ADAR-Editing during Ostreid Herpesvirus 1 Infection in Crassostrea gigas : Facts and Limitations

International audience Ostreid herpesvirus-1 (OsHV-1) RNAs are enzymatically modified by A-to-I conversions during the infection of Crassostrea gigas. The increase of ADAR1 expression and hyper-editing activity parallel to OsHV-1 RNAs suggests a functional connection between dsRNA editing and antivi...

Full description

Bibliographic Details
Published in:mSphere
Main Authors: Rosani, Umberto, Bortoletto, Enrico, Montagnani, C., Venier, Paola
Other Authors: Università degli Studi di Padova = University of Padua (Unipd), Interactions Hôtes-Pathogènes-Environnements (IHPE), Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2022
Subjects:
ADAR1
hyper-editing
oyster
OsHV-1
antiviral immunity
ADAR
RNA editing
bivalve
innate immunity
malacoherpesvirus
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Crassostrea gigas
Online Access:https://hal.science/hal-03636839
https://hal.science/hal-03636839/document
https://hal.science/hal-03636839/file/Rosani-2022-MSphere-ADAR.pdf
https://doi.org/10.1128/msphere.00011-22
Description
Summary:International audience Ostreid herpesvirus-1 (OsHV-1) RNAs are enzymatically modified by A-to-I conversions during the infection of Crassostrea gigas. The increase of ADAR1 expression and hyper-editing activity parallel to OsHV-1 RNAs suggests a functional connection between dsRNA editing and antiviral responses. We analyzed 87 RNA-seq data sets from immuno-primed, resistant, and susceptible oysters exposed to OsHV-1 to compare the ADAR hyper-editing levels on host and viral transcripts and trace hyper-editing on the oyster genes. Host RNAs were more hyper-edited than viral RNAs, despite the increased editing of viral RNAs in late infection phases. A set of genes, representing ∼0.5% of the oyster transcriptome and including several tripartite motif-containing sequences, were constantly hyper-edited. Conversely, we identified genes involved in antiviral response, miRNA maturation, and epigenetic regulation that were hyper-edited in specific conditions only. Despite technical and biological bottlenecks that hamper the understanding of the bivalve “RNA editome,” available tools and technologies can be adapted to bivalve mollusks

Similar Items