Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg -BPI

• Published in: Proceedings of the National Academy of Sciences
• Main Authors: Gonzalez, Marcelo, Gueguen, Yannick, Destoumieux-Garzón, Delphine, Romestand, Bernard, Fievet, Julie, Pugnière, Martine, Roquet, Françoise, Escoubas, Jean-Michel, Vandenbulcke, Franck, Levy, Ofer, Sauné, Laure, Bulet, Philippe, Bachère, Evelyne
• Other Authors: Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire Grenoble (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2007
• Subjects: [SDV]Life Sciences [q-bio]; Crassostrea gigas
• Online Access: https://hal.archives-ouvertes.fr/hal-03828696; https://doi.org/10.1073/pnas.0702281104

International audience A cDNA sequence with homologies to members of the LPS-binding protein and bactericidal/permeability-increasing protein (BPI) family was identified in the oyster Crassostrea gigas . The recombinant protein was found to bind LPS, to display bactericidal activity against Escherichia coli , and to increase the permeability of the bacterial cytoplasmic membrane. This indicated that it is a BPI rather than an LPS-binding protein. By in situ hybridization, the expression of the C. gigas BPI ( Cg-bpi ) was found to be induced in hemocytes after oyster bacterial challenge and to be constitutive in various epithelia of unchallenged oysters. Thus, Cg-bpi transcripts were detected in the epithelial cells of tissues/organs in contact with the external environment (mantle, gills, digestive tract, digestive gland diverticula, and gonad follicles). Therefore, Cg -BPI, whose expression profile and biological properties are reminiscent of mammalian BPIs, may provide a first line of defense against potential bacterial invasion. To our knowledge, this is the first characterization of a BPI in an invertebrate.