadji, Zouheira |
author_facet |
Lekhal, Lila Harran, Elena Aragon, Anaïs Groud, Karine Le Guyader, Marine Kaidi, Rachid Khelef, Djamel Djelouadji, Zouheira |
author_sort |
Lekhal, Lila |
title |
First Molecular Detection of Pathogens Leptospira in Common Rodent Captured in North Algeria Urban Areas |
title_short |
First Molecular Detection of Pathogens Leptospira in Common Rodent Captured in North Algeria Urban Areas |
title_full |
First Molecular Detection of Pathogens Leptospira in Common Rodent Captured in North Algeria Urban Areas |
title_fullStr |
First Molecular Detection of Pathogens Leptospira in Common Rodent Captured in North Algeria Urban Areas |
title_full_unstemmed |
First Molecular Detection of Pathogens Leptospira in Common Rodent Captured in North Algeria Urban Areas |
title_sort |
first molecular detection of pathogens leptospira in common rodent captured in north algeria urban areas |
publisher |
HAL CCSD |
publishDate |
2022 |
url |
https://hal.inrae.fr/hal-03882346 https://doi.org/10.3390/tropicalmed7110335 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
ISSN: 2414-6366 Tropical Medicine and Infectious Disease https://hal.inrae.fr/hal-03882346 Tropical Medicine and Infectious Disease, 2022, 7 (11), pp.335. ⟨10.3390/tropicalmed7110335⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3390/tropicalmed7110335 info:eu-repo/semantics/altIdentifier/pmid/36355878 hal-03882346 https://hal.inrae.fr/hal-03882346 doi:10.3390/tropicalmed7110335 PUBMED: 36355878 WOS: 000881601300001 |
op_rights |
http://creativecommons.org/licenses/by/ |
op_doi |
https://doi.org/10.3390/tropicalmed7110335 |
container_title |
Tropical Medicine and Infectious Disease |
container_volume |
7 |
container_issue |
11 |
container_start_page |
335 |
_version_ |
1790607234282029056 |