Detection of the protistan parasite, Haplosporidium costale in Crassostrea gigas oysters from the French coast: A retrospective study
International audience The parasite Haplosporidium costale is known to infect and cause mortality in the oyster Crassostrea virginica in the USA. Decades after its first description in the 1960s, this parasite was detected in Crassostrea gigas in the USA and China. However, it presented a low preval...
Published in: | Journal of Invertebrate Pathology |
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ftccsdartic:oai:HAL:hal-03834632v1 2023-12-17T10:29:05+01:00 Detection of the protistan parasite, Haplosporidium costale in Crassostrea gigas oysters from the French coast: A retrospective study Cherif-Feildel, Maëva Lagy, Coralie Quesnelle, Yann Bouras, Hélène Trancart, Suzanne Houssin, Maryline LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) 2022-11 https://normandie-univ.hal.science/hal-03834632 https://doi.org/10.1016/j.jip.2022.107831 en eng HAL CCSD info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jip.2022.107831 hal-03834632 https://normandie-univ.hal.science/hal-03834632 doi:10.1016/j.jip.2022.107831 ISSN: 0022-2011 EISSN: 0022-2011 Journal of Invertebrate Pathology https://normandie-univ.hal.science/hal-03834632 Journal of Invertebrate Pathology, 2022, 195, pp.107831. ⟨10.1016/j.jip.2022.107831⟩ Real-time PCR Actin gene Rapid detection Haplosporidium costale Crassostrea gigas [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2022 ftccsdartic https://doi.org/10.1016/j.jip.2022.107831 2023-11-19T00:01:12Z International audience The parasite Haplosporidium costale is known to infect and cause mortality in the oyster Crassostrea virginica in the USA. Decades after its first description in the 1960s, this parasite was detected in Crassostrea gigas in the USA and China. However, it presented a low prevalence and no mortality was associated with it. More recently, in 2019, H. costale was detected in France in a batch of moribund oysters. In order to observe how long this parasite has been present on French coasts, from Normandy to Thau lagoon, a retrospective investigation was conducted on 871 adult and spat oyster batches from 2004 to 2020. To allow rapid detection on a large panel of samples, a real-time PCR for the H. costale actin gene was developed. This method allowed the detection of H. costale DNA in adults from 2005 and in spat from 2008. The H. costale prevalence in spat appeared higher than in adults over the years studied, 14.59 % compared to 6.50 %, respectively. All samples presenting positive results were then sequenced on two targets, H. costale rRNA and actin genes. The actin gene sequencing highlighted the presence of two H. costale strains. Adult C. gigas as well as spat batches coming from hatcheries and DNA controls from C. virginica all presented with the Profile 1 H. costale strain. The Profile 2 H. costale strain was detected only in C. gigas spat coming from natural sources. These observations suggest a correlation between the origin of oysters and H. costale strains which may have been caused by commercial imports between Japan, USA and France back to the 1970s. Over the positive samples studied, only few batches (n = 3) suffered mortalities which could be hypothesized to be caused by H. costale, all presenting the Profile 1 H. costale strain. Article in Journal/Newspaper Crassostrea gigas Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Journal of Invertebrate Pathology 195 107831 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Real-time PCR Actin gene Rapid detection Haplosporidium costale Crassostrea gigas [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology [SDV]Life Sciences [q-bio] |
spellingShingle |
Real-time PCR Actin gene Rapid detection Haplosporidium costale Crassostrea gigas [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology [SDV]Life Sciences [q-bio] Cherif-Feildel, Maëva Lagy, Coralie Quesnelle, Yann Bouras, Hélène Trancart, Suzanne Houssin, Maryline Detection of the protistan parasite, Haplosporidium costale in Crassostrea gigas oysters from the French coast: A retrospective study |
topic_facet |
Real-time PCR Actin gene Rapid detection Haplosporidium costale Crassostrea gigas [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology [SDV]Life Sciences [q-bio] |
description |
International audience The parasite Haplosporidium costale is known to infect and cause mortality in the oyster Crassostrea virginica in the USA. Decades after its first description in the 1960s, this parasite was detected in Crassostrea gigas in the USA and China. However, it presented a low prevalence and no mortality was associated with it. More recently, in 2019, H. costale was detected in France in a batch of moribund oysters. In order to observe how long this parasite has been present on French coasts, from Normandy to Thau lagoon, a retrospective investigation was conducted on 871 adult and spat oyster batches from 2004 to 2020. To allow rapid detection on a large panel of samples, a real-time PCR for the H. costale actin gene was developed. This method allowed the detection of H. costale DNA in adults from 2005 and in spat from 2008. The H. costale prevalence in spat appeared higher than in adults over the years studied, 14.59 % compared to 6.50 %, respectively. All samples presenting positive results were then sequenced on two targets, H. costale rRNA and actin genes. The actin gene sequencing highlighted the presence of two H. costale strains. Adult C. gigas as well as spat batches coming from hatcheries and DNA controls from C. virginica all presented with the Profile 1 H. costale strain. The Profile 2 H. costale strain was detected only in C. gigas spat coming from natural sources. These observations suggest a correlation between the origin of oysters and H. costale strains which may have been caused by commercial imports between Japan, USA and France back to the 1970s. Over the positive samples studied, only few batches (n = 3) suffered mortalities which could be hypothesized to be caused by H. costale, all presenting the Profile 1 H. costale strain. |
author2 |
LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) |
format |
Article in Journal/Newspaper |
author |
Cherif-Feildel, Maëva Lagy, Coralie Quesnelle, Yann Bouras, Hélène Trancart, Suzanne Houssin, Maryline |
author_facet |
Cherif-Feildel, Maëva Lagy, Coralie Quesnelle, Yann Bouras, Hélène Trancart, Suzanne Houssin, Maryline |
author_sort |
Cherif-Feildel, Maëva |
title |
Detection of the protistan parasite, Haplosporidium costale in Crassostrea gigas oysters from the French coast: A retrospective study |
title_short |
Detection of the protistan parasite, Haplosporidium costale in Crassostrea gigas oysters from the French coast: A retrospective study |
title_full |
Detection of the protistan parasite, Haplosporidium costale in Crassostrea gigas oysters from the French coast: A retrospective study |
title_fullStr |
Detection of the protistan parasite, Haplosporidium costale in Crassostrea gigas oysters from the French coast: A retrospective study |
title_full_unstemmed |
Detection of the protistan parasite, Haplosporidium costale in Crassostrea gigas oysters from the French coast: A retrospective study |
title_sort |
detection of the protistan parasite, haplosporidium costale in crassostrea gigas oysters from the french coast: a retrospective study |
publisher |
HAL CCSD |
publishDate |
2022 |
url |
https://normandie-univ.hal.science/hal-03834632 https://doi.org/10.1016/j.jip.2022.107831 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0022-2011 EISSN: 0022-2011 Journal of Invertebrate Pathology https://normandie-univ.hal.science/hal-03834632 Journal of Invertebrate Pathology, 2022, 195, pp.107831. ⟨10.1016/j.jip.2022.107831⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jip.2022.107831 hal-03834632 https://normandie-univ.hal.science/hal-03834632 doi:10.1016/j.jip.2022.107831 |
op_doi |
https://doi.org/10.1016/j.jip.2022.107831 |
container_title |
Journal of Invertebrate Pathology |
container_volume |
195 |
container_start_page |
107831 |
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1785581355417993216 |